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Muhson, Nanang
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Physics

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Prevalence Analysis of Hypodermal Infectious and Haematopoetic Necrosis Virus (IHHNV) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Fahmi, Adzra Ariesta; Feliatra, Feli; Eff, Irwan; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The disease is a limiting factor and causes economic losses in the cultivation of white shrimp (Litopenaeus vannamei). Diseases in vaname shrimp are caused by viruses, microbes, parasites, organisms, and natural conditions. One of these diseases is infectious hypodermal and hematopoietic necrosis (IHHNV). This study was conducted from February to April 2022. This study aimed to analyze the prevalence of IHHNV that attacks white shrimp in the Bengkalis Regency and to observe and observe clinical symptoms in whitewashed shrimp infected with IHHNV in the pond area of the Bengkalis Regency. The research method used in this study is a survey method. The primary data taken included water quality observations, morphological observations, sampling, and detection of IHHNV using the polymerase chain reaction (PCR) method. The results showed that the prevalence of IHHNV in white shrimp was 0%. PCR testing using a specific primer measuring 392bp showed that white shrimp samples from three locations in Bengkalis District, namely Bengkalis District, Bukit Batu District, and Rupat District, were negative for IHHNV and did not show any clinical symptoms of IHHNV on morphological examination
Prevalence Analysis of WSSV (White Spot Syndrome Virus) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Akbar, Sidiq Malik; Effendi, Irwan; Feliatra, Feli; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp (Litopenaeus vannamei) is one type of shrimp that is widely cultivated in Indonesia. Vannamei shrimp dominates aquaculture in Indonesia which was previously dominated by tiger shrimp (Penaeus monodon). WSSV, a double-stranded circular DNA virus, has become one of the most widespread and fatal diseases in shrimp populations. Therefore, it is necessary to conduct research to detect the WSSV virus that attacks white vaname shrimp and determine the prevalence rate of the WSSV virus that attacks white vaname shrimp in Bengkalis Regency. This study was conducted from February to June 2022. The purpose of this study was to analyze the prevalence rate of the WSSV virus that attacks white shrimp in the Bengkalis Regency. The research method used in this study is a survey method. The results of the morphological examination did not show any clinical symptoms in vaname shrimp. Tests using PCR with specific primers measuring 941 bp showed DNA bands of vaname shrimp samples from 3 locations, namely Bengkalis district, Bukit Batu district and Rupat district. So the prevalence of the research results obtained is 0%.
Prevalence Analysis of Acute Hepatopancreatic Necrosis Disease (AHPND) in Vannamei Shrimp (Litopenaeus vannamei) by PCR Method in Bengkalis District Ayunda, Fadhilah Putri; Feliatra, Feli; Thamrin, Thamrin; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 4 No. 3 (2023): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp is one of the shrimps that are being cultivated in Indonesia because it has superior quality compared to other types of shrimp. Cultivation activities cannot be separated from disease attacks that attack cultured commodities. One of the diseases that attack vannamei shrimp is Acute Hepatopancreatic Necrosis Disease (AHPND). The disease is caused by Vibrio parahaemolyticus bacterial infection (VpAHPND) which produces toxins and causes death in shrimp with mortality reaching 100%. Therefore, it is necessary to conduct research or early detection in the aquaculture area of Bengkalis Regency using the PCR (Polymerase Chain Reaction) method. The method used is the survey method. This research was conducted from March to April 2023. The results of this study are the prevalence of AHPND (Acute Hepatopancreatic Necrosis Disease) in vannamei shrimp (Litopenaeus vannamei) in the Bengkalis Regency Aquaculture area is 0% with the category of infection never. AHPND examination by PCR method using AP4 primer measuring 230 bp showed that vannamei shrimp samples from Bengkalis District, Bantan District, and Bukit Batu District of Bengkalis Regency were negative for AHPND. The morphological condition of vannamei shrimp from the three research locations did not show any clinical symptoms of shrimp infected with AHPND
Prevalence Analysis of Acute Hepatopancreatic Necrosis Disease (AHPND) in Vannamei Shrimp (Litopenaeus vannamei) by PCR Method in Bengkalis District Ayunda, Fadhilah Putri; Feliatra, Feli; Thamrin, Thamrin; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 4 No. 3 (2023): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp is one of the shrimps that are being cultivated in Indonesia because it has superior quality compared to other types of shrimp. Cultivation activities cannot be separated from disease attacks that attack cultured commodities. One of the diseases that attack vannamei shrimp is Acute Hepatopancreatic Necrosis Disease (AHPND). The disease is caused by Vibrio parahaemolyticus bacterial infection (VpAHPND) which produces toxins and causes death in shrimp with mortality reaching 100%. Therefore, it is necessary to conduct research or early detection in the aquaculture area of Bengkalis Regency using the PCR (Polymerase Chain Reaction) method. The method used is the survey method. This research was conducted from March to April 2023. The results of this study are the prevalence of AHPND (Acute Hepatopancreatic Necrosis Disease) in vannamei shrimp (Litopenaeus vannamei) in the Bengkalis Regency Aquaculture area is 0% with the category of infection never. AHPND examination by PCR method using AP4 primer measuring 230 bp showed that vannamei shrimp samples from Bengkalis District, Bantan District, and Bukit Batu District of Bengkalis Regency were negative for AHPND. The morphological condition of vannamei shrimp from the three research locations did not show any clinical symptoms of shrimp infected with AHPND.
Prevalence Analysis of Hypodermal Infectious and Haematopoetic Necrosis Virus (IHHNV) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Fahmi, Adzra Ariesta; Feliatra, Feli; Effendi, Irwan; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The disease is a limiting factor and causes economic losses in the cultivation of white shrimp (Litopenaeus vannamei). Diseases in vaname shrimp are caused by viruses, microbes, parasites, organisms, and natural conditions. One of these diseases is infectious hypodermal and hematopoietic necrosis (IHHNV). This study was conducted from February to April 2022. This study aimed to analyze the prevalence of IHHNV that attacks white shrimp in the Bengkalis Regency and to observe and observe clinical symptoms in whitewashed shrimp infected with IHHNV in the pond area of the Bengkalis Regency. The research method used in this study is a survey method. The primary data taken included water quality observations, morphological observations, sampling, and detection of IHHNV using the polymerase chain reaction (PCR) method. The results showed that the prevalence of IHHNV in white shrimp was 0%. PCR testing using a specific primer measuring 392bp showed that white shrimp samples from three locations in Bengkalis District, namely Bengkalis District, Bukit Batu District, and Rupat District, were negative for IHHNV and did not show any clinical symptoms of IHHNV on morphological examination
Prevalence Analysis of WSSV (White Spot Syndrome Virus) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Akbar, Sidiq Malik; Effendi, Irwan; Feliatra, Feli; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp (Litopenaeus vannamei) is one type of shrimp that is widely cultivated in Indonesia. Vannamei shrimp dominates aquaculture in Indonesia which was previously dominated by tiger shrimp (Penaeus monodon). WSSV, a double-stranded circular DNA virus, has become one of the most widespread and fatal diseases in shrimp populations. Therefore, it is necessary to conduct research to detect the WSSV virus that attacks white vaname shrimp and determine the prevalence rate of the WSSV virus that attacks white vaname shrimp in Bengkalis Regency. This study was conducted from February to June 2022. The purpose of this study was to analyze the prevalence rate of the WSSV virus that attacks white shrimp in the Bengkalis Regency. The research method used in this study is a survey method. The results of the morphological examination did not show any clinical symptoms in vaname shrimp. Tests using PCR with specific primers measuring 941 bp showed DNA bands of vaname shrimp samples from 3 locations, namely Bengkalis district, Bukit Batu district and Rupat district. So the prevalence of the research results obtained is 0%.
Co-Authors Akbar, Sidiq Malik Ayunda, Fadhilah Putri Fahmi, Adzra Ariesta Feliatra, Feli Irwan Effendi Irwan Irwan Thamrin Thamrin