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All Journal Jurnal Perikanan dan Kelautan Jurnal Ilmu Perairan (Aquatic Science) Asian Journal of Aquatic Sciences Journal of Coastal and Ocean Sciences Journal of Rural and Urban Community Empowerment Abdimas Indonesian Journal Jurnal Perikanan dan Kelautan
Feliatra, Feli
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Social Sciences

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Growth of Bacteria Bacillus cereus in Liquid Media with Different Protein Sources Mukti, Pranata Rangga; Feliatra, Feli; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 1 No. 1 (2020): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The aim of this research was to determine the optimal media composition and incubation time for the growth of Bacillus cereus and B. cereus isolate consortium with other strains isolate. Using an experimental method, which was testing 2 groups of bacterial isolates; B. cereus strain SN7 (isolate N) and bacterial consortium (combination of 5 isolates of B. cereus with different strains). Each group of isolates was grown in sago waste media added with two different protein sources (egg whites and skim milk) and 3 different concentrations in each protein source (8%, 10%, and 12%) with 3 replications in each treatment. Bacterial culture was measured every 6 hours for 24 hours using three methods; total agar plate count, spectrophotometric methods, and bacterial cell biomass methods. There was no significant difference between the growth of these two groups of bacterial isolates. Optimal growth of each isolate was in the sago waste media which was added with egg white but with different concentrations, namely 10% in B. cereus SN7 and 12% in the consortium. B. cereus consortium showed higher growth than B. cereus SN7. The optimal time for bacteria to grow was in the range of 12 to 24 hours
Growth of Bacteria Bacillus cereus in Liquid Mediums with Different Carbohydrate Sources Ningsih, Irma Ardila; Feliatra, Feli; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 1 No. 1 (2020): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Bacteria need a medium to grow Bacillus cereus is a probiotic bacterium. Molasses and tofu liquid waste are one of the wastes that are used as alternative medium, because they are easy to obtain, cheap, and contain nutrients that are high enough in the form of carbohydrates. This study aimed to determine the optimal medium for the growth of these bacteria. This study used a completely randomized factorial design. It consists of three factors, namely the type of waste (molasses + egg albumen and tofu liquid waste + egg albumen), then the combination of isolates (B. cereus SN7 and consortium), and the concentration of liquid waste used for medium growth is (molasses is 1%; 1.5% ; and 2% while tofu liquid waste is 8%; 10%; and 12%). Bacterial cell growth was measured by the Total Plate Count (TPC) method, spectrophotometric method, and biomass, while the incubation period for cell growth was measured every 0, 6, 12, 18, and 24 hours. The results of this study indicated that molasses + egg albumen with a concentration of 1% was the best medium for the growth of B. cereus bacteria along with consortium isolates
Prevalence Analysis of Hypodermal Infectious and Haematopoetic Necrosis Virus (IHHNV) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Fahmi, Adzra Ariesta; Feliatra, Feli; Eff, Irwan; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The disease is a limiting factor and causes economic losses in the cultivation of white shrimp (Litopenaeus vannamei). Diseases in vaname shrimp are caused by viruses, microbes, parasites, organisms, and natural conditions. One of these diseases is infectious hypodermal and hematopoietic necrosis (IHHNV). This study was conducted from February to April 2022. This study aimed to analyze the prevalence of IHHNV that attacks white shrimp in the Bengkalis Regency and to observe and observe clinical symptoms in whitewashed shrimp infected with IHHNV in the pond area of the Bengkalis Regency. The research method used in this study is a survey method. The primary data taken included water quality observations, morphological observations, sampling, and detection of IHHNV using the polymerase chain reaction (PCR) method. The results showed that the prevalence of IHHNV in white shrimp was 0%. PCR testing using a specific primer measuring 392bp showed that white shrimp samples from three locations in Bengkalis District, namely Bengkalis District, Bukit Batu District, and Rupat District, were negative for IHHNV and did not show any clinical symptoms of IHHNV on morphological examination
Prevalence Analysis of WSSV (White Spot Syndrome Virus) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Akbar, Sidiq Malik; Effendi, Irwan; Feliatra, Feli; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp (Litopenaeus vannamei) is one type of shrimp that is widely cultivated in Indonesia. Vannamei shrimp dominates aquaculture in Indonesia which was previously dominated by tiger shrimp (Penaeus monodon). WSSV, a double-stranded circular DNA virus, has become one of the most widespread and fatal diseases in shrimp populations. Therefore, it is necessary to conduct research to detect the WSSV virus that attacks white vaname shrimp and determine the prevalence rate of the WSSV virus that attacks white vaname shrimp in Bengkalis Regency. This study was conducted from February to June 2022. The purpose of this study was to analyze the prevalence rate of the WSSV virus that attacks white shrimp in the Bengkalis Regency. The research method used in this study is a survey method. The results of the morphological examination did not show any clinical symptoms in vaname shrimp. Tests using PCR with specific primers measuring 941 bp showed DNA bands of vaname shrimp samples from 3 locations, namely Bengkalis district, Bukit Batu district and Rupat district. So the prevalence of the research results obtained is 0%.
Prevalence Analysis of Acute Hepatopancreatic Necrosis Disease (AHPND) in Vannamei Shrimp (Litopenaeus vannamei) by PCR Method in Bengkalis District Ayunda, Fadhilah Putri; Feliatra, Feli; Thamrin, Thamrin; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 4 No. 3 (2023): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp is one of the shrimps that are being cultivated in Indonesia because it has superior quality compared to other types of shrimp. Cultivation activities cannot be separated from disease attacks that attack cultured commodities. One of the diseases that attack vannamei shrimp is Acute Hepatopancreatic Necrosis Disease (AHPND). The disease is caused by Vibrio parahaemolyticus bacterial infection (VpAHPND) which produces toxins and causes death in shrimp with mortality reaching 100%. Therefore, it is necessary to conduct research or early detection in the aquaculture area of Bengkalis Regency using the PCR (Polymerase Chain Reaction) method. The method used is the survey method. This research was conducted from March to April 2023. The results of this study are the prevalence of AHPND (Acute Hepatopancreatic Necrosis Disease) in vannamei shrimp (Litopenaeus vannamei) in the Bengkalis Regency Aquaculture area is 0% with the category of infection never. AHPND examination by PCR method using AP4 primer measuring 230 bp showed that vannamei shrimp samples from Bengkalis District, Bantan District, and Bukit Batu District of Bengkalis Regency were negative for AHPND. The morphological condition of vannamei shrimp from the three research locations did not show any clinical symptoms of shrimp infected with AHPND
GROWTH OF HETEROTROPHIC BACTERIA IN SEA WATER POLLUTED BY SURF DETERGENT Novan, Dewan Pratama; Effendi, Irwan; Feliatra, Feli
Asian Journal of Aquatic Sciences Vol. 1 No. 1 (2018): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.124 KB)

Abstract

The research was conducted from February 2016 to April 2017, aimed to determine the effect of adding different concentrations of detergent namely SURF on the growth of bacterial populations in the seawater column. This research used experimental method with Completely Randomized Design. Seawater samples were analyzed at Marine Microbiology Laboratory, Fisheries and Marine Faculty of Riau University. Based on the results of the study, from day 0 untill day 10 after given detergent with different concentrations, bacteria experienced a decreasing trend of growth, in day 15 untill day 20, the trend of bacterial growth contaminated by detergent with different concentration relatively increased to stagnant. Based on the result of this study the addition of different detergent concentration in each treatment affect the growth of heterotrophic bacteria population in the sea water, where the higher concentration of detergent added the lower the ability of bacteria to grow and the ability of heterotrophic bacteria to survive at different concentrations of detergent, it can be concluded that the ability of heterotrophic bacteria to grow from days 0 to 20 has decreased in each of its concentrations
EFFECTIVENESS TEST OF NIPAH EXTRACT AS LARVACIDE ON MOSQUITO LARVAE (Aedes aegpti) Purba, Thadeus Mariode Triputra; Effendi, Irwan; Feliatra, Feli
Asian Journal of Aquatic Sciences Vol. 2 No. 1 (2019): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.705 KB)

Abstract

This research was conducted in January - February 2019. The purpose of this study was to examine the effectiveness of mangrove extracts on stems and fruit on the growth of Aedes aegypti mosquito larvae and to know Lethal Concentration LC50 and Lethal Time LT50. The method used is the experimental method, with the experimental design is a completely randomized design (CRD). The treatment of nipah fruit and stem extract concentrations was 3%, 6% and 9% with contact time of 15 minutes, 30 minutes, 45 minutes 60 minutes, 120 minutes, 480 minutes and the number of replications 3 times with 2 trials. To find out (LC50) and (LT50), the data was analyzed using the SPSS program. The sample of this research is stem and nipah fruit taken in the mangrove area of Kayu Ara Village, Siak Regency. Aedes aegypti larvae are taken at a water reservoir next to the Arfaunas Mosque in Tampan District. The results showed that the stem and nipah fruit extracts had effective killing power against Aedes aegpti larvae. The extract that was more effective in killing mosquito larvae was the nipah fruit extract compared to the nipah stem extract seen in the number of larval deaths with LC50 = 3.398% and LT50 = 3.540 hours. This shows that the level of toxicity is quite toxic.
EFFECTIVITY OF NIPAH (Nypa fruticans) EXTRACT ON (Aedes aegypti) LARVAE IN BRACKISH WATER Rady, Rady; Effendi, Irwan; Feliatra, Feli
Asian Journal of Aquatic Sciences Vol. 2 No. 2 (2019): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (639.546 KB)

Abstract

The most commonly used method of vektor control is temephos (the synthetic larvicide). Currently in some areas there has been resistance of aedes aegypti larvae to temephos, so that natural larvicide is required as an alternative. Nypa exctract contains flavonoid compounds that are respiratory toxins that can kill aedes aegypty larvae.This research was conducted in January - February 2019. The purpose of this study was to determine the inhibitory power of nipah extract against larvae in brackhish water. The results of this study are expected to increase knowledge and provide information about the pontential of nipah as a soursce of bioactive compounds. The method used is the experimental method, where the location of the research sampel was obtained at one sampling location point in the Sungai Kayu Ara village, Siak Riau province. The results showed that the average mortality of larvae in nipah laeves in the 60th minute was 55, 80, 100 and 100%. While root extracts have larvae deaths as many as 60 minutes, namely 100, 90, 90, and 100%. For the average mortality of larvae at the root of nipah there are 60 minutes, which are 95, 100, 100 and 100%. As wel as positive and negative controls as a comparison. So it can be compared to 3 administration of nipah extract both leaves, roots and midrib so that the three conncentrations used are better than the use of midrib extract compared to leaf and root extract. It can be concluded that the more concentration used the higher the mortality of larvae is the concentration of 12%. For the concentration used the percentage of larvae mortality in the test media is as much as 6%, 9% and 12%. Every death of larvae has reristance to different bodies so that at the 30th minute there is a difference in mortality of larvae wich a given concentration. The larvae species used are aedes aegyti larvae. The highest mortality of dead es was found in midrib extract and also positive controls. So that the death of larvae is very effective against the given solution has the same ability. The mortality of A. aegypti larvae were then analyzed using probit analysis to obtain LC50 (Lethal Concentration 50) and LT50 (Lethal Time 50) values. The results of the probit analysis of leaf, root and midrib extracts are the estimation of LC50 1.493, 2.618, 0.005. while leaf , root and midrib extracts at the LT50 estimate were 2.841, 1.579, 2.227
ADDITION OF SUPERIOR HETEROTROF BACTERIA MIXED IN FEED TO IMPROVE HEALTH OF SALINE TILAPIA Oreochromis niloticus Simanjuntak, Lewi Maraditha; Lukistyowati, Iesje; Feliatra, Feli
Asian Journal of Aquatic Sciences Vol. 2 No. 3 (2019): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (915.08 KB)

Abstract

This research was conducted in May 2019 until July 2019 which took place at the Laboratory of Marine Microbiology and the Laboratory of Fish Parasites and Diseases at the Faculty of Fisheries and Maritime Affairs, Riau University. The purpose of this study was to determine the effect of the addition of superior heterotrophic bacterial isolates that were sprayed on feed could affect the health of saline tilapia (Oreochromis niloticus) through erythrocyte and leukocyte blood images. The treatments are Kn, Kp, feed sprayed with B.cereus heterotrophic bacterial isolate solution (0.15% dose), feed sprayed with heterotrophic V. fluvialis bacterial isolate solution (0.15% dose), feed sprayed with combined Isolate solution. The results of the study of the addition of superior heterotrophic bacterial isolates sprayed on feed can improve the health of saline tilapia (Oreochromis niloticus) after 30 days of maintenance and post-infection of Aeromonas hydrophila bacteria showed that the treatment of P3 (combined isolates) which is the best treatment with a total leukocyte value of 89.67 x 103 cells / mm3, lymphocytes 80.33%, neutrophils 10.67%, monocytes 9.66%, total erythrocytes 235.00 x 104 cells / mm3, hematocrit 32.00% and hemoglobin 7.33 g / dL and weight growth absolute 7.05 g, a survival rate of 83.33%.
ISOLATION AND ANTAGONISM OF BACTERIA TEST OF BIOTA IN THE MANGROVE ECOSYSTEM KAYU ARA RIVER SIAK REGENCY Moritania, Rizky; Effendi, Irwan; Feliatra, Feli
Asian Journal of Aquatic Sciences Vol. 2 No. 3 (2019): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (930.339 KB)

Abstract

The General area is the mangrove biodiversity and marine life that have biological activity that likely has the same pharmacological potential as an antimicrobial compound. The purpose of this research is to know the bacterial antagonism in isolation from mangrove, mangrove crabs, snails and fish tembakul against pathogenic bacteria (Aeromonas hydrophila, Aeromonas salmonicida, Edwardsiella ictaluri). This research dimula from May – June 2019. From the results of research that has been done shows that isolates that have the capability of antagonism against pathogenic bacteria test three (A. salmonicida, A. hydrophila and E. ictaluri) were isolate CS7 with category stronger, isolate CS6, CS5, CS4, AI4, AI5, CS9 AI2, with weak category, whereas isolates CS1, B22, B33, B44, AI6 AI7 AI8, was only able to inhibit pathogenic test of 2 with a weak category. The results of the DNA analysis of the 16S rRNA sequence with the use and analysis of BLAST obtained that isolates the Bacillus cereus species are included.
Co-Authors Akbar, Sidiq Malik Alfarisy, M. Phyto Ayunda, Fadhilah Putri Batara, Lamun Batubara, Ummi Mardhiah Bintal Amin Dessy Yoswaty Fahmi, Adzra Ariesta Farisy, Muhammad Fhito Al Fatmanita, Fatmanita Fatwa, Eza Buana Feliatra, Pamela Isya Gustina, Julia Iesje Lukistyowati Irvina Nurachmi Irwan Effendi Irwan Irwan Lazuardi Umar Mardalisa, Mardalisa Moritania, Rizky Muhson, Nanang Mukti, Pranata Rangga Ningsih, Irma Ardila Novan, Dewan Pratama Nursyirwani, Nursyirwani Prastyo, Nanang Tri Purba, Thadeus Mariode Triputra Rady, Rady Simanjuntak, Lewi Maraditha Syafruddin Nasution Thamrin Thamrin Zevira, Bella Permata