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Irmawati Purbo Astuti, Ike
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STUDI LEPTOSPIRA SP PADA BEBERAPA DAERAH RAWAN BANJIR DI JAKARTA Widiyanti, S.Si, M.Si, PhD, Dian; Irmawati Purbo Astuti, Ike
Jurnal Kedokteran YARSI Vol 24, No 2 (2016): MEI - AGUSTUS 2016
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v24i2.121

Abstract

Leptospirosis termasuk re-emerging disease dan sering menjadi wabah setelah bencana banjir. Penyakit ini disebabkan oleh bakteri Leptospira patogen yang ditansmisikan secara langsung lewat hewan terinfeksi atau tidak langsung melalui lingkungan yang terkontaminasi urin hewan tersebut. Studi mengenai Leptospira yang ada di lingkungan perairan daerah rawan banjir dilakukan untuk mengetahui penyebaran Leptospira, terutama strain patogen, sehingga dapat dilakukan antisipasi pencegahan. Sampel dikumpulkan dan diukur pHnya dari 20 penampungan air, seperti waduk, danau, sungai, selokan air, di daerah rawan banjir di Jakarta, dan dikultur pada medium Korthof modifikasi mengandung 5-fluorouracil. Pengamatan hasil kultur dilakukan dengan mikroskop lapang gelap selama satu bulan. Diferensiasi Leptospira dilakukan dengan deteksi gen flaB. Hasil menunjukkan bahwa 75% dari sampel yang diperoleh, positif Leptospira. Ph sampel air sebesar 6,6?7,9 masih sesuai untuk pertumbuhan Leptospira. Analisis dengan gen flaB menunjukkan bahwa Leptospira yang diisolasi termasuk jenis saprofit. 
Comparison of Airborne Flora Before and After Learning Activities in Skill Lab and Tutorial Rooms at the Faculty of Medicine, Yarsi University, and a Review From an Islamic Perspective Lintang Ramadhani, Himaulya; Irmawati Purbo Astuti, Ike; Djannatun, Titiek
Jurnal Indonesia Sosial Sains Vol. 7 No. 2 (2026): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v7i2.2224

Abstract

Indoor Air Quality (IAQ) significantly affects health and concentration in educational environments. Factors such as learning activities, population density, and ventilation systems influence the presence of airborne microorganisms. This research aims to compare the airborne flora before and after learning activities in the Skill Lab and Tutorial rooms at the Faculty of Medicine, YARSI University, and to analyze the findings from an Islamic perspective. A descriptive qualitative–quantitative method was applied, with 20 samples collected using the settle plate method on Nutrient Agar media. Samples were incubated at 35–37°C for 48 hours, and the bacterial colonies were then counted (CFU) and identified using Gram staining. The results show that the number of airborne microorganisms in five Skill Labs and five Tutorial rooms remained below the standard threshold for air quality (<700 CFU/m³). However, there was a noticeable increase in the number of colonies after learning activities, with the lowest value recorded at 0 CFU/m³ and the highest at 13 CFU/m³. This increase is suspected to be related to human activity, density, and mobility during class sessions. Microscopic identification revealed the predominance of Gram-positive Coccus bacteria, with some Gram-negative rods. In conclusion, the number of airborne microorganisms in the Skill Lab and Tutorial rooms at the Faculty of Medicine, YARSI University, is still within the quality standard, although learning activities contribute to an increase in colony numbers. Therefore, optimal management of the room environment is necessary to maintain good air quality.
Identification of Airborne Flora Before and After Activities in the Libraryand Classrooms of the Faculty of Medicine, Yarsi University, and a Review From an Islamic Perspective Hasna Kamalah, Maitsa; Irmawati Purbo Astuti, Ike; Djannatun, Titiek
Jurnal Indonesia Sosial Sains Vol. 7 No. 2 (2026): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v7i2.2225

Abstract

Libraries and classrooms are indoor environments with high usage intensity, making Indoor Air Quality (IAQ) crucial for the health and comfort of occupants. Indoor air flora is influenced by various environmental factors, including human activities, occupant density, temperature, humidity, lighting, and ventilation. Airborne microorganisms can act as vectors for pathogens that impact health; therefore, monitoring air flora before and after activities is essential. This study employed a descriptive quantitative–qualitative approach. A total of 20 air samples were collected from the library and classrooms using the settle plate method with Nutrient Agar media, both before and after activities. Samples were incubated at 37°C for 24–48 hours, after which bacterial colonies were counted (CFU) and identified based on colony morphology and Gram staining. The results showed an increase in airborne colonies after activities, particularly in classrooms, with the highest count observed in Class 10A. The library exhibited relatively fewer colonies, remaining below the standard IAQ threshold of 700 CFU/m³. Microbial identification revealed that Gram-negative Cocci dominated in classrooms, while Gram-positive Cocci were more prevalent in the library. These results indicate that human activities contribute significantly to the increase in airborne microorganisms. In conclusion, high occupancy rates and activity levels in classrooms lead to higher microbial loads, whereas libraries maintain lower counts. Human activity directly affects the concentration of indoor air microorganisms. To support a healthy and comfortable learning environment, effective hygiene management and optimal ventilation systems are essential for maintaining good Indoor Air Quality.
Co-Authors Dian Widiyanti Hasna Kamalah, Maitsa Lintang Ramadhani, Himaulya TITIEK DJANNATUN