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Pemanfaatan darah manusia yang kadaluarsa sebagai pengganti darah domba dalam pembuatan media Agar Darah Plat (ADP) Djannatun, Titiek; T Rochani, Jekti; Wikaningrum, Riyani; Widiyanti, Dian; Rahim Pane, Abdul
Jurnal Kedokteran YARSI Vol 16, No 2 (2008): MEI - AGUSTUS 2008
Publisher : Lembaga Penelitian Universitas YARSI

The use of blood agar media to grow and to isolate the pathogenic bacteria, and to compare the hemolytic ability of the bacteria is well known. The aim of this study was to investigate the possibility of using expired human blood as substitute for the sheep blood agar media. In this study five days before expired and five days after expired human blood were washed once, twice and centrifuged. Sheep blood was used as positive control and five days after and before expired blood without treatment was used as negative control. The prepared media were used to grow six isolates wild strain bacteria (Staphylococcus aureus, Streptococcus B haemolyticus,Streptococcus y haemolyticus, Streptococcus pneumoniae, Vibrio El Tor and Clostridium perfringens). The result indicated that blood agar medium, prepared using human blood which was nearly expired or expired, have the same results in culture growth of the tested bacteria compared to the standard blood agar had media using sheep blood.

POPULASI BAKTERI PADA FESES NEONATUS: PENELITIAN PENDAHULUAN Wikaningrum, Riyani; T Rochani, Jekti; Djannatun, Titiek; Widiyanti, Dian
Jurnal Kedokteran YARSI Vol 16, No 2 (2008): MEI - AGUSTUS 2008
Publisher : Lembaga Penelitian Universitas YARSI

Recent published data have outlined a relationship between the composition of the intestinal microflora and allergic inflammation, autoimmunity, chronic bowel inflammation, psychiatric, cognitive and behavioral disturbances. Factors influencing intestinal microflora are environment, genetic predisposition, diet, age, diseases, drugs, stressor. This preliminary study is to establish local reference of microbial flora in neonates. This study included samples taken from 20 neonates, age 1 ? 7 days, from a private hospital in Jakarta. All samples were sent to microbiology laboratory without transport media and processed immediately. Bacteriological cultures for aerob and anaerob bacteria were performed according to the standard methods. Aerob and anaerob bacterial species were isolated from all samples, ranged 2 ? 5 species per sample. Enterobacteriaceae were found to be dominant isolates (25% - 75%); followed by Streptococcus anhaemolyticus (60%), Staphylococcus epidermidis (40%), Staphylococcus aureus (5%),Clostridium difficile (5%), Bacteroides fragilis (5%), Bifidobacterium sp. (10%), Lactobacillus sp. (5%) and yeast (5%). These data indicated that aerob and facultative anaerob bacteria were predominant in neonates. However Bifidobacterium, Lactobacillus and Clostridium difficile could be found. Further studies are needed to confirm this finding using larger number of samples and involving various age group.

STUDI LEPTOSPIRA SP PADA BEBERAPA DAERAH RAWAN BANJIR DI JAKARTA Widiyanti, S.Si, M.Si, PhD, Dian; Irmawati Purbo Astuti, Ike
Jurnal Kedokteran YARSI Vol 24, No 2 (2016): MEI - AGUSTUS 2016
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v24i2.121

Leptospirosis termasuk re-emerging disease dan sering menjadi wabah setelah bencana banjir. Penyakit ini disebabkan oleh bakteri Leptospira patogen yang ditansmisikan secara langsung lewat hewan terinfeksi atau tidak langsung melalui lingkungan yang terkontaminasi urin hewan tersebut. Studi mengenai Leptospira yang ada di lingkungan perairan daerah rawan banjir dilakukan untuk mengetahui penyebaran Leptospira, terutama strain patogen, sehingga dapat dilakukan antisipasi pencegahan. Sampel dikumpulkan dan diukur pHnya dari 20 penampungan air, seperti waduk, danau, sungai, selokan air, di daerah rawan banjir di Jakarta, dan dikultur pada medium Korthof modifikasi mengandung 5-fluorouracil. Pengamatan hasil kultur dilakukan dengan mikroskop lapang gelap selama satu bulan. Diferensiasi Leptospira dilakukan dengan deteksi gen flaB. Hasil menunjukkan bahwa 75% dari sampel yang diperoleh, positif Leptospira. Ph sampel air sebesar 6,6?7,9 masih sesuai untuk pertumbuhan Leptospira. Analisis dengan gen flaB menunjukkan bahwa Leptospira yang diisolasi termasuk jenis saprofit.

Sensitivity of Staphylococcus aureus Isolated from Nasal and Throat of Pre-Clerkship Students to Antibiotics Rahayu, Suci; Widiyanti, Dian; Arsyad, Muhammad
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 20, No 2: July 2020
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mm.200251

Background: Cases of nosocomial infection are many caused by the bacteria ofÂ S.aureus. Bacteria infection caused by S.aureus may result in various skin and soft tissue infections. The infection may become difficult to treat if the strain ofÂ S.aureus responsible is the methicillin resistant strain or methicillin-resistant Staphylococcus aureus (MRSA). Currently there are two known types of MRSA: Hospital Acquired MRSA/HA-MRSA and Community Acquired MRSA/CA-MRSA. Transmission of the bacteria from one patient to another may be caused by unsterile medical equipment or from the hospital staff, especially pre-clinical students who will work in a hospital. The purpose of this study was to obtained the difference of bacteria colonisations S.aureus from nasal and throat swabs, and the sensitivity patterns of S.aureus when exposed to antibiotics (methicillin, vancomycin, imipenem, penicillin, ofloxacin, ciprofloxacin) and the prevalence of MRSA carriers and also to obtain an understanding of the characteristics of medical drug use through Islamic views. Metodes: This study used descriptive analysis method with samples taken from nasal and throat swabs applied to healthy pre-clinic student from Universitas YARSI. Statistical analysis using Mann-Whitney test in the program SPSS for windows. Results: The results bacteria colonisations S.aureus from nasal swabs was positive (23,3%), while from the throat sample was positive (10%). The results of statistical analysis (Sig. = 0.051 0.05) shows that there is no significant difference between the bacterial colonies of S.aureus originating from the nasal swabs and throat swabs of the pre-clinical students. From 60 samples a positive prevalence of (1.7%) was obtained for MRSA carriers. The result of the sensitivity test shows that the isolated sample of the bacteria S.aureus shows the highest sensitivity to the antibiotic imipenem and almost all the isolated samples show resistance to the antibiotic penicillin. Conclusion: There are more S.aureus bacteria colonies on nasal swabs compared to throat swabs. There was no significant difference between S.aureus bacteria colonization of nose and throat swabs in pre-clinical-students. The positive prevalence of MRSA carrier among pre-clinical-students of Yarsi University was (1.7%). In nasal swabs all isolates were still sensitive to the antibiotics methicillin, imipenem, ofloxacin and ciprofloxacin. But on the nasal swab there were three VISA isolates. In the throat swab, there was one MRSA isolate and one isolate that was resistant to ofloxacin. The inhibition zone of the highest sensitivity was obtained for imipenem antibiotics. Almost all isolates showed resistance to penicillin antibiotics. In Islamic medicine, drugs or antibiotics must not contain unclean and haram objects for human consumption.

Sensitivity of Staphylococcus aureus Isolated from Nasal and Throat of Pre-Clerkship Students to Antibiotics Rahayu, Suci; Widiyanti, Dian; Arsyad, Muhammad
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 20, No 2 (2020): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mm.200251

Background: Cases of nosocomial infection are many caused by the bacteria ofÂ S.aureus. Bacteria infection caused by S.aureus may result in various skin and soft tissue infections. The infection may become difficult to treat if the strain ofÂ S.aureus responsible is the methicillin resistant strain or methicillin-resistant Staphylococcus aureus (MRSA). Currently there are two known types of MRSA: Hospital Acquired MRSA/HA-MRSA and Community Acquired MRSA/CA-MRSA. Transmission of the bacteria from one patient to another may be caused by unsterile medical equipment or from the hospital staff, especially pre-clinical students who will work in a hospital. The purpose of this study was to obtained the difference of bacteria colonisations S.aureus from nasal and throat swabs, and the sensitivity patterns of S.aureus when exposed to antibiotics (methicillin, vancomycin, imipenem, penicillin, ofloxacin, ciprofloxacin) and the prevalence of MRSA carriers and also to obtain an understanding of the characteristics of medical drug use through Islamic views. Metodes: This study used descriptive analysis method with samples taken from nasal and throat swabs applied to healthy pre-clinic student from Universitas YARSI. Statistical analysis using Mann-Whitney test in the program SPSS for windows. Results: The results bacteria colonisations S.aureus from nasal swabs was positive (23,3%), while from the throat sample was positive (10%). The results of statistical analysis (Sig. = 0.051 0.05) shows that there is no significant difference between the bacterial colonies of S.aureus originating from the nasal swabs and throat swabs of the pre-clinical students. From 60 samples a positive prevalence of (1.7%) was obtained for MRSA carriers. The result of the sensitivity test shows that the isolated sample of the bacteria S.aureus shows the highest sensitivity to the antibiotic imipenem and almost all the isolated samples show resistance to the antibiotic penicillin. Conclusion: There are more S.aureus bacteria colonies on nasal swabs compared to throat swabs. There was no significant difference between S.aureus bacteria colonization of nose and throat swabs in pre-clinical-students. The positive prevalence of MRSA carrier among pre-clinical-students of Yarsi University was (1.7%). In nasal swabs all isolates were still sensitive to the antibiotics methicillin, imipenem, ofloxacin and ciprofloxacin. But on the nasal swab there were three VISA isolates. In the throat swab, there was one MRSA isolate and one isolate that was resistant to ofloxacin. The inhibition zone of the highest sensitivity was obtained for imipenem antibiotics. Almost all isolates showed resistance to penicillin antibiotics. In Islamic medicine, drugs or antibiotics must not contain unclean and haram objects for human consumption.

STUDY OF RISK FACTORS AND LEPTOSPIRA DETECTION OF SANITARY WORKERS IN JAKARTA, INDONESIA Muslimin Budiman; Syifa Melati Putri; Putri Medita Rachmayanti; Ramdesima Kasmir; Dian Widiyanti
Biomedika Vol 14, No 2 (2022): Biomedika Agustus 2022
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v14i2.17953

ABSTRACT Leptospirosis is zoonotic disease, caused by spirochete-bacteria Leptospira, transmitted by excreted urine of rodent into the environment. Sanitary workers had high-risk of Leptospira infection due to frequent exposure to contaminated environment, which could cause asymptomatic leptospiruria (the presence of leptospira in urine) and severe complications, such as chronic kidney disease. The aim of this study was to find leptospiruria in sanitary workers, using PCR method, targeting specific genes of Leptospira. Urine samples and questionnaires were obtained from fifteen sanitary workers. Samples were cultured in EMJH-broth with addition of 5-fluorouracil, incubated for 3 months and observed for growth of bacteria using dark-field microscope. Identification of bacteria was performed by PCR, targeting lipl32, rrl, flaB, ompL1 genes, followed by sequencing using Sanger method, alignment using ClustalW and BLAST. The questionnaires result showed that 26,7% of respondent had medium level of risk factors, and 53,3% of respondent had applied good prevention for leptospirosis. Pearson correlation analysis showed that there was relationship between risk factors and prevention. Culture result showed growth in four samples, and analysis by PCR only showed rrl-PCR had expected amplicon. However sequencing result showed that the amplicon had 99% homogeneity to Pseudomonas stutzeri. In conclusion, Leptospira was not found in the urine of sanitary worker, might be due to applied good prevention for leptospirosis.Keywords: Leptospira, Leptospiruria, Sanitary Worker, Risk Factors ABSTRAK Leptospirosis adalah penyakit zoonosis, yang disebabkan oleh bacteria Leptospira Sp., ditularkan melalui urin hewan pengerat yang dikeluarkan ke lingkungan. Pekerja sanitasi memiliki risiko tinggi terinfeksi Leptospira karena sering terpapar lingkungan yang terkontaminasi, yang dapat menyebabkan leptospiruria (adanya liptospira dalam urin) tanpa gejala, dan komplikasi berat, seperti penyakit ginjal kronis. Tujuan dari penelitian ini adalah untuk menemukan leptospiruria pada petugas kebersihan, menggunakan metode PCR, menargetkan gen spesifik Leptospira. Sampel urin dan kuesioner diperoleh dari lima belas petugas kebersihan. Sampel dikultur dalam EMJH-broth dengan penambahan 5-fluorouracil, diinkubasi selama 3 bulan dan diamati pertumbuhan bakterinya menggunakan mikroskop medan gelap. Identifikasi bakteri dilakukan dengan PCR, menargetkan gen lipl32, rrl, flaB, ompL1, dilanjutkan dengan sequencing menggunakan metode Sanger, alignment menggunakan ClustalW dan BLAST. Hasil kuesioner menunjukkan bahwa 26,7% responden memiliki faktor risiko tingkat sedang, dan 53,3% responden telah menerapkan pencegahan leptospirosis yang baik. Analisis korelasi Pearson menunjukkan bahwa ada hubungan antara faktor risiko dan pencegahan. Hasil kultur menunjukkan pertumbuhan pada keempat sampel, dan analisis dengan PCR hanya menunjukkan rrl-PCR memiliki amplikon yang diharapkan. Namun hasil sekuensing menunjukkan bahwa amplikon memiliki homogenitas 99% terhadap Pseudomonas stutzeri. Kesimpulannya, Leptospira tidak ditemukan dalam urin petugas kebersihan, mungkin karena penerapan pencegahan leptospirosis yang baik.Kata kunci: Leptospira, Leptospiruria, Petugas Kebersihan, Faktor Risiko

PENDIDIKAN HIV-AIDS DI JOHAR BARU, KEMAYORAN DAN CEMPAKA PUTIH, JAKARTA PUSAT Citra Fitri Agustina; Titiek Djannatun; Dian Widiyanti
Info Abdi Cendekia Vol 2 No 2: Desember 2019
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.266 KB)

Human immunodeficiency Virus (HIV) dan AIDS (Acquired Immunodeficiency Syndrome),adalah penyakit yang disebabkan oleh infeksi virus HIV. Kasus HIV-AIDS di dunia terus mengalamipeningkatan setiap tahunnya. Penyakit ini menjadi masalah kesehatan bagi masyarakat, karena belumtersedianya obat dan vaksin untuk pencegahan. Angka penderita HIV bertambah denganmeningkatnya angka penderita TB (Tuberkulosis) dengan BTA (Basil Tahan Asam) positif.Komorbiditas TB dengan infeksi HIV dapat memengaruhi pengobatannya. Data menunjukkanprevalensi HIV pada insiden kasus TB 6,2%, kematian penderita TB dengan HIV mencapai8,5/100.000 penduduk. Hal ini terjadi karena banyak penderita TB yang tidak mau melakukanpemeriksaan terhadap HIV karena pengetahuan mereka yang kurang tentangnya. Kegiatanpengabdian masyarakat ini bertujuan meningkatkan pengetahuan masyarakat tentang HIV-AIDS. Kesadaran yang tinggi pada masyarakat tentang bahaya infeksi HIV-AIDS memengaruhi masyarakatberobat sehingga dapat melakukan hidup normal dan produktif. Metode yang digunakan adalahmemberikan penyuluhan kepada masyarakat di Kecamatan Johar Baru, Cempaka Putih danKemayoran di Wilayah Jakarta Pusat melalui diskusi interaktif. Terdapat 75 peserta yang mengikutikegiatan dengan kenaikan nilai mencapai 81% di Kecamatan Kemayoran. Hal ini disebabkan olehtingkat pengetahuan awal yang lebih rendah dibandingkan peserta di Cempaka Putih dan Johar BaruEdukasi ini bermanfaat untuk memberikan wawasan mengenai TB-HIV dan HIV-TB, baik kepadapasien, kader, tokoh agama, tokoh masyarakat dan warga.

Hubungan Penggunaan Panty Liner Terhadap Kejadian Leukorrhea Pada Mahasiswi Universitas Yarsi dan Tinjauannya Dalam Agama Islam Kalila Nisya Ramadhan; dian widiyanti; muhammad arsyad
Junior Medical Journal Vol 1, No 4 (2022)
Publisher : Junior Medical Journal

Latar Belakang: Leukorrhea adalah sekret putih dan kental yang keluar dari vagina dan rongga uterus. Di Indonesia sekitar 90% wanita berpotensi mengalami keputihan dikarenakan Indonesia memiliki iklim tropis yang hangat dan lembab, sehingga memudahkan pertumbuhan jamur dan mengakibatkan keputihan. Untuk menghindari menempelnya vaginal discharge di pakaian dalam, banyak wanita, terutama dewasa muda, menggunakan panty liner sehari-hari. Oleh karena itu, penelitian ini bertujuan untuk mengetahui hubungan penggunaan panty liner dengan timbulnya leukorrhea pada dewasa muda. Dalam pandangan Islam cairan keputihan yang keluar dari alat reproduksi wanita merupakan hal yang wajar terjadi pada semua wanita, sehingga dikembalikan ke hukum dasarnya yaitu suci. Metode: Penelitian ini merupakan penelitian analitik kuantitatif dengan pendekatan cross-sectional. Data diambil dengan kuesioner yang disebarkan pada 88 responden mahasiswi Universitas Yarsi. Penetapan sampel dilakukan dengan simple random sampling. Analisis statistik menggunakan uji chi-square dengan SPSS 26. Hasil: Pada penelitian ini didapatkan 85,2% responden mengganti panty liner 3-6 jam sehari. Sebanyak 84,1% responden mengalami leukorrhea normal. didapatkan sebanyak 82,6% responden mengalami jumlah leukorrhea yang sama dan sebanyak 90,2% responden mengalami jumlah yang berkurang seperti sebelum pemakaian. Frekuensi penggantian panty liner memiliki hubungan dengan jumlah dan jenis leukorrhea. Kesimpulan: Frekuensi penggantian panty liner mempengaruhi jumlah dan jenis leukorrhea. Penggantian panty liner yang baik yaitu 3-6 jam sehari menyebabkan leukorrhea yang timbul adalah leukorrhea normal dengan jumlah yang sama atau berkurang seperti sebelum pemakaian.

The Effect Of Learning Stress In The Faculty Of Medicine On The Arising Of Acne Vulgaris In Students Of The Faculty Of Medicine YARSI University And Its Review From The Islamic View Neil Author; Dian Widiyanti; Muhammad Arsyad
Junior Medical Journal Vol 1, No 5 (2023)
Publisher : Junior Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v1i5.3120

Stress is an emotional mental disorder that a person faces because of pressure. Stress is an emotional mental disorder that a person faces because of pressure. Psychosocial stressors can cause physical pain, such as headaches, dizziness, pimples, and so on. The purpose of this research is knowing the effect of stress studying at the Faculty of Medicine with the emergence of acne vulgaris in students of the Faculty of Medicine, YARSI University. The type of research that will be used in this study is the observational analytic method. The population in this study were students of class 2020 and 2021 at the Faculty of Medicine, YARSI University. Determination of the sample to be carried out is by simple random sampling. This study used univariate and bivariate analysis methods. Univariate analysis to describe the characteristics of respondents, the incidence of learning stress and acne vulgaris. The results showed that respondents who experienced academic stress based on the Scale for Assessing Academic Stress questionnaire were 62 students (39.5%). Respondents who suffered from acne vulgaris based on the Global Acne Grading System questionnaire were 133 students in the mild category and 5 students in the moderate category. The results of this study indicate that there is no relationship between stress and the incidence of acne vulgaris in Yasri University Medical Faculty Students Class of 2020-2021. In Islam, everyone will not escape from being given trials by Allah SWT. So in facing trials a Muslim needs to have patience and always think positively.

Hubungan Penggunaan Sabun Pembersih Kewanitaan Terhadap Kejadian Keputihan pada Mahasiswi Universitas Yarsi dan Tinjauannya Menurut Pandangan Islam Fatmah Apriani; Dian Widiyanti; Muhammad Arsyad
Junior Medical Journal Vol 1, No 7 (2023)
Publisher : Junior Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v1i7.3246

Menurut Departemen Kesehatan Indonesia kejadian keputihan banyak dialami oleh para remaja usia produktif, dikarenakan terdapat kebiasaan sejak remaja yang berperilaku buruk dalam menjaga kebersihan organ genetalianya. Kebanyakan wanita menggunakan sabun pembersih kewanitaan untuk memberikan aroma yang wangi, dan juga untuk mencegah terjadinya keputihan. Penelitian ini bertujuan untuk mengetahui hubungan pemakaian sabun pembersih kewanitaan dengan kejadian leukorrhea pada mahasiswi Universitas Yarsi dan tinjauannya dalam agama Islam. Dalam agama Islam leukorrhea atau dikenal sebagai ifrazat hukumnya diperdebatkan antara najis dan juga tidak najis. Metode: Penelitian ini menggunakan metode penelitian analitik kuantitatif dengan pendekatan cross-sectional. Data diambil dengan menggunakan kuesioner yang disebarkan pada 84 responden mahasiswi Universitas Yarsi. Penetapan sampel dilakukan dengan teknik simple random sampling. Analisis statistik penelitian ini menggunakan uji chi-square dengan menggunakan SPSS edisi 26. Hasil: Pada penelitian ini didapatkan hasil 71,4% responden memiliki tingkat pengetahuan mengenai sabun pembersih kewanitaan yang cukup, responden yang tidak menggunakan sabun pembersih kewanitaan setiap bulannya 76,0% mengalami leukorrheafisiologis, 74,1% mahasiswi yang menggunakan sabun pembersih kewanitaan dalam kategori setiap hari mengalami leukorrhea patologis. Setelah menggunakan sabun pembersih kewanitaan dalam kategori sering sebanyak 1-3 kali per bulan dengan leukorrhea yang meningkat berjumlah 70,0%. Terdapat hubungan antara frekuensi pemakaian sabun pembersih kewanitaan dengan kejadian leukorrhea. Kesimpulan: Frekuensi penggunaan sabun pembersih kewanitaan dapat mempengaruhi jenis dan timbulnya leukorrhea, dimana terdapat resiko timbulnya jenis leukorrheapatologis pada mahasiswi yang sering menggunakan sabun pembersih kewanitaan. Dalam agama Islam menjaga kebersihan merupakan hal yang sangat penting yang perlu diperhatikan terutama dalam menjaga alat reproduksi wanita.

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