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All Journal AL KAUNIYAH YARSI Medical Journal ARTERI : Jurnal Ilmu Kesehatan Makara Journal of Health Research Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Razari, Intan
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Electrical & Electronics Engineering Engineering Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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RELATIONSHIP BETWEEN TNF-238G>A POLYMORPHISM AND PREDISPOSITION TO PULMONARY TUBERCULOIS INFECTION IN THE INDONESIAN POPULATION (A PILOT STUDY) Putri, Syurlia; Rasmiyyah, Sausan; Huskany, Evita Amalia; Razari, Intan; Wicaksono, Britanto Dani; Yuliwulandari, Rika
Jurnal Kedokteran YARSI Vol 23, No 1 (2015): JANUARI - APRIL 2015
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v23i1.112

Abstract

Previous studies suggested that genetic factors exerted huge influence in susceptibility to Tuberculosis. Tumor Necrosis Factor Alpha (TNF-a), which is encoded by the TNF gene, play a role on pulmonary macrophage function in isolating and controlling Mycobacterium tuberculosis infection. Polymorphisms on the promoter region of the TNF gene have been predicted to affect its transcriptional activity. Therefore, these polymorphisms are an excellent candidate to further study the role of TNF-a in susceptibility to Tuberculosis. 100 pulmonary tuberculosis patients (case) and 100 healthy individuals (controls) were recruited for this pilot study. DNA samples from cases and controls were genotyped for the TNF -238G A SNP (rs361525) using LightSNip genotyping assay. Our results showed no significant difference in the distribution of TNF -238 genotypes in case and control subjects (P = 0.4335). Further investigation on TNF -238 allele frequencies between case and control studies also yields no significant difference (P=1.000; OR=1.000; %95CI [0.246597 ? 4.055200]) which may suggest that there are no association with predisposition to Tuberculosis infection. In conclusion, this pilot study showed that the TNF -238G A SNP is not associated with susceptibility to Tuberculosis.
Association between rs2787094 Genetic Variants in ADAM33 Gene and Asthma in Indonesian Population: Preliminary study Viyati, Kencono; Prayuni, Kinasih; Zulhamidah, Yenni; Razari, Intan; Yuliwulandari, Rika
Makara Journal of Health Research Vol. 27, No. 2
Publisher : UI Scholars Hub

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Abstract

Background: Asthma is a multifactorial disease that encompasses a multitude of genetic and environmental factors. One such factor is the disintegrin and metalloprotein-33 (ADAM33) gene, which is correlated with asthma and bronchial hyperresponsiveness. Previous studies conducted on Asian populations have reported a significant association between rs2787094 polymorphism in the ADAM33 gene and asthma. Methods: Our study involved 153 Indonesian participants. TaqMan genotyping assay was used to analyze rs2787094 polymorphism in the ADAM33 gene. Results: No significant association was detected between the allele and genotype frequencies of rs2787094 and asthma in the case and control subjects (p = 1.00). The distribution of rs2787094 genotypes in healthy controls was CC (12.1%), CG (42.1%), and GG (45.8%). The genotype distribution in Indonesians was similar to East Asians in 1,000 genomes dataset. Conclusions: This is the first study to investigate the association between rs2787094 polymorphism in the ADAM33 gene and asthma in the Indonesian population and concluded that it is not associated. Future studies with larger sample sizes and more single nucleotide polymorphisms in the ADAM33 gene are needed to validate these results.
Pengembangan Metode PCR Multipleks untuk Analisis Genotipe Null Gen GSTM1/GSTT1 pada Pasien Tuberkulosis Prayuni, Kinasih; Razari, Intan; Nihayah, Silviatun; Syafrizal; Yuliwulandari, Rika
ARTERI : Jurnal Ilmu Kesehatan Vol 4 No 4 (2023): Agustus
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v4i4.289

Abstract

Tuberculosis (TB) remains Indonesia's leading infectious disease. Hepatotoxicity is the most common side effect of TB first-line medication therapy in TB patients. GSTM1 and GSTT1 are glutathione S-transferase (GST) genes involved in the detoxification of various toxic compounds such as drugs. The development of fast and simple methods for null genotyping of GSTM1/GSTT1 could facilitate large pharmacogenetic studies and the clinical application of personalized drug dose adjustment according to the patient's genetic profile. The aim of this research was to develop a multiple PCR method for simultaneous amplification of GSTM1/GSTT1 genes for molecular analysis. A total of 25 samples of TB patients were used to validate the method consisting of TB patients with hepatotoxicity and without hepatotoxicity. Our result showed the genotype frequency of the GSTM1 null genotype was 90% in TB patients with hepatotoxicity and 100% in TB patients without hepatotoxicity. The frequency of the GSTT1 null genotype in TB patients with hepatotoxicity was 90%, whereas in TB patients without hepatotoxicity was 80%. The sequencing results on the positive samples showed a similarity of 99% to the GenBank NCBI. Our study was successful in detecting GSTM1 and GSTT1 null genotypes using the multiplex PCR method in TB patients. Further study needs to be done with larger sample of TB patients.
Identifikasi DNA Ikan Sapu-sapu (Pterygoplichthys sp.) Pada Siomai dengan DNA Barcoding Suseno, Dedy; Razari, Intan
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.34277

Abstract

AbstrakSiomai adalah salah satu jenis makanan yang menggunakan bahan baku utama ikan. Pada dasarnya pembuatan siomai dapat menggunakan berbagai jenis ikan, ikan sapu-sapu bisa jadi salah satunya. Ikan sapu-sapu merupakan salah satu jenis ikan yang banyak hidup di perairan Indonesia misalnya pada Sungai Ciliwung. Tercemarnya air Sungai Ciliwung dengan logam berat Hg, Pb, Cd, dan Ag sangat memungkinkan logam-logam berat tersebut terakumulasi pada ikan sapu-sapu. Tujuan penelitian ini yaitu mengidentifikasi DNA ikan sapu-sapu pada sampel siomai dan menganalisis adanya kandungan logam berat pada sampel siomai yang mengandung ikan sapu-sapu. Sampel siomai yang dianalisis sebanyak 28 sampel. Sampel ini didapatkan dari 28 lokasi berbeda di sekitar stasiun kereta api Provinsi DKI Jakarta. Analisis DNA ikan sapu-sapu menggunakan primer spesifik dan analisis logam berat menggunakan metode Atomic Absorption Spectrophotometry (AAS). Sebanyak 9 dari 28 sampel memperlihatkan pita berukuran 496 pb pada gel elektroforesis. Pita tersebut terkonfirmasi spesies ikan sapu-sapu (Pterygoplichthys pardalis dan Hypostomus plecostomus) setelah dianalisis menggunakan sequencing sanger. Sebanyak 9 sampel yang teridentifikasi mengandung ikan sapu-sapu juga mengandung logam berat Pb dengan rentang konsentrasi 0,06 mg/kg sampai 0,12 mg/kg dan logam berat Cd <0,007 mg/kg. Kandungan logam berat pada sampel siomai masih di bawah ambang batas aman sesuai ketentuan SNI dan BPOM.AbstractSiomai is a type of food that uses fish as the main ingredient. Siomai can be made by using various types of fish, suckermouth catfish can be one of them. Suckermouth catfish is a type of fish that lives a lot in Indonesian rivers, for example in the Ciliwung River. Pollution of Ciliwung River water with heavy metals Hg, Pb, Cd, and Ag makes it possible for these heavy metals to accumulate in the suckermouth catfish. The purpose of this study is to identify the suckermouth catfish DNA in the siomai sample and analyze the presence of heavy metals in the siomai sample containing suckermouth catfish. Siomai samples analyzed were 28 samples. This sample was obtained from 28 different locations around the DKI Jakarta Provincial train station. DNA Analysis of the suckermouth catfish using specific primers and analysis of heavy metals using the Atomic Absorption Spectrophotometry (AAS) method. Nine of the 28 samples showed a 496 bp band on the electrophoretic gel. The band was confirmed by the species of suckermouth catfish (Pterygoplichthys pardalis and Hypostomus plecostomus) after being analyzed using Sanger sequencing. A total of 9 samples that were identified as containing suckermouth catfish also contained the heavy metal Pb with a concentration range of 0.06 mg/kg to 0.12 mg/kg and the heavy metal Cd <0.007 mg/kg. The heavy metals content in the siomai sample is still below the safe threshold according to SNI and BPOM provisions.
Effect of Green Tea Extract to the Degree of Knee Joint Damage and Nitric Oxide Levels in the Rabbit Osteoarthitis Model Susmiarsih, Tri Panjiasih; Hadi, Restu Syamsul; Sofwan, Achmad; Kuslestari, K; Razari, Intan
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2019: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.1438

Abstract

Osteoathritis (OA) is characterized by degeneration of articular cartilage, subchondral bone, synovial fluid and synovium. Nitric oxide (NO) is proinflammatory cytokine that play a significant role in the pathogenesis of OA via cartilage and bone degradation by synovial inflammation. Green tea is a novel functional food for treating osteoarthritis and inhibiting the production of inflammatory mediators such as nitric oxide. This study aimed to evaluate the effects of green tea extract to the nitric oxide levels and degree of knee joint damage in the rabbit osteoarthitis model. The Freud’s adjuvant complete was performed to induce OA, as many as sixteen male rabbits (New Zealand white) were randomly divided into four groups: adjuvant injection, adjuvant and green tea (injection), adjuvant and green tea (per oral), and control group. The control group only received drinking water, the Freud adjuvant (0.2 ml) and green tea extract (200 mg/kg bw) were orally and injection administered for eight weeks. The articular cartilage damage was evaluated histologically according to MANKIN score. NO levels were determined by nitric oxide assay. Data was analysed by Chi square test. The result of this study showed the surface structure damage of cartilage increased after adjuvant-induced. Green tea extract decrease significantly (p=0.02) the degree of knee joint damage after adjuvant-induced in rabbit osteoarthritis models. NO levels increased after OA induction. The green tea extract administration (via injection) can significantly (p = 0.038) decrease NO levels compare to adjuvant group. Green tea extract decrease the knee joint damage and NO proinflamantory levels in rabbit of osteoarthritis model
Co-Authors Britanto Dani Wicaksono, Britanto Dani Huskany, Evita Amalia Kinasih Prayuni, Kinasih Kuslestari, K Nihayah, Silviatun Putri, Syurlia Rasmiyyah, Sausan Restu Syamsul Hadi Rika Yuliwulandari, Rika Sofwan, Achmad Suseno, Dedy syafrizal Tri Panjiasih Susmiarsih Viyati, Kencono Yenni Zulhamidah, Yenni