Article Per Year (5 Year)
p-Index From 2020 - 2025
0.562
P-Index
This Author published in this journals
All Journal Majalah Kedokteran Bandung Indonesian Journal of Tropical and Infectious Disease Majalah Sainstekes ARTERI : Jurnal Ilmu Kesehatan Makara Journal of Health Research
Kinasih Prayuni, Kinasih
Departemen Farmakologi, Fakultas Kedokteran, Universitas YARSI Jakarta,
Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Pengembangan Metode In-House HLA-Typing Gen HLA Kelas I (HLA A, HLA B, dan HLA C) Menggunakan Next Generation Sequencing Illumina MiSeq Yuliwulandari, Rika; Prayuni, Kinasih; Kenconoviyati, Kenconoviyati; Susilowati, R. W.; M. Sofro, Abdul Salam
Majalah Kedokteran Bandung Vol 47, No 3 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Human leucocyte antigen (HLA) adalah protein penyaji antigen yang lokus genetiknya berada di kromosom 6p21 dengan ukuran sebesar 3,8 Mb dan berasosiasi dengan lebih dari 100 penyakit berbeda yang  kebanyakan merupakan penyakit autoimun. Proses HLA-typing menggunakan sekuensing Sanger masih memberikan ambiguitas terhadap determinasi alel, low-throughput, dan membutuhkan biaya besar untuk sampel dalam jumlah besar. Next generation sequencing (NGS) menjadi metode yang dapat mengatasi kelemahan sekuensing Sanger. MiSeq dari Illumina merupakan salah satu NGS yang digunakan untuk HLA-typing. MiSeq memberikan kemudahan preparasi dan fleksibilitas metode yang dapat dikembangkan sesuai dengan kebutuhan laboratorium penelitian. Penelitian dilakukan di Laboratorium Molekular Genetik, Laboratorium Terpadu Universitas YARSI pada empat orang mahasiswa Fakultas Kedokteran Universitas YARSI etnik Melayu selama periode Mei–Desember 2014. Hasil menunjukkan terdapat total 546 SNP heterozygous, 888 SNP homozygous, 25 insersi, dan 23 delesi dari keseluruhan 11 sampel amplikon dengan coverage 2.106,536x dengan 2x25 siklus pembacaan. Optimasi metode HLA-typing dapat dikatakan berhasil dengan mengombinasikan long-range PCR dan pemilihan ukuran library 300–600 bp. [MKB. 2015;47(3):152–159]Kata kunci: HLA Kelas I, MiSeq, next generation sequencingDevelopment of Class I HLA Gene In-House HLA-Typing Methods (HLA A, HLA B, and HLA C) using Next Generation Sequencing Illumina MiSeqAbstractHuman leukocyte antigen (HLA) is a 3.8 Mb protein presenting antigen whose genetic locus is located in chromosome 6p21 area and have association with more than 100 different diseases that are mostly autoimmune diseases. HLA-typing process using Sanger sequencing still  creates ambiguity in the  determination of  alleles, low-throughput, and costly as it requires a large quantity of sample. Next generation sequencing (NGS) is a method that can overcome the drawbacks of Sanger sequencing. MiSeq Illumina is one of the NGSs that are used for HLA-typing. This study was conducted at the Laboratory of Molecular, Universitas YARSI in a period from May to December 2014. MiSeq provides convenience and flexibility in the preparation methods that can be developed according to the needs of the research laboratory. The results showed that there were a total of 546 SNPs that were heterozygous, 888homozygous SNP, 25 insertions and 23 deletions from the overall 11 amplicon samples with an average coverage with 2x25 read length of  2,106,536x. Our protocol generates good result as we combined long PCR amplicon and size selection method to 300–600 bp fragment.  [MKB. 2015;47(3):152–159]Key words: HLA Class I, MiSeq, next generation sequencing DOI: 10.15395/mkb.v47n3.389
Association between rs2787094 Genetic Variants in ADAM33 Gene and Asthma in Indonesian Population: Preliminary study Viyati, Kencono; Prayuni, Kinasih; Zulhamidah, Yenni; Razari, Intan; Yuliwulandari, Rika
Makara Journal of Health Research
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Asthma is a multifactorial disease that encompasses a multitude of genetic and environmental factors. One such factor is the disintegrin and metalloprotein-33 (ADAM33) gene, which is correlated with asthma and bronchial hyperresponsiveness. Previous studies conducted on Asian populations have reported a significant association between rs2787094 polymorphism in the ADAM33 gene and asthma. Methods: Our study involved 153 Indonesian participants. TaqMan genotyping assay was used to analyze rs2787094 polymorphism in the ADAM33 gene. Results: No significant association was detected between the allele and genotype frequencies of rs2787094 and asthma in the case and control subjects (p = 1.00). The distribution of rs2787094 genotypes in healthy controls was CC (12.1%), CG (42.1%), and GG (45.8%). The genotype distribution in Indonesians was similar to East Asians in 1,000 genomes dataset. Conclusions: This is the first study to investigate the association between rs2787094 polymorphism in the ADAM33 gene and asthma in the Indonesian population and concluded that it is not associated. Future studies with larger sample sizes and more single nucleotide polymorphisms in the ADAM33 gene are needed to validate these results.
Detection of ADAM33 Gene Variants Using Sanger Sequencing Suwardji, Kencono Viyati; Prayuni, Kinasih; Andayani, Sri Hastuti; Zulhamidah, Yenni; Sofwan, Achmad; Muflihah, Lilah; Yunus, Ronike; Junaefi, Junaefi; Judasah, Ijun
Majalah Sainstekes Vol. 10 No. 2 (2023): DESEMBER 2023
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/ms.v10i2.3962

Abstract

Asma adalah penyakit pernafasan yang ditandai oleh obstruksi saluran napas yang disebabkan oleh peradangan bronkus akut dan kronis. Gen disintegrin and metalloprotease 33 (ADAM33) merupakan gen terkait kerentanan terhadap asma dan diketahui memiliki lebih dari 300 polimorfisme. Studi meta-analisis melaporkan bahwa rs2280091, rs2787094, rs511898, rs2280089 dan rs2280090 memiliki asosiasi kuat dengan asma pada populasi Asia. Penelitian pendahuluan ini mengumpulkan 10 partisipan dengan penyakit asma dan 10 partisipan sehat untuk mengidentifikasi alel dan genotipe dari masing-masing polimorfisme menggunakan metode amplifikasi dan sekuensing Sanger. Hasil sekuensing menunjukkan bahwa 60% dari sampel yang diuji memiliki genotipe heterozigot untuk rs2280090 dan rs228091 baik pada sampel kasus maupun kontrol. Varian SNP rs2280096 menunjukkan bahwa frekuensi alel homozigot wildtype sebesar 60% baik pada sampel kasus maupun kontrol. Penelitian lanjutan perlu dilakukan untuk mengetahui apakah varian SNP tersebut memiliki aosisasi yang positif dengan penyakit asma.
THE RISK FACTORS FOR DRUG INDUCED HEPATITIS IN PULMONARY TUBERCULOSIS PATIENTS IN DR. SOETOMO HOSPITAL Soedarsono, Soedarsono; Mandayani, Sari; Prayuni, Kinasih; Yuliwulandari, Rika
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 3 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.097 KB) | DOI: 10.20473/ijtid.v7i3.8689

Abstract

Tuberculosis (TB) is still a major public health problem in Indonesia. Anti-tuberculosis drug-induced hepatotoxicity (DIH) is common side effect leading to changes in treatment regimens, and the less effective second-line treatments. Several risk factors such as age, sex, body mass index (BMI) and acetylization status for hepatotoxicity were suggested in previous studies but in the fact, those are often not related to DIH incidence after receiving standard TB treatment regimen. The aim of this study was to asses the role of risk factors in the DIH incidence in pulmonary TB patients receiving standard TB treatment regimen in Dr. Soetomo Hospital, Surabaya. Study design was analytic observational with case control. The subjects were 30 TB DIH patients and 31 TB non-DIH patients receiving standard national TB program therapy. DIH severity was divided based on International DIH Expert Working Group. Demographic data and BMI status were taken from medical records. The age classification are ≥35 years old and <35 years old as one of the risk factors studied. DNA sequencing was used to assess single-nucleotide polymorphisms in NAT2 coding region to evaluate acetylator status from blood samples. The risk factors were evaluated using chi-square test and Mantel-Haenszel test. Significant association between low BMI and DIH in general was identified (OR=3.017; 95% CI=1.029-8.845) and more significant association between low BMI and moderate DIH (OR=15.833; 95% CI=1.792-139.922). Age, sex, and acetylization status has no significant correlation with DIH incidence in general. Significant association between slow acetylator phenotype and incidence of moderate DIH was identified (OR=7.125; 95% CI= 1.309-38.711). In conclusion, some risk factors were correlated to DIH incidence in pulmonary TB patientsreceiving standart TB treatment regimen.
Pengembangan Metode PCR Multipleks untuk Analisis Genotipe Null Gen GSTM1/GSTT1 pada Pasien Tuberkulosis Prayuni, Kinasih; Razari, Intan; Nihayah, Silviatun; Syafrizal; Yuliwulandari, Rika
ARTERI : Jurnal Ilmu Kesehatan Vol 4 No 4 (2023): Agustus
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v4i4.289

Abstract

Tuberculosis (TB) remains Indonesia's leading infectious disease. Hepatotoxicity is the most common side effect of TB first-line medication therapy in TB patients. GSTM1 and GSTT1 are glutathione S-transferase (GST) genes involved in the detoxification of various toxic compounds such as drugs. The development of fast and simple methods for null genotyping of GSTM1/GSTT1 could facilitate large pharmacogenetic studies and the clinical application of personalized drug dose adjustment according to the patient's genetic profile. The aim of this research was to develop a multiple PCR method for simultaneous amplification of GSTM1/GSTT1 genes for molecular analysis. A total of 25 samples of TB patients were used to validate the method consisting of TB patients with hepatotoxicity and without hepatotoxicity. Our result showed the genotype frequency of the GSTM1 null genotype was 90% in TB patients with hepatotoxicity and 100% in TB patients without hepatotoxicity. The frequency of the GSTT1 null genotype in TB patients with hepatotoxicity was 90%, whereas in TB patients without hepatotoxicity was 80%. The sequencing results on the positive samples showed a similarity of 99% to the GenBank NCBI. Our study was successful in detecting GSTM1 and GSTT1 null genotypes using the multiplex PCR method in TB patients. Further study needs to be done with larger sample of TB patients.
Co-Authors Abdul Salam M. Sofro, Abdul Salam Andayani, Sri Hastuti Judasah, Ijun Junaefi, Junaefi Kenconoviyati Kenconoviyati, Kenconoviyati Mandayani, Sari Muflihah, Lilah Nihayah, Silviatun R. W. Susilowati, R. W. Razari, Intan Rika Yuliwulandari, Rika Soedarsono Soedarsono Sofwan, Achmad Suwardji, Kencono Viyati syafrizal Viyati, Kencono Yenni Zulhamidah, Yenni Yunus, Ronike