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Pemeriksaan Jamur Candida albicans pada Urine Penderita Diabetes Melitus di Rumah Sakit Umum Islam Klaten Purwitaningsih, Ani; Setya, Adhi Kumoro
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 9 No. 1 (2023): Anakes: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v9i1.898

Diabetes melitus saat ini telah menjadi menjadi ancaman serius kesehatan global. Berdasarkan dataÂ International Diabetes FederationÂ (IDF), Indonesia berstatus waspada diabetes karena menempati urutan ke tujuh dari sepuluh negara dengan jumlah pasien diabetes tertinggi. Diabetes melitus dapat menyebabkan komplikasi penyakit baik akut maupun kronis dan menyebabkan daya tahan tubuh menurun sehingga pasien mudah terserang infeksi. Indonesia beriklim tropis dengan kelembaban tinggi, daerah genetalia pada pasien diabetes melitus banyak mengandung glukosa yang merupakan nutrisi pertumbuhan jamur, sehingga pasien diabetes melitus mudah terserang infeksi jamur. Jamur yang paling banyak menyebabkan infeksi adalahÂ Candida albicans. Penelitian ini bertujuan untuk memeriksa pertumbuhan jamurÂ Candida albicansÂ pada urine penderita diabetes mellitus di Rumah Sakit Umum Islam Klaten dengan menggunakan mediaÂ CHROM agar CandidaÂ (CAC) dan menghitung jumlah koloninya. Hasil penelitian didapatkan dari 30 sampel urine penderita diabetes melitus di Rumah Sakit Umum Islam Klaten ada 6 sampel yang positif tumbuhÂ Candida albicansÂ (20%) dengan jumlah koloni paling banyak 1470 koloni dan paling sedikit 60 koloni. Dari hasil penelitian dapat disimpulkan ada pertumbuhanÂ Candida albicansÂ pada urine penderita diabetes melitus.Kata kunci : Diabetes melitus,Â Candida albicans,Â CHROM agar CandidaÂ (CAC)Kata kunci : Diabetes melitus, Candida albicans, CHROMÂ agar CandidaÂ (CAC)

POTENSI BIJI MENGKUDU (Morinda citrifolia L.) SEBAGAI ANTELMINTIK TERHADAP Ascaris suum Setya, Adhi Kumoro; Suwarmi, Sri
Media Farmasi Indonesia Vol. 13 No. 1 (2018): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (660.378 KB)

Abstract Parasitic infection from Genus Ascaris (Ascaris suum and Ascaris lumbricoides) is the parasite that most frequently causes worms in humans and livestock. This infection can cause intellectual retardation, cognitive and educational deficits with impacts on the nation productivity espesialy in children. It is known that a number of harmful synthetic drugs have side effects to health. In decades, mengkudu (Morinda citrifolia) is known to be useful in pharmacology to overcome health problems. In this fruit, there are seeds that have not been widely studied despite its benefits, and oftentimes, these seeds only go as waste product. The present research is aimed to show whether mengkudu seeds are potential to be exploited as antelmintic. The antelmintic ability of mengkudu seed extract was measured using regretion-probit analysis, and its pathological effects were tested through histologic painting. This research used five variations of concentration with three replicates. The worms per treatment were 4. The analysis result showed that lethal concentration (LC50) of 7.12% and lethal time (LT50) of 174 minutes. The histology of worms’ intestines demonstrates intestinal microfili that they become dull and their neural networks become tenuous. We report that mengkudu seed extract has antelmintic potency.

Lilin Dari Kulit Lemon (Citrus limon) Pencegah Demam Berdarah Di Desa Mayang Sukoharjo Setya, Adhi Kumoro; Haryatmi, Dwi; Asmarani, Dewi Prantiannita Putri; Margareta, Rany Gina; Noviyanti, Mike; Waritsini, Belinda Khoirul
Mestaka: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 1 (2025): Februari 2025
Publisher : Pakis Journal Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58184/mestaka.v4i1.608

Every year dengue fever cases occur in Sukoharjo District. One of them occurred in Gatak Sub-district, where previously 3 residents were infected with the disease. There is one area, namely Mayang Village, where cadres and several residents cultivate lemons. In lemons, there is a skin that is often ignored but has potential that not many people are aware of, such as; preventing nausea, therapy and anti-insects, especially mosquitoes. One of the active substances contained in lemon peel, namely citronella, produces an aroma that insects do not like. The purpose of this community service is; providing knowledge about dengue fever, utilizing lemon peel into a health product, namely mosquito repellent aromatherapy candles and eliminating dengue fever. This community service activity was delivered using a counseling method equipped with a video screening of dengue fever and a demonstration of making mosquito repellent aromatherapy candles from lemons cultivated by the surrounding community. This activity was attended by 20 cadres from an initial target of 30 people. Participants were enthusiastic about the 7 questions presented, and in the results of the initial knowledge test and after the activity there was an increase in the participants' knowledge scores. In this activity, mosquito repellent aromatherapy candle products were produced which can be made or further developed into sales products.

Prevalence of Trichomoniasis in Women with HIV in Sragen District Setiowati, Hanny Endah; setya, Adhi Kumoro
Indonesian Journal of Global Health Research Vol 7 No 3 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i3.5954

Trichomonas vaginalis infection remains one of the most common non-viral sexually transmitted infections (STIs) globally, despite being one of the most easily treatable STIs. This study aims to determine the prevalence and risk factors associated with trichomoniasis among HIV-positive women in Sragen Regency. A total of 30 HIV-diagnosed women from the Sehat Panghuripan Sukowati Foundation in Sragen, aged 15–49 years, participated in this study. Respondents completed a questionnaire and underwent urinalysis at Prodia Medical Laboratory, Sragen. The presence of Trichomonas vaginalis infection was evaluated using a wet mount microscopic method. Clinical features were collected through structured questionnaires administered to each participant. Result: The findings revealed the prevalence of Trichomoniasis among HIV-positive women, indicating that Trichomoniasis can increase HIV viral load in vaginal fluid, potentially heightening the risk of HIV transmission. The study demonstrated a higher infection rate in women with a history of sexually transmitted infections (HIV), particularly those over 30 years of age. Most participants were housewives with a single sexual partner, reported no vaginal discharge complaints, but had experienced abnormal vaginal secretions in the past. The recorded prevalence of Trichomoniasis in this study was 10% (3 out of 30 samples)

Penyuluhan dan Pemanfaatan Barang Bekas menjadi Ovitrap untuk Mengendalikan Populasi Aedes Aegypti di Langenharjo Grogol Sukoharjo Setya, Adhi Kumoro; Wahyudi, Didik; Kusuma, Eka Wisnu; Agung, Rendyansah; Widyastuti, Dinda; Valent, Febriana
Mestaka: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 4 (2025): Agustus 2025
Publisher : Pakis Journal Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58184/mestaka.v4i4.747

Since its first discovery in Indonesia, dengue fever (DHF) has remained a public health threat. Numerous measures have been taken to break the life cycle of its vector, Aedes aegypti, but the number of cases has yet to be eliminated. The disease seems to spread randomly, and its incidence is unpredictable. Often, outbreaks are accompanied by fatalities. Research into environmentally friendly and safe materials has been extensively conducted, but this disease has not yet been effectively eradicated. To maximize the prevention and elimination of dengue fever, it is crucial to understand the behavior of the vector to achieve accurate and optimal results. The primary objective of this activity is to prevent dengue fever, as several cases of dengue fever were reported in Grogol district earlier this year. This activity was held in May 2024 in Langenharjo sub-district, Sukoharjo city, with 47 participants. After participating, community understanding increased, as reflected in the average score of 89.79. Participants and cadres can carry out and socialize effective dengue fever prevention and are able to prevent and eliminate dengue fever using the ovitrap technique.

Incidence Rates of Pediculosis Capitis and Helminthiasis in Children in Danukusuman Surakarta Setya, Adhi Kumoro; Haryatmi, Dwi; Listiyaningsih, Alfina; Putri, Elisa
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6956

Pediculosis capitis and helminthiasis It is categorized as a neglected disease but is still an important health problem, especially for children. These two diseases each cause clinical disorders on the surface and internal organs of the body. Danukusuman is one of the most densely populated areas in the city of Surakarta, where in previous years many cases of gastrointestinal disorders affected local residents. The aim of this study to determine the incidence of pediculosis capitis and helminthiasis in elementary school children in the city of Surakarta. This research uses a descriptive analysis research design. The population in the study were children aged 6-8 years in Danukusuman Surakarta totaling 100 respondents. Samples were taken using total sampling technique. The diagnosis of pediculosis capitis is made on the skin and hair area of the head and intensity helminth enforced use method Kato katz from fecal samples by calculating the number of worm parasite eggs per gram of feces (EPG). Based on research conducted on 100 respondents, the results showed that 29 (29%) respondents had pediculosis capitis and cases of helminthiasis showed that 2 respondents (2%) had Ascari lumbricoides and Trichuris trichiura with a total of 24 epg of parasites each with a light infection intensity. From the results of this study, it was reported that the incidence of pediculosis capitis in children in Danukusuman was 29% and helminth infections were 2% of cases with a mild degree of infection.

Prevalence of Pediculosis Capitis in Orphanages and Islamic Boarding Schools in the Surakarta Setya, Adhi Kumoro; Haryatmi, Dwi
Indonesian Journal of Global Health Research Vol 5 No 4 (2023): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v5i4.2484

Residential environments such as orphanages and identical boarding houses have quite high population densities. This makes cottages and orphanages vulnerable and has a high potential for transmission of Pediculus Humanus var. capitis causes the disease Pediculosis capitis. This disease is an indicator of poor personal hygiene and the environment in which one lives. The higher the case of tick infestation, the worse the surrounding community becomes. This study aims to determine the prevalence of cases of pediculosis capitis in Islamic boarding schools and orphanages in the Surakarta.This research uses a descriptive research design with purposive sampling technique. The samples analyzed were the hair and scalp areas of 155 child and adolescent respondents in Islamic boarding schools and orphanages in Surakarta City. The data obtained was carried out by laboratory examination in the form of egg, nymph and adult flea stages as well as questionnaires filled out by respondents using guided interviews in the form of data on respondents' characteristics and personal hygiene status obtained through scoring. The relationship between Personal Hygiene and the incidence of Pediculosis capitis was analyzed using the chi-square test. From the results of research conducted on all respondents who were willing and met the criteria, it was discovered that of the 155 respondents, 59 (38%) were positive for pediculosis capitis and 96 (62%) did not experience pediculosis capitis. Sequentially, the highest cases were found in locations; Laweyan sub-district orphanage (76.7%), city orphanage (60%), Pasar Kliwon Islamic boarding school (36%) and Laweyan orphanage (0%). The personal hygiene status of the respondents in this case was 81% good, while 19% had poor personal hygiene status. In all cases that were found to be positive for pediculosis capitis, all of them were found to be in the egg, nymph and adult stages. The highest characteristics of positive cases were found in respondents with straight hair and longer than shoulder length. From this research, it was concluded that there was a significant relationship between personal hygiene and the cases of pediculosis capitis found. The point prevalence of pediculosis capitis in Islamic boarding schools and orphanages in the city of Surakarta is 38% of cases. As many as 81% of respondents had good personal hygiene status and 19% of respondents had poor personal hygiene.

Optimisation of 3% Giemsa Staining Time in Malaria Microscopic Examination Hadiana, Resa Cristina Lilik; Setya, Adhi Kumoro
Indonesian Journal of Global Health Research Vol 7 No 3 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i3.6229

Malaria infection is a significant health issue in Indonesia, with a high prevalence in endemic areas such as Puskesmas Hanura Teluk Pandan. Giemsa 3% staining is used as the standard method for microscopic diagnosis of malaria; however, the duration of staining can affect laboratory efficiency. This study aims to optimize the staining time of Giemsa 3% in microscopic malaria diagnosis to improve efficiency without compromising diagnostic quality. The study used an experimental design with staining time variations of 30 minutes, 40 minutes, and 50 minutes on thin blood smear samples from suspected malaria patients. Staining quality was assessed using a scoring system, with a score of 1 indicating optimal staining (clear background, blue cytoplasm, and red nucleus) and a score of 0 indicating suboptimal staining (unclear background, cytoplasm, and nucleus not stained or not clearly visible). The results showed that all staining time variations produced optimal staining quality (score 1), with no samples receiving a score of 0. The data were found to be non-homogeneous and non-normally distributed, thus the Kruskal-Wallis test was employed for subsequent analysis. The Kruskal-Wallis test showed no significant differences between the three staining time variations (p = 1.000). The conclusion of this study is that staining durations of 30 minutes, 40 minutes, and 50 minutes with Giemsa 3% can produce equivalent staining quality, allowing operational efficiency without compromising the accuracy of malaria diagnosis.

Pemeriksaan Jamur Candida albicans pada Urine Penderita Diabetes Melitus di Rumah Sakit Umum Islam Klaten Purwitaningsih, Ani; Setya, Adhi Kumoro
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 9 No. 1 (2023): Anakes: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v9i1.898

Diabetes melitus saat ini telah menjadi menjadi ancaman serius kesehatan global. Berdasarkan dataÂ International Diabetes FederationÂ (IDF), Indonesia berstatus waspada diabetes karena menempati urutan ke tujuh dari sepuluh negara dengan jumlah pasien diabetes tertinggi. Diabetes melitus dapat menyebabkan komplikasi penyakit baik akut maupun kronis dan menyebabkan daya tahan tubuh menurun sehingga pasien mudah terserang infeksi. Indonesia beriklim tropis dengan kelembaban tinggi, daerah genetalia pada pasien diabetes melitus banyak mengandung glukosa yang merupakan nutrisi pertumbuhan jamur, sehingga pasien diabetes melitus mudah terserang infeksi jamur. Jamur yang paling banyak menyebabkan infeksi adalahÂ Candida albicans. Penelitian ini bertujuan untuk memeriksa pertumbuhan jamurÂ Candida albicansÂ pada urine penderita diabetes mellitus di Rumah Sakit Umum Islam Klaten dengan menggunakan mediaÂ CHROM agar CandidaÂ (CAC) dan menghitung jumlah koloninya. Hasil penelitian didapatkan dari 30 sampel urine penderita diabetes melitus di Rumah Sakit Umum Islam Klaten ada 6 sampel yang positif tumbuhÂ Candida albicansÂ (20%) dengan jumlah koloni paling banyak 1470 koloni dan paling sedikit 60 koloni. Dari hasil penelitian dapat disimpulkan ada pertumbuhanÂ Candida albicansÂ pada urine penderita diabetes melitus.Kata kunci : Diabetes melitus,Â Candida albicans,Â CHROM agar CandidaÂ (CAC)Kata kunci : Diabetes melitus, Candida albicans, CHROMÂ agar CandidaÂ (CAC)

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