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Da Cunha, Theo
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

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Analisis Kandungan Metabolit Sekunder dan Uji aktivitas Antibakteri Jamur Endofit Batang Binahong (Anredera COrdifiolia Steenis) Ola, Antonius R B; Bria, Elisabeth Indriyani Paula; da Cunha, Theo
Chemistry Notes Vol 5 No 2 (2023): Chem Notes, 5(2), 2023
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/cn.v1i2.1197

Abstract

The isolation of endophytic fungi (penicillium sp) from binahong stem had been undertaken with the objective to determine its content of secondary metabolites and its antibacterial activity. The content of secondary metabolites was analyzed with HPLC and its antibacterial activity was measured using paper disc diffusion method. The results showed that there were four compounds in the HPLC chromatogram detected at 235 nm. The antibacterial activity for methanol and ethyl acetate extracts were observed for its diameter of zone inhibition at 18.2 mm and 16.7 mm for S.aureus and 17.8 mm and 16.3 mm for E. Coli.
PENURUNAN KADAR LOGAM KADMIUM PADA UDANG (Peanaeus merguiensis) DENGAN PERENDAMAN CUKA NIRA ENAU (Arenga pinnata) Manu, Rolin M. P.; Tambaru, David; da Cunha, Theo
Chemistry Notes Vol 1 No 1 (2018): Chem. Notes 2018, 1(1)
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/cn.v1i1.1570

Abstract

A research on the decrease of cadmium (Cd) content in shrimps by soaking them in palm (Arenga Pinnata) vinegar has been done. This study aimed to determine the removal percentage (%) of cadmium from shrimp after immersed in palm (Arenga Pinnata) vinegar and to determine the concentration and immersion time optimum in reducing the cadmium metal content in shrimps. The research was conducted in several steps namely sample preparation, determination of optimum concentration of vinegar, simulation of shrimp pollution, sample soaking in palm (Arenga Pinnata) vinegar and determination of cadmium content in samples. A two-way ANOVA test (α = 0,05) showed that the variation of palm (Arenga Pinnata) vinegar concentration has significant effect on reducing Cd content, while contact times have no significant effect on the reduction of Cd content in shrimps. It was found that the optimum concentration of palm (Arenga Pinnata) vinegar was 2% and optimum immersion time was 30 minutes. The removal percentage (%) of cadmium content from shrimps using palm vinegar reached 98.84%.
Purification of Timor’s Genoak (Acorus calamus ) Essential Oil using Reduced Pressure Distillation Henci, Rensy A; da Cunha, Theo; Ishaq Lerrick, Reinner
Chemistry Notes Vol 1 No 1 (2019): Chem. Notes 2019, 1(1)
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/cn.v1i1.1606

Abstract

A research about Asaron oil purification produced over Stahl distillation has been done. This research was conducted by distillation of Genoak’s roots, rhizomes and leaves whilst the purification was carried out through reduced pressure distillation. A yellowish oil was yield respectively 1,83 gram from the roots and rhizomes and 1,26 gram from leaves which then was revealed as 90,49% pure of asaron confirmed using GC-MS, 1H-NMR and FT-IR analysis.
UJI TOKSISITAS EKSTRAK AIR DAUN KELOR (Moringan oleifera, L) ASAL LAHAN KERING NUSA TENGGARA TIMUR da Cunha, Theo; Darmakusuma, Dodi; Ola, Antonius R.B.; Lulan, Theodore Y.K.
Chemistry Notes Vol 7 No 1 (2025): Chem. Notes, 7(1), 2025
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/cn.v7i1.22611

Abstract

Tumbuhan Kelor (Moringa oleifera Lamk) banyak tumbuh di Nusa Tenggara Timur (NTT) yang beriklim Lahan Kering. Daun Kelor banyak digunakan masyarakat NTT sebagai bahan makanan. Daun kelor mempunyai kandungan gizi yang tersebar mulai dari buah, sampai akarnya. Selain itu kelor mempunyai manfaat lain seperti koagulan, vitamin dan sebagai obat. Penelitian ini mempelajari efek toksik ekstrak air daun kelor terhadap larva udang Artemia Salina Leach dengan menggunakan metode BST. Metode ini sebagai pengujian awal dari efek anti bakteri dan anti kanker, penggunakan air (suhu kamar) sebagai pelarut agar dapat diaplikasikan secara langsung oleh masyarakat. Daun kelor dimaserasi selama 3 x 24 jam menggunakan air, hasil uji fitokimia teridentifikasi kandungan metabolic sekunder antara lain, Alkaloid, Flavonoid, Triterpen, dan Tanin. Hasil uji BSLT menunjukan bahwa ekstrak air (suhu kamar) daun kelor mempunyai tingkat toksisitas terhaap Artemia Salina Leach yang ditunjukan dengan nilai LC50 888, 34 kurang dari 1000 ppm. Dapat disimpulkan bahwa daun kelor mempunyai potensi sebagai tanaman yang nantinya dapat dimanfaatkan sebagai anti bakteri dan anti kanker.
Co-Authors Antonius R.B. Ola Bria, Elisabeth Indriyani Paula David Tambaru Dodi Darmakusuma, Dodi Henci, Rensy A Ishaq Lerrick, Reinner Lulan, Theodore Y.K. Manu, Rolin M. P.