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All Journal SIMBIOSA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Ihsan, Kharisul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions

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BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS Savitri, Lisa; Kasimo, Elfred Rinaldo; Ihsan, Kharisul; Faizal, Imam Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10708

Abstract

Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues.
BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS Savitri, Lisa; Kasimo, Elfred Rinaldo; Ihsan, Kharisul; Faizal, Imam Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10708

Abstract

Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues.
Analisis Kekerabatan Populasi Drosophila di Besuki, Bangil, dan Lumajang Berdasarkan Karakter Morfologi Savitri, Lisa; Ihsan, Kharisul; Krissanjaya, Rochmad
SIMBIOSA Vol 14, No 2 (2025): SIMBIOSA
Publisher : Program Studi Pendidikan Biologi, Universitas Riau Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33373/simbiosa.v14i2.8415

Abstract

Penelitian ini bertujuan untuk mengkaji hubungan kekerabatan populasi Drosophila dari tiga lokasi di Jawa Timur, yaitu Besuki, Bangil, dan Lumajang, berdasarkan karakter morfologi. Sampel diperoleh melalui penangkapan langsung dan diamati menggunakan parameter panjang tubuh, lebar sayap, dan intensitas pigmen. Analisis deskriptif, Principal Component Analysis (PCA), dan analisis klaster digunakan untuk menentukan pola variasi dan kedekatan morfologi antar populasi. Hasil penelitian menunjukkan adanya variasi signifikan pada ketiga parameter, di mana populasi Besuki dan Bangil menunjukkan ukuran tubuh lebih besar dibandingkan Lumajang. Pigmentasi tubuh populasi Bangil lebih gelap dibandingkan dua lokasi lainnya. Analisis PCA dan klaster menunjukkan kedekatan kekerabatan Besuki–Bangil, sedangkan Lumajang membentuk kelompok tersendiri. Variasi ini diduga dipengaruhi oleh kondisi lingkungan lokal, faktor genetik, dan aspek biotik. Hasil penelitian ini menegaskan potensi penggunaan morfologi Drosophila sebagai indikator awal hubungan kekerabatan dan adaptasi lokal.
Co-Authors Elfred Rinaldo Kasimo, Elfred Rinaldo Rochmad Krissanjaya savitri, Lisa