Article Per Year (5 Year)
p-Index From 2021 - 2026
3.528
P-Index
This Author published in this journals
All Journal Biology, Medicine, & Natural Product Chemistry Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat BIO-CONS: Jurnal Biologi dan Konservasi Jurnal Kesehatan Metro Sai Wawai Jurnal Mahasiswa Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Elfred Rinaldo Kasimo, Elfred Rinaldo
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

Published : 25 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 25 Documents
Search

 1   2   3 
The Protective Effect of Kentut Leaf Extract (Paederia foetida L.) on Gastric Histopathology in Escherichia coli-Infected Sepsis Mice Model Savitri, Lisa; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.227-233

Abstract

Sepsis, a severe medical condition, signifies the systemic immune response to infection, often leading to organ dysfunction and mortality. Escherichia coli is a significant contributor to sepsis cases, particularly in gastrointestinal disorders. This study aimed to investigate the histopathological changes in the gastric tissue of mice induced by Escherichia coli infection and evaluate the protective effects of kentut leaf extract (Paederia foetida L.). Histopathological analysis revealed distinct alterations in the gastric tissue among different treatment groups. While normal mouse treatment showed no significant changes, negative control (K-) and positive control (K+) groups exhibited inflammation and hyperemia of the gastric mucosa, characterized by necrosis, degeneration, and inflammatory cell infiltration. Treatment with kentut leaf extract (P1, P2, and P3) resulted in milder inflammation compared to controls, indicating a protective effect against gastric mucosal damage induced by Escherichia coli infection. This protective mechanism is attributed to the anti-inflammatory properties of saponins, flavonoids, and essential oils present in kentut leaf extract. These findings underscore the potential therapeutic benefits of kentut leaf extract in mitigating gastric mucosal injury associated with bacterial infection.
The Correlation Between Widal Diagnostic Test, Total Leukocyte Count, and Platelet Count in Suspected Typhoid Fever Patients at RS Aura Syifa Kediri Loviana, Novi; Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.555-558

Abstract

Typhoid fever is an acute systemic infectious disease that is related to poor personal hygiene and environmental sanitation. The diagnosis of typhoid fever is established through laboratory examinations, including serological, hematological, and bacteriological tests. This research aims to determine the correlation between the Widal diagnostic test and the total leukocyte count and platelet count in suspected typhoid fever patients at RS Aura Syifa Kediri. The study design used in this research is quantitative descriptive analysis with a cross-sectional approach. The data was collected retrospectively from secondary sources, specifically medical records of patients from August 1, 2021, to March 31, 2022, at RS Aura Syifa Kediri. The total population comprised 157 patients, and a purposive sampling method was used to select 41 patients who met the inclusion and exclusion criteria. The statistical analysis using the Spearman correlation test resulted in p-values ≥ α = 0.05 for the following correlations: Widal test O with leukocytes (0.538 ≥ 0.05), Widal test H with leukocytes (0.915 ≥ 0.05), Widal test O with platelets (0.476 ≥ 0.05), and Widal test H with platelets (0.965 ≥ 0.05). These findings indicate that there is no significant correlation between the Widal test O and H with the platelet count in patients with typhoid fever. Therefore, it can be concluded that there is no significant relationship between the Widal test O and H results and the platelet count in typhoid fever patients.
An Overview of The Total Leukocyte Count in Pulmonary Tuberculosis Patients at RSUD Gambiran Kediri City Fitri, Devi Nor; Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.671-674

Abstract

The leukocyte count examination in tuberculosis infection serves to monitor the inflammation caused by Mycobacterium tuberculosis. An increasing leukocyte count can lead to complications such as pleuritis. The purpose of this study was to determine the total leukocyte count of pulmonary tuberculosis patients at RSUD Gambiran, Kediri City, based on gender and age. This research was a descriptive retrospective study. Data analysis was conducted using Microsoft Excel and presented in the form of tables and diagrams. The results of this study showed that pulmonary tuberculosis infection was more prevalent in males, with 26 individuals (62%) affected. In terms of age groups, the highest infection rate was in the 21-60 years age range, with 30 individuals (71%) affected. As for leukocyte count elevation (leukocytosis), it was more commonly observed in males with a count of 14.57 µL. In terms of age groups, leukocytosis was frequently observed in the 21-60 years age range, with a count of 48.66 µL. Out of the 42 samples, 19 individuals had a normal leukocyte count, while the remaining 23 individuals experienced leukocytosis. From the results of the study, it can be concluded that not all pulmonary tuberculosis patients experience leukocytosis; some patients maintain a normal leukocyte count.
Leaf Extract of Kentut (Paederia foetida L.) as a Preventive Measure Against Interleukin-6 Expression in the Liver of Mice in a Sepsis Model Injected with Escherichia coli Savitri, Lisa; Freitas, Maria Do Carmo Da Costa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.329-336

Abstract

Kentut leaves (Paederia foetida L.) are a medicinal plant that can be used as a preventative medicine against sepsis. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. This research aims to to determine the influence and effective dosage of Kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in a sepsis model injected with E. coli. The method used was a Completely Randomized Design (CRD). The study involved 24 white male mice divided into 6 groups. Data analysis was performed using One way ANOVA. The average values of IL-6 expression in the mouse livers for each group are as follows: KN at 7.09%±0.06; K+ at 26.36%±0.02; K- at 72.60%±0.05; PI (100mg/kgBW) at 71.04%±0.04; PII (300mg/kgBW) at 62.22%±0.02; and PIII (500mg/kgBW) at 40.92%±0.01. The research results indicate an influence of kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in the sepsis model injected with E. coli, with a significance value of 0.000 or p-value < 0.005. The effective dosage of kentut leaf extract as a preventive measure against IL-6 expression is the PIII dosage of 500mg/kg BW. The anti-inflammatory mechanism in sepsis is thought to be caused by the presence of flavonoids, alkaloids, phenolic acids, and terpenoid compounds. The most likely anti-inflammatory mechanism is believed to involve flavonoids inhibiting cyclooxygenase (COX) and lipoxygenase (LOX) enzymes involved in the synthesis of inflammatory mediators such as prostaglandins and leukotrienes, which can trigger IL-6 production.
Effect of Female Age on Crossing Over Frequency in Drosophila melanogaster Crosses N x bcl and N x ym and Their Reciprocals Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu; Antoro, Ester Lianawati; Wulansari, Ida Septika
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.267-271

Abstract

Crossing over is the occurrence of disconnection and reconnection followed by a reciprocal exchange between the two chromatids in a bivalent form. The crossing event will produce parental type and recombinant type. In the event of crossing over, various factors can influence it. These factors can be due to internal and external. Recently, various factors have been reported that influence the incidence of crossing over. These factors include age, temperature, radiation, and changes in chromosome structure. This research is a type of experimental research that uses a randomized block design. Randomized block design by crossing D. melanogaster strains ?N>< ?bcl and ?N>< ?ym and their reciprocals. From the results of this cross (F1) then cross again ?N with the recessive male (from stock) then observe the phenotype of the offspring (F2) and calculate the results of the offspring. The F2 ?N crosses were treated with age variations, namely 0, 3, 6, 9, 12, 15, and 18 days. Based on the results of these crosses, the derived strains that appeared in the F2 crosses showed the phenomenon of crossing over with the influence of the age of the female and the type of strain on crossing events. the frequency or value of crossing over (formation of recombinants) decreased with the increasing age of the female. If the age of the female affects the frequency of crossing over, then the older the female, the more likely the frequency of crossing over will decrease. However, because the data obtained were incomplete, it was not possible to know the effect of female age on the frequency of crossing over of D. melanogaster crosses ?N>< ? bcl and ?N >< ?ym and their reciprocals. The condition for crossing over is the formation of a synaptonemal complex. Age of D. melanogaster females has an effect on the frequency of crossing over in crosses N? >< bcl?, N? >< ym?, and their reciprocals. The older Drosophila melanogaster is, the lower the frequency of crossing over will occur. Based on this, it was necessary to cross D. melanogaster with strains N, bcl, and ym. A cross consists of ?N ><?bcl and ?N ><?ym and their reciprocals. By crossing ?N with a recessive male from the stock, then observing the F2 phenotype, it is hoped that crossing over will occur. So that you can better understand by doing the practice directly. In this case, the effect of crossing over is seen from the age of the female and the type of strain.
Potensi Kombinasi Pemberian Ekstrak Rosella dan Pare Menurunkan Kadar Glukosa Darah dan Meningkatkan Kolesterol-HDL pada Tikus Model Sindrom Metabolik Kasimo, Elfred Rinaldo; Savitri, Lisa; Kadir, Mujtahid Bin Abd; Alimansur, Moh; Antoro, Ester Lianawati
Jurnal Kesehatan Metro Sai Wawai Vol. 15 No. 2 (2022): Jurnal Kesehatan Metro Sai Wawai
Publisher : Poltekkes Kemenkes Tanjung Karang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jkmsaw.v15i2.3598

Abstract

Latar Belakang: Sindrom metabolik merupakan salah satu masalah kesehatan yang meningkat saat ini. Sindrome metabolik akan meningkatkan risiko diabetes melitus tipe 2 dan kardiovaskuler. Penyakit ini menjadi ancaman kesehatan sehingga dibutuhkan kombinasi obat dalam menangani berbagai gangguan disfungsi. Terapi obat kimia tidak cukup menyembuhkan atau menghentikan perkembangan penyakit. Tanaman Rosella dan pare memiliki aktifitas sebagai hipoglikemik dan antilipidemik. Tujuan: Untuk mengetahui efek kombinasi ekstrak rosella dan pare dalam menurunkan kadar glukosa darah puasa dan meningkatkan kadar HDL terhadap tikus model sindrom metabolik. Metode: Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan sampel penelitian dibagi dalam 5 kelompok perlakuan. Analisis data dengan uji paired T Test. Tahapan pelaksanaannya yaitu pengkondisian tikus sindrom metabolik. Hasil: Pada kelompok PII didapatkan penurunanan kadar glukosa darah puasa yang signifikan setelah intervensi sebesar 37,43 % (p value = 0,001). Pada kelompok PII didapatkan peningkatan kadar HDL yang signifikan setelah interveni 48,95 % (p value = 0,005) Simpulan: Potensi kombinasi pemberian ekstrak rosella dan pare mampu menurunkan kadar glukosa darah puasa dan meningkatkan kadar HDL tikus model sindrom metabolik. Simpulan: Kadar glukosa darah puasa tikus model sindrom metabolik menurun dan kadar HDL meningkat setelah diberikan intervensi kombinasi ekstrak rosella dan pare. Tahap lanjutan studi adalah untuk mengetahui uji toksisitas akut penggunaan rosella dan pare. Abstract: The potency of Combination of Rosella and Bitter Gourd Extracts to Reduce Blood Glucose Levels and Increase HDL-Cholesterol in Metabolic Syndrome Model Rats. Background: Metabolic syndrome is one of the increasing health problems today. The metabolic syndrome will increase the risk of type 2 diabetes mellitus and cardiovascular disease. This disease is a health threat that requires a combination of drugs to treat various dysfunction disorders. Chemical drug therapy is not enough to cure or stop the progression of the disease. Rosella and bitter melon plants have activities as hypoglycemic and antilipidemic. Purposes: To determine the effect of a combination of rosella and bitter melon extracts in reducing fasting blood glucose levels and increasing HDL levels in metabolic syndrome model rats. Methods: The research design used was a completely randomized design (CRD) with the research sample divided into 5 treatment groups. Data analysis with paired T-test. The stages of implementation are conditioning of metabolic syndrome rats. Result: In group PII, there was a significant decrease in fasting blood glucose levels after intervention by 37.43% (p-value = 0.001). In group PII, there was a significant increase in HDL levels after the intervention of 48.95% (p-value = 0.005) Conclusion: After receiving a mixture of rosella and bitter melon extracts, rats with the metabolic syndrome model had lower fasting blood glucose levels and higher levels of HDL. The next stage of the study was to determine the acute toxicity test of using rosella and bitter melon.
GAMBARAN NILAI HEMATOKRIT DAN JUMLAH TROMBOSIT PADA PASIEN TUBERKULOSIS PARU YANG MENDAPAT PENGOBATAN OBAT ANTI TUBERKULOSIS DI RSUD GAMBIRAN KOTA KEDIRI Muslikha, Iza Dwi; Kasimo, Elfred Rinaldo; Savitri, Lisa; Sanjaya, Rochmad Kris
Jurnal Mahasiswa Kesehatan Vol. 4 No. 2 (2023): MARET 2023
Publisher : Universitas Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/jumakes.v4i2.3687

Abstract

Tuberkulosis menjadi penyebab kematian akibat penyakit menular setelah Human Imunnodeficiency Virus (HIV). TB adalah  penyakit yang dipicu oleh basil Mycobacterium tuberculosis. Tujuan penelitian ini adalah untuk mengetahui indikator anemia dan kelainan trombosit terhadap pesien TB paru yang mendapat pengobatan OAT di RSUD Gambiran Kota Kediri. Metode yang digunakan dalam penelitian ini ialah deskriptif dengan desainnya cross sectional. Peneilitian ini dilaksanakan pada bulan Mei-Agustus 2022. Populasi dari penelitian ini ialah seluruh pasien TB paru yang mendapat pengobatan OAT yang ada di catatan rekam medik pada bulan Januari-Desember 2021 sejumlah 43 sampel yang sesuai dengan kriteria inklusi dan ekslusi. Teknik sampling yang dipakai ialah purposive sampling. Hasil penelitian ini adalah Indikator dari anemia adalah penurunan nilai hematokrit yang disebabkan oleh efek dari terapi OAT. Sedangkan indikator dari kelainan trombosit adalah yaitu terjadinya penurunan (trompositopenia) dan peningkatan jumlah trombosit (trombositosis) yang disebabkan oleh efek dari terapi OAT. Sebagian besar pasien mengalami penurunan nilai hematokrit sebanyak 33 orang (77 %). Sebanyak 25 orang (58 %) memiliki nilai trombosit yang normal, 3 orang (7 %) mempunyai nilai trombosit yang rendah dan 15 orang (35 %) memiliki nilai trombosit yang tinggi.
GAMBARAN MCV DAN MCH PADA PENDERITA TUBERKULOSIS PARU DI RSUD GAMBIRAN KOTA KEDIRI Faradita, Firda Fredenna; Kasimo, Elfred Rinaldo; Sanjaya, Rochmad Kris
Jurnal Mahasiswa Kesehatan Vol. 4 No. 2 (2023): MARET 2023
Publisher : Universitas Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/jumakes.v4i2.3693

Abstract

Tuberkulosis paru merupakan masalah kesehatan yang dapat menyebabkan komplikasi. Salah satu komplikasi yaitu anemia, maka dari itu diperlukan pemeriksaan indeks eritrosit Mean Corpuscular Volume (MCV) dan Mean Corpuscular Hemoglobin (MCH) untuk mendiagnosis jenis anemia. Penelitian ini bertujuan mengetahui gambaran MCV dan MCH pada pasien tuberkulosis paru di RSUD Gambiran Kota Kediri. Metode yang digunakan adalah metode deskriptif observasional dengan desain cross sectional. Populasi berjumlah 76 pasien tuberkulosis paru di RSUD Gambiran Kota Kediri periode Januari-Desember 2021. Sampel berjumlah 43 orang sesuai kriteria inklusi dan eksklusi (purposive sampling) menggunakan data rekam medis. Hasilnya terdapat 33 orang memiliki nilai MCV rendah (mikrositik) dan 27 orang memiliki MCH rendah (hipokromik). Kadar MCV dan MCH rendah dikarenakan kekurangan asupan zat besi. Zat besi dapat berkurang disebabkan oleh infeksi bakteri Mycobaterium tuberculosis. Bakteri tersebut menyebabkan peradangan yang kemudian menghambat produksi hemoglobin dalam eritrosit. Akibatnya eritrosit tidak berfungsi maksimal untuk mengedarkan oksigen ke seluruh tubuh dan kualitas tubuh menjadi menurun. Pasien tuberkulosis paru di RSUD Gambiran Kota Kediri periode Januari-Desember 2021 sebagian besar mengalami anemia mikrositik hipokromik.
Histopathological Description of Mouse Liver in a Sepsis Model Infected with Escherichia coli Treated with Paederia foetida L. Leaf Extract for Sepsis Prevention Savitri, Lisa; Retnowati, Ana; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.485-492

Abstract

The leaf of Paederia foetida L. is one type of medicinal plant that can be used as a preventive medicine for sepsis. This plant contains secondary metabolites such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. The objective of this study was to determine the histopathological description of the liver in a mouse sepsis model infected with E. coli, with the administration of Paederia foetida L. leaf extract for sepsis prevention, and to ascertain the influence and effective dosage of the leaf extract as a preventive measure against liver histopathology in the sepsis model induced by E. coli. The method employed was a Completely Randomized Design (CRD). The study used 24 male white mice divided into 6 (six) groups. Data analysis was conducted using One Way ANOVA. The results of the study revealed the histopathological profile of liver cell degeneration in group PI (100mg/kg BW) at 20.79%±0.03, group PII (200mg/kg BW) at 21.63%±0.02, and group PIII (500mg/kg BW) at 9.08%±0.02. Necrosis rates were observed in group PI (100mg/kgBW) at 22.62%±0.04, group PII (200mg/kg BW) at 17.63%±0.02, and group PIII (500mg/kg BW) at 6.05%±0.02. The presence of polymorphonuclear leukocytes (PMN) was detected in group PI (100mg/kgBW) at 39.56%±0.03, group PII (200mg/kgBW) at 28.05%±0.02, and group PIII (500mg/kg BW) at 18.45%±0.03. The test results showed a significant effect of P. foetida L. leaf extract as a preventive measure against liver histopathology in the mouse sepsis model infected with E. coli, with significant values for necrosis (p=0.000), cell degeneration (p=0.000), and PMN (p=0.000). The most effective dosage of P. foetida L. leaf extract as a preventive measure against liver histopathology in the mouse sepsis model infected with E. coli was the dosage used in group PIII (500mg/kgBW).
THE EFFECT OF Paederia foetida L. EXTRACT ON LIVER WEIGHT OF MICE SEPSIS MODEL INFECTED WITH Escherichia coli Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8965

Abstract

Sepsis is a critical medical condition characterized by a systemic immune response to in-fection, which can lead to severe organ dysfunction and mortality. Bacterial sepsis, par-ticularly caused by Escherichia coli, poses a significant risk due to its potential to harm tissues and organs, including the liver. The liver plays a central role in metabolic process-es and host defense during sepsis, making it a key organ of interest. This study aimed to investigate the effect of Paederia foetida leaf extract on liver weight in a mice sepsis mod-el. Mice were divided into six groups: normal control (N), negative control (K-) receiving distilled water, positive control (K+) receiving ciprofloxacin, treatment 1 (P1) receiving P. foetida extract at 100 mg/kg BW, treatment 2 (P2) receiving 300 mg/kg BW, and treat-ment 3 (P3) receiving 500 mg/kg BW. After 14 days of treatment, significant differences in liver weight were observed among the groups, with the highest mean and standard de-viation recorded in the P1 group (1.3750 ± 0.3932). Liver abnormalities, including swell-ing, lobular thickening, and weight increase, were identified, indicating the liver’s adap-tive response to toxic substances during sepsis. Interestingly, the normal control group exhibited higher liver weights compared to the treatment groups, possibly due to fatty substance accumulation within the liver tissues. These findings suggest that P. foetida ex-tract may influence liver weight changes in sepsis, potentially modulating metabolic and detoxification processes. Further studies focusing on histopathological and biochemical mechanisms are needed to clarify the therapeutic potential of P. foetida in managing liver dysfunction associated with sepsis.
Co-Authors Alimansur, Moh Amanda, Cornelia Anjarwati, Aprilia Antoro, Ester Lianawati Apriana, Dita Datin An Nisa Sukmawati Dhony Hermanto Faradita, Firda Fredenna Fendy Prasetyawan Fitri, Devi Nor Freitas, Maria Do Carmo Da Costa Hilmi, Mochamad Hanif Ihsan, Kharisul Juwita, Syntia Tanu Kadir, Mujtahid Bin Abd Kholis, Akbar Nur khusnul khotimah Loviana, Novi Mebung, Konradus Klala Mosse1, Yosep Muslikha, Iza Dwi Muttaqin, Saiful Nurul Ismillayli Rasyadan Taufiq Probojati Retnowati, Ana Rochmad Krissanjaya Sanjaya, Rochmad Kris savitri, Lisa Wulansari, Ida Septika Yanti, Novirma Yuneka Saristiana