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All Journal JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian
Qurata Aini, Luqman
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Agriculture, Biological Sciences & Forestry Environmental Science

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Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora Muhidin, Ahmad; Hapsari, Femita; Qurata Aini, Luqman; Akhid Syib’li, Muhammad; Rizkyta Kusuma, Restu
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.58-71.2025

Abstract

Soft rot is an important potato disease caused by the bacterial pathogen Erwinia carotovora. Although chemical bactericides are commonly used for control, biological control using antagonistic bacteria offers a promising and environmentally friendly alternative. Bacteria capable of surviving in extreme environments have been reported to show high efficiency and the ability to withstand various environmental conditions. This study aimed to isolate, evaluate the potential and activity, and identify the molecular basis of novel bacteria from Sidoarjo hot mud against E. carotovora, the causal agent of potato soft rot. The methods used included sample collection, bacterial isolation and screening, inhibition assays against E. carotovora, soft rot suppression assays on potato tubers, molecular identification using 16S rRNA gene sequencing, and antibiosis mechanism testing. Based on the research, 16 bacterial isolates were obtained, of which four isolates (BLS3, BLS6, BLS7, and BLS10) demonstrated antagonistic activity, as evidenced by the formation of clear inhibition zones on nutrient agar medium. Suppression assays further confirmed their ability to significantly reduce soft rot symptoms in potato tubers. Among these, BLS10 isolate exhibited the highest inhibition and suppression activity, with an inhibition zone of 2.03 cm and a soft rot mass of 0.52 grams. Molecular identification based on 16S rRNA gene sequencing showed that isolates of BLS3, BLS6, BLS7, and BLS10 were identified respectively as Bacillus velezensis, Bacillus methylotrophicus, Bacillus amyloliquefaciens, and Ochrobactrum intermedium. All isolates demonstrated a bacteriostatic antibiosis mechanism, indicated by turbidity in peptone water, suggesting inhibition through secondary metabolite production.
KARAKTERISASI BAKTERI PATOGEN PENYEBAB LAYU PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.) Fuji Arriani, Intan; Latief Abadi, Abdul; Qurata Aini, Luqman
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 14 No 1 (2020): Mei 2020
Publisher : Universitas Islam Balitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35457/viabel.v14i1.1004

Abstract

Shallot (Allium ascalonicum L.) is one of the vegetable commodities in the form of tubers that have a high economic value. The development of shallot cultivation in Indonesia often experiences obstacles, one of which is an obstacle in the process of shallot cultivation, namely the attack of Plant Disturbing Organisms (OPT). Information about diseases caused by pathogenic bacteria is still very limited. This study aims to determine the symptoms and identification of pathogenic bacteria that cause wilt in shallots. Bacterial isolation was carried out using Nutrient Agar (NA) media and 36 bacterial isolates were collected from shallots. Bacterial isolates were then tested for pathogenicity to determine the ability of bacteria to cause wilt disease in shallots. The results of isolation obtained 10 bacterial isolates that can show symptoms on red onions namely wilted leaves, yellow and soft rotten tubers. Four isolates including positive can show hypersensitivity symptoms, namely M11, N20, N17 and N14. Based on the identification of bacteria in physiology showed 2 groups of different isolates. Biochemical test results of Isolate M11, N20 and show species suspected of B. cepacia. N3 and N14 isolates are suspected to be E. carotavora subsp. carotavora. The bacterial isolates N7, N17, P5 and P7 were suspected to be E. carotavora subsp. betavasculorum. The isolate of N4 bacteria is suspected to be E. cacticida.
Co-Authors Abdul Latief Abadi Akhid Syib’li, Muhammad Fuji Arriani, Intan Hapsari, Femita Muhidin, Ahmad Rizkyta Kusuma, Restu