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All Journal JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION)
Muhidin, Ahmad
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Agriculture, Biological Sciences & Forestry Environmental Science

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Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora Muhidin, Ahmad; Hapsari, Femita; Qurata Aini, Luqman; Akhid Syib’li, Muhammad; Rizkyta Kusuma, Restu
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.58-71.2025

Abstract

Soft rot is an important potato disease caused by the bacterial pathogen Erwinia carotovora. Although chemical bactericides are commonly used for control, biological control using antagonistic bacteria offers a promising and environmentally friendly alternative. Bacteria capable of surviving in extreme environments have been reported to show high efficiency and the ability to withstand various environmental conditions. This study aimed to isolate, evaluate the potential and activity, and identify the molecular basis of novel bacteria from Sidoarjo hot mud against E. carotovora, the causal agent of potato soft rot. The methods used included sample collection, bacterial isolation and screening, inhibition assays against E. carotovora, soft rot suppression assays on potato tubers, molecular identification using 16S rRNA gene sequencing, and antibiosis mechanism testing. Based on the research, 16 bacterial isolates were obtained, of which four isolates (BLS3, BLS6, BLS7, and BLS10) demonstrated antagonistic activity, as evidenced by the formation of clear inhibition zones on nutrient agar medium. Suppression assays further confirmed their ability to significantly reduce soft rot symptoms in potato tubers. Among these, BLS10 isolate exhibited the highest inhibition and suppression activity, with an inhibition zone of 2.03 cm and a soft rot mass of 0.52 grams. Molecular identification based on 16S rRNA gene sequencing showed that isolates of BLS3, BLS6, BLS7, and BLS10 were identified respectively as Bacillus velezensis, Bacillus methylotrophicus, Bacillus amyloliquefaciens, and Ochrobactrum intermedium. All isolates demonstrated a bacteriostatic antibiosis mechanism, indicated by turbidity in peptone water, suggesting inhibition through secondary metabolite production.
Potential of PGPR from Rhizospheric Grass in Bromo Tengger Semeru National Park, Indonesia, against Xanthomonas oryzae pv. oryzae Hapsari, Femita; Muhidin, Ahmad; Aini, Luqman Qurata; Rachmawati, Rina
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.14-25.2025

Abstract

Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most destructive diseases affecting rice, resulting in significant yield losses and reduced grain quality. The overuse of chemical bactericides has raised environmental concerns and contributed to the emergence of resistant pathogen strains, emphasizing the need for sustainable alternatives. Plant Growth-Promoting Rhizobacteria (PGPR) offer a promising alternative due to their dual role in enhancing plant growth and suppressing phytopathogens. This study aimed to isolate and characterize PGPR from the rhizosphere of Imperata cylindrica, a grass species thriving in the extreme sand sea environment of Bromo Tengger Semeru National Park (BTSNP), Indonesia. The sampling site represents a unique volcanic soil ecosystem characterized by sandy texture, high surface temperatures, and nutrient-poor conditions, with no vegetation other than I. cylindrica. These extreme environmental conditions suggest that the associated microbial communities may be highly adapted to abiotic stress and possess valuable traits for plant growth promotion and pathogen suppression. A total of 46 rhizobacterial isolates were obtained, of which five (Isolates 5, 6, 8, 10, and 12) demonstrated nitrogen-fixing ability, phosphate-solubilizing activity, and strong antagonism against Xoo. Morphological, physiological, and biochemical analyses identified Isolates 5, 8, and 12 as Pantoea spp., while Isolates 6 and 10 were classified as Erwinia spp. In vitro inhibition assays revealed that Isolate 6 exhibited the highest antagonistic activity, comparable to streptomycin, and was categorized as "Very Strong." These findings highlight Isolate 6 as a promising candidate for development as a biological control agent for sustainable rice disease management.
Co-Authors Akhid Syib’li, Muhammad Hapsari, Femita Luqman Qurata Aini Qurata Aini, Luqman Rina Rachmawati Rizkyta Kusuma, Restu