Tobacco (Nicotiana tabacum L.) is an important commodity due to its high economic value and utilisation. However, tobacco productivity is still suboptimal due to soil salinity. One effort that can be made to overcome salinity stress is to develop more tolerant tobacco varieties. To increase plant tolerance to salinity stress, seed priming and the application of silicon-based fertilisers to the growing medium can be carried out. Seed priming treatments, which control seed hydration, can increase seed germination. Silicon (Si) is a beneficial element that plays a role in increasing plant tolerance to stress. This study aims to examine the effect of seed priming and calcium silicate application on 'Jember Sili' tobacco in saline soil by observing germination, growth, and physiological responses. This Completely Randomized Design (CRD) study consisted of two factors: seed priming with calcium silicate (0 mM, 1 mM, 2 mM) and calcium silicate (CaSiO3) addition to the growing medium (0 mM, 1 mM, 2 mM). Data were analysed using SPSS for ANOVA, followed by DMRT at a 95% significance level. Tobacco (Nicotiana tabacum L. 'Jember Sili') responded positively to varying concentrations of calcium silicate priming, particularly at 1 mM, as indicated by a significant increase in germination percentage, germination rate, vigour index, Timson Germination Index (TGI), and Mean Germination Time (MGT). The 1 mM concentration was also optimal for increasing plant height, leaf number, dry weight of shoots and roots, and root length. Tobacco priming and calcium silicate application in low-saline soil also increased chlorophyll, carotenoid, and SOD levels. But it reduces H2O2 levels and MDA levels.