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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Journal of halal product and research (JHPR)
Sholahuddin, Muhammad Athoillah
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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PENGARUH PERBEDAAN KONSENTRASI ENZIM BROMELIN PADA HIDROLISAT PROTEIN DARI LIMBAH JEROAN IKAN NILA (Oreochromis sp.) TERHADAP AKTIVITAS ANTIOKSIDAN Sholahuddin, Muhammad Athoillah
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 19, No 4 (2023): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.19.4.203-208

Abstract

Ikan nila merupakan komoditas andalan budidaya yang cukup banyak dikonsumsi oleh masyarakat luas, khususnya di Indonesia. Jeroan ikan nila segar memiliki komposisi kadar protein sebanyak 55,55% sehingga untuk meningkatkan nilai jualnya seringkali digunakan sebagai hidrolisat protein. Hidrolisis protein menggunakan enzim merupakan cara yang efektif, karena dapat membuat hidrolisat protein terhindar dari kerusakan asam amino tertentu. Enzim proteolitik memiliki banyak macam dan yang dapat digunakan sebagai penghidrolisis, dalam menghasilkan hidrolisat protein adalah enzim bromelin. Tujuan penelitian ini untuk mengetahui pengaruh jenis enzim proteolitik dan waktu hidrolisis terhadap aktivitas antioksidan dari hidrolisat protein dari limbah jeroan ikan nila Oreochromis sp. Penelitian ini menggunakan RAL 2 faktor dan masing-masing diulang sebanyak 3 kali. Faktor pertama yaitu konsentrasi enzim bromelin yang digunakan yakni konsentrasi kontrol, 1%, 2% dan 3%. Faktor yang kedua yaitu waktu hidrolisis antara lain 4 jam, 6 jam dan 8 jam. Data yang diperoleh dianalisis dan dilanjutkan dengan uji aktivitas antioksidan, uji derajat hidrolisis dan uji kadar protein. Hasil Penelitian menunjukkan bahwa konsentrasi enzim bromelin terbaik sebagai antioksidan adalah 3% dan waktu hidrolisis 6 jam dengan nilai IC50  89.91 µg/mL. Sementara Derajat Hidrolisis terbaik terdapat pada konsentrasi enzim 3%  dan waktu hidrolisis 6 jam dengan DH hingga 82,25%. Kadar Protein tertinggi pada perlakuan P2 6 jam (konsentrasi enzim bromelin 2%) sebesar 59,31%.
The Development of antioxidant products from Tilapia offal protein hydrolysate with different enzyme concentrations and a review in fulfillment of the halal product assurance criteria Sholahuddin, Muhammad Athoillah; Prayoga, Adistiar
Journal of Halal Product and Research (JHPR) Vol. 6 No. 2 (2023): The Development of Global Halal: Issues and Challenges
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jhpr.vol.6-issue.2.138-146

Abstract

Tilapia fish meat is a raw material that is widely consumed by the community due to its high nutritional value. However, the amount of meat consumption is not accompanied by the management of tilapia by-products. By-products from the disposal of tilapia processing can cause pollution and disrupt certain ecosystems, so it is necessary to manage the waste by-products of tilapia products. One way to process tilapia offal waste is by utilizing it as a protein hydrolysate product. Protein hydrolysate is a derivative product of protein in the form of bioactive peptides and amino acids that have potential as antioxidants. Products from antioxidants have many benefits such as anti-aging, as food supplements, as drugs to ward off cancer and so on. However, antioxidant products sold commercially are relatively expensive when compared to tilapia offal waste protein hydrolysate products. Therefore, this protein hydrolysate product is a suitable means to be an alternative as a substitute for commercial antioxidants. However, from another perspective, waste that is regarded as inappropriate production residue is really used as a raw material for consumable. Meanwhile, the Indonesia government has legalized law number 33 of 2014 oh Halal Product Assurance, which requires that all products entering, circulating, and traded in Indonesia be halal certified. So, the purpose of this study, (1) investigate the (1) investigate the influence of proteolytic enzymes at different doses on antioxidant activity in tilapia offal waste protein hydrolysate. (2) Review product development to ensure that it meets halal product assurance requirements in Indonesia and becomes a raw material or additional material that can be halal certified
Co-Authors Prayoga, Adistiar