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All Journal HAYATI Journal of Biosciences Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Memili, Erdogan
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Social Sciences

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Application of Proteomics to Identify Fertility Markers in Angus Bull Sperm Kutchy, Naseer A.; Dogan, Sule; Wang, Xiaojun; Topper, Einko; Kaya, Abdullah; Memili, Erdogan
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.774 KB) | DOI: 10.4308/hjb.27.2.116

Abstract

The goal of the study was to ascertain sperm proteins as fertility markers by identifying sperm proteins in Angus bull sperm using proteomics and validate the markers through comparative sperm biology between Angus and Holstein bulls for which there is reliable fertility data available. We aimed to determine proteins differentially expressed in sperm from Angus bulls with different fertility phenotypes. Two-dimensional differential gel electrophoresis with mass-spectrometry, functional gene clusters, canonical pathways and protein networks, using integrated discovery bioinformatics software and ingenuity pathway analysis were used to identify and analyze sperm proteome. We identified 80 proteins that were differentially expressed in sperm of our experimental population. Using computational biology approaches we demonstrated involvement of structural proteins such as outer dense fiber of sperm tails 2 and enzymes including kinases, and phosphatases having functions in essential pathways in glycolysis/gluconeogenesis and free scavenging. The results are significant because analyzed proteins in Angus sperm are determinants of fertility, gene-environment interactions, as well as potential biomarkers for animal breeding.
Pengembangan Penanda Fertilitas Sebagai Alat Bantu “Akurat” Dalam Upaya Optimalisasi Sapi Pejantan Unggul Dalam Program Inseminasi Buatan Puwantara, Bambang; Arifiantini, Iis; Karja , Ni Wayan Kurniani; Pardede , Berlin Pandapotan; Indriastuti, Rhesti; Satrio , Faisal Amri; Memili, Erdogan
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 1 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0401.220-224

Abstract

Peningkatan populasi dan mutu ternak, khususnya sapi dan kerbau d ilakukan melalui program inseminasi buatan (IB). Angka kebuntingan hasil IB di Indonesia, relatif masih rendah, salah satunya diduga disebabkan oleh fertilitas pejantan. Program IB di Indonesia menggunakan semen beku yang diproduksi oleh Balai Inseminasi Buatan (BIB). Standar evaluasi semen bagi pejantan yang digunakan sesuai SNI masih mengacu pada konsentrasi, motilitas pra dan pasca thawing serta skor individu. Perkembangan terbaru menunjukkan bahwa parameter uji tersebut tidak lagi memadai. Protein dan gen pada spermatozoa dan seminal plasma pejantan dapat menjadi kandidat alat bantu “akurat” untuk menentukan fertilitas pejantan sesuai keberhasilan IB di lapangan. Policy brief ini diharapkan dapat menjadi bahan pertimbangan bagi Pemerintah dan BIB dalam p engembangan dan pengaplikasian penanda fertilitas berbasis molekuler sebagai alat bantu “akurat” dalam proses seleksi dan kebijakan pengafkiran pejantan sapi yang akan digunakan untuk IB. Dengan demikian, efisiensi penggunaan pejantan unggul di berbagai Ba lai Inseminasi Buatan, baik Nasional maupun Daerah di Indonesia dapat dioptimalkan.
Co-Authors Dogan, Sule Faisal Amri Satrio Indriastuti, Rhesti Kaya, Abdullah Kutchy, Naseer A. Ni Wayan Kurniani Karja Pardede , Berlin Pandapotan Puwantara, Bambang R. Iis Arifiantini Topper, Einko Wang, Xiaojun