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All Journal HAYATI Journal of Biosciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Memili, Erdogan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Social Sciences Veterinary

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Application of Proteomics to Identify Fertility Markers in Angus Bull Sperm Kutchy, Naseer A.; Dogan, Sule; Wang, Xiaojun; Topper, Einko; Kaya, Abdullah; Memili, Erdogan
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.774 KB) | DOI: 10.4308/hjb.27.2.116

Abstract

The goal of the study was to ascertain sperm proteins as fertility markers by identifying sperm proteins in Angus bull sperm using proteomics and validate the markers through comparative sperm biology between Angus and Holstein bulls for which there is reliable fertility data available. We aimed to determine proteins differentially expressed in sperm from Angus bulls with different fertility phenotypes. Two-dimensional differential gel electrophoresis with mass-spectrometry, functional gene clusters, canonical pathways and protein networks, using integrated discovery bioinformatics software and ingenuity pathway analysis were used to identify and analyze sperm proteome. We identified 80 proteins that were differentially expressed in sperm of our experimental population. Using computational biology approaches we demonstrated involvement of structural proteins such as outer dense fiber of sperm tails 2 and enzymes including kinases, and phosphatases having functions in essential pathways in glycolysis/gluconeogenesis and free scavenging. The results are significant because analyzed proteins in Angus sperm are determinants of fertility, gene-environment interactions, as well as potential biomarkers for animal breeding.
Pengembangan Penanda Fertilitas Sebagai Alat Bantu “Akurat” Dalam Upaya Optimalisasi Sapi Pejantan Unggul Dalam Program Inseminasi Buatan Puwantara, Bambang; Arifiantini, Iis; Karja , Ni Wayan Kurniani; Pardede , Berlin Pandapotan; Indriastuti, Rhesti; Satrio , Faisal Amri; Memili, Erdogan
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 1 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0401.220-224

Abstract

Peningkatan populasi dan mutu ternak, khususnya sapi dan kerbau d ilakukan melalui program inseminasi buatan (IB). Angka kebuntingan hasil IB di Indonesia, relatif masih rendah, salah satunya diduga disebabkan oleh fertilitas pejantan. Program IB di Indonesia menggunakan semen beku yang diproduksi oleh Balai Inseminasi Buatan (BIB). Standar evaluasi semen bagi pejantan yang digunakan sesuai SNI masih mengacu pada konsentrasi, motilitas pra dan pasca thawing serta skor individu. Perkembangan terbaru menunjukkan bahwa parameter uji tersebut tidak lagi memadai. Protein dan gen pada spermatozoa dan seminal plasma pejantan dapat menjadi kandidat alat bantu “akurat” untuk menentukan fertilitas pejantan sesuai keberhasilan IB di lapangan. Policy brief ini diharapkan dapat menjadi bahan pertimbangan bagi Pemerintah dan BIB dalam p engembangan dan pengaplikasian penanda fertilitas berbasis molekuler sebagai alat bantu “akurat” dalam proses seleksi dan kebijakan pengafkiran pejantan sapi yang akan digunakan untuk IB. Dengan demikian, efisiensi penggunaan pejantan unggul di berbagai Ba lai Inseminasi Buatan, baik Nasional maupun Daerah di Indonesia dapat dioptimalkan.
Review: Solutions for Grand Challenges in Goat and Sheep Production Industry Aldridge, Mildred Elizabeth; Fearon, Jasmyn Elaine; Haynes, Bethany Peyton; Miller, Hannah Marie; Sanford, Katie Yvonne; Scott, Rachel Rebecca; Anglin, William Wess; Blalock, Lauren Susanne; Burkes, Briana Lashun; Cohn-White, Olivia Lucienne; Franks, Brittany Renee; Giles, Haleigh Margaret; Greene, Ashley Lorraine; Hanby, Riley Dawn; Holliman, Anna Grace; Kirby, John Mark; Klein, Austin William; Lehmann, Courtney Ann; Llyod, Grace Jennette; Lore, Cody Triston; McMurray, Tyler B.; Moody, Zachary Vinz; Palmer, Brianna Nicole; Pansano, Lindsey Virginia; Pickle, Ryan Matthew; Schaeffer, Laura Marie; Seidl, Jeneva Ruth; Smith, James Dean; Stepp, Hannah Faith; Satrio, Faisal Amri; Kutchy, Naseer Ahmad; Dechert, Ed; Rutherford, Cobie; Brown, Kipp; Purwantara, Bambang; Memili, Erdogan
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.511 KB) | DOI: 10.11598/btb.2019.26.1.944

Abstract

Goats and sheep are valuable as they are a source of meat, milk, fleece, and other products. These livestock are also important both for agriculture and biomedical research. However, the efficient, sustainable, and profitable production of these small ruminants faces major obstacles. Hence, this review analyzes these major challenges specifically, their negative impacts on the industry, and suggests some science-based solutions to overcome them. Those challenged areas are education and training, research, translational research/biotechnology, goat and sheep health, and maintenance of an economically sustainable agribusiness. The suggested solutions include the effective teaching of goat and sheep science to the next generation and public empowerment, support for innovative and translational research, disease prevention and treatment, support for technology transfer, and development of sound agribusiness practices. This review is helpful particularly for scientists, students, and the goat and sheep producers. In general, these information on the current state of goat and sheep agriculture will also help the public to better understand and appreciate the challenges met and opportunities provided in small ruminant production enterprises.
Co-Authors Aldridge, Mildred Elizabeth Anglin, William Wess Bambang Purwantara Blalock, Lauren Susanne Brown, Kipp Burkes, Briana Lashun Cohn-White, Olivia Lucienne Dechert, Ed Dogan, Sule Faisal Amri Satrio Fearon, Jasmyn Elaine Franks, Brittany Renee Giles, Haleigh Margaret Greene, Ashley Lorraine Hanby, Riley Dawn Haynes, Bethany Peyton Holliman, Anna Grace Indriastuti, Rhesti Kaya, Abdullah Kirby, John Mark Klein, Austin William Kutchy, Naseer A. Kutchy, Naseer Ahmad Lehmann, Courtney Ann Llyod, Grace Jennette Lore, Cody Triston McMurray, Tyler B. Miller, Hannah Marie Moody, Zachary Vinz Ni Wayan Kurniani Karja Palmer, Brianna Nicole Pansano, Lindsey Virginia Pardede , Berlin Pandapotan Pickle, Ryan Matthew Puwantara, Bambang R. Iis Arifiantini Rutherford, Cobie Sanford, Katie Yvonne Schaeffer, Laura Marie Scott, Rachel Rebecca Seidl, Jeneva Ruth Smith, James Dean Stepp, Hannah Faith Topper, Einko Wang, Xiaojun