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All Journal Hemera Zoa Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology ARSHI Veterinary Letters Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Buletin Pengabdian Policy Brief Pertanian, Kelautan, dan Biosains Tropika

Faisal Amri Satrio

Veterinary Medicine Study Program, Faculty Of Medicine, Padjajaran University, Bandung, Indonesia

Author-ID : 8537898

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

Published : 12 Documents Claim Missing Document

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PCS-4 In Vivo Embryo Production at Cipelang Livestock Embryo Centre Faisal Amri Satrio; Ni Wayan Kurniani Karja; M Imron; Yuni Siswani; Oloan Parlindungan; Bambang Purwantara
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.888 KB)

Livestock Embryo Center/Balai Embrio Ternak (BET) Cipelang is one of the government institution under the supervision of Directorate General of Livestock and Animal Health Services, Ministry of Agriculture of the Republic of Indonesia. BET Cipelang has the main task of carrying out the production, development, and distribution of livestock embryo for all regions of Indonesia. BET Cipelang produced embryos using two methods, there are in vivo and in vitro. The produced embryos will be transferred to the recipient cow using embryo transfer technique. Embryo transfer biotechnology can be used to enhance genetic improvement and to increase marketing opportunities with purebred cattle. Because of their relatively low reproductive rate and long generation interval, embryo transfer is especially useful with cattle. The success of embryo transfer depends on factors associated with the embryo, the recipient or an interaction among the factors of the embryo and recipient [1]. Embryo quality is one of the main factors for successful embryo production. In vivo embryo production has better quality than in vitro such as cell number, morphology, growth and development ability and after freezing. The frequency of occurrence of chromosomal abnormalities in in vivo-derived embryos are lower than in vitro and the consequence is that only 30-40% of oocytes resulting from in vitro maturation develop into blastocysts after in vitro fertilization and the in vitro yield rate of embryos are lower than from in vivo [2]. Recently, there are no studies about the performance of in vivo embryo production at BET Cipelang. The aim of this study is to explore the quality of in vivo embryos produced by BET Cipelang.

Gambaran Histopatologi Kauda Epididimis Domba yang Disimpan pada Suhu 4ºC dalam Dulbecco’s Modified Eagle Medium Faisal Amri Satrio; Sri Estuningsih; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk menganalisis kerusakan jaringan kauda epididimis domba selama empat hari penyimpanan dengan atau tanpa DMEM pada suhu 4ºC. Sebanyak 15 pasang kauda epididimis dikoleksi dari tempat pemotongan hewan dan disimpan dengan cara salah satu dari setiap pasang kauda epididimis dimasukan ke dalam DMEM dan bagian lainnya disimpan tanpa menggunakan DMEM. Preparat histopatologi jaringan kauda epidimis dilakukan setelah penyimpanan pada suhu 4ºC (H-0), lalu dilanjutkan setelah penyimpanan pada suhu 4ºC selama 24 jam (H-1), 48 jam (H-2), 72 jam (H-3), dan 96 jam (H-4). Hasil penelitian menunjukkan bahwa kapsula kauda epididimis secara nyata mengalami penebalan pada H-2 untuk penyimpanan tanpa DMEM dan H-4 untuk penyimpanan menggunakan DMEM (P<0,05). Kerusakan epitel kauda epididimis mengalami peningkatan mulai H-1, namun jumlah kerusakan di H-4 pada penyimpanan menggunakan DMEM lebih sedikit dibandingkan tanpa menggunakan DMEM (P<0,05). Penelitian ini menyimpulkan bahwa penyimpanan kauda epididimis pada suhu 4ºC menggunakan DMEM dapat mengurangi tingkat kerusakan epitel kauda epididimis hingga jam ke-96 dan memperlambat kerusakan kapsula kauda epididimis hingga jam ke-48.

Metode nephrosplenic entrapment reposition untuk penanganan kasus large colon displacement pada kuda Hadi Putra Rihansyah; Agus Wijaya; Yohan Naim Nurul Fatonah; Wahyu Sri Wulandari; Faisal Amri Satrio; Muhammad Elmanaviean; Intan Maria Paramita; Citra Ayu Lestari
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Large colon displacement merupakan perpindahan posisi kolon menjadi ke arah kiri disebut left dorsal displacement of colon (LDDC), atau ke arah kanan disebut right dorsal displacement of colon (RDDC). Perpindahan ini menyebabkan kolik pada kuda. Palpasi per rektal pada kuda G. Galunggung menunjukkan adanya perubahan konsistensi dan perpindahan lokasi kolon sehingga dugaan utama adalah terjadinya large colon displacement. Palpasi per rektal daerah nephrosplenic menunjukkan limpa pindah ke arah caudal di dinding kiri abdomen. Hal ini menunjukkan bahwa kuda G. Galunggung mengalami LDDC. Reposisi mekanis metode nephrosplenic entrapment reposition menggunakan katrol dilakukan untuk mengembalikan posisi kolon yang terperangkap di antara ginjal dan limpa setelah kuda G. Galunggung dibius terlebih dahulu. Konfirmasi secara palpasi per rektal menunjukan bahwa metode tersebut berhasil mengembalikan posisi kolon pada kasus kuda G. Galunggung. Setelah 3 hari perlakuan, kuda makan dan minum secara normal, konsitensi feses normal, serta tidak terjadi kolik maupun ambruk.

QUALITY OF CHILLED CANINE SEMEN IN TRIS-EGG YOLK EXTENDER SUPPLEMENTED WITH SERICIN Kim Chwin Khye; Tuty Laswardi Yusuf; Faisal Amri Satrio; Ni Wayan Kurniani Karja
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

ABSTRACTThe objective of this research was to evaluate the quality of chilled canine semen in Tris-egg yolk (TEY) extenders containing different concentrations of sericin. Semen were collected from four dogs by massage method. Canine semen was collected using sterile urine pots and evaluated. Sperm-rich fractions were pooled and divided into four equal aliquots, which were then diluted with TEY extenders supplemented with different concentrations of sericin (0%, 0.1%, 0.25%, 0.5%). The diluted semen aliquots were preserved at 4 ℃ in sterile centrifuge tubes and were then evaluated for spermatozoa motility, viability, plasma membrane integrity and acrosome integrity every 12 hours up to 72 h. The TEY extenders supplemented with 0.25% and 0.5% sericin resulted in higher spermatozoa motility and viability at 72 h compared to other TEY extenders (P0.05). The integrity of plasma membrane and acrosome of spermatozoa showed no significant differences among the groups extenders at 72 h. In conclusion, sericin in concentration of 0.25% and 0.5% were able to prevent the motility and viability of canine spermatozoa after storage for 72 h.

PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Faisal Amri Satrio; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin; Asep Kurnia; Bambang Purwantara
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

This study aimed to determine the effect of age on bulls’ productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, ≥ 10 yo with high semen rejection (≥10 HR), and ≥ 10 yo with low semen rejection (≥10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of ≥10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in ≥ 10 yo groups. The ≥10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of ≥10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in ≥10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.

Pengembangan Penanda Fertilitas Sebagai Alat Bantu “Akurat” Dalam Upaya Optimalisasi Sapi Pejantan Unggul Dalam Program Inseminasi Buatan Puwantara, Bambang; Arifiantini, Iis; Karja , Ni Wayan Kurniani; Pardede , Berlin Pandapotan; Indriastuti, Rhesti; Satrio , Faisal Amri; Memili, Erdogan
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 1 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0401.220-224

Peningkatan populasi dan mutu ternak, khususnya sapi dan kerbau d ilakukan melalui program inseminasi buatan (IB). Angka kebuntingan hasil IB di Indonesia, relatif masih rendah, salah satunya diduga disebabkan oleh fertilitas pejantan. Program IB di Indonesia menggunakan semen beku yang diproduksi oleh Balai Inseminasi Buatan (BIB). Standar evaluasi semen bagi pejantan yang digunakan sesuai SNI masih mengacu pada konsentrasi, motilitas pra dan pasca thawing serta skor individu. Perkembangan terbaru menunjukkan bahwa parameter uji tersebut tidak lagi memadai. Protein dan gen pada spermatozoa dan seminal plasma pejantan dapat menjadi kandidat alat bantu “akurat” untuk menentukan fertilitas pejantan sesuai keberhasilan IB di lapangan. Policy brief ini diharapkan dapat menjadi bahan pertimbangan bagi Pemerintah dan BIB dalam p engembangan dan pengaplikasian penanda fertilitas berbasis molekuler sebagai alat bantu “akurat” dalam proses seleksi dan kebijakan pengafkiran pejantan sapi yang akan digunakan untuk IB. Dengan demikian, efisiensi penggunaan pejantan unggul di berbagai Ba lai Inseminasi Buatan, baik Nasional maupun Daerah di Indonesia dapat dioptimalkan.

Methods for assessing DNA fragmentation in bovine spermatozoa: A Mini-review of Indonesian research Satrio , Faisal Amri; Magfira; Karja , Ni Wayan Kurniani
ARSHI Veterinary Letters Vol. 9 No. 1 (2025): ARSHI Veterinary Letters - February 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.1.29-30

Artikel ini mengulas metode penelitian dan analisis fragmentasi DNA sperma sapi yang dilakukan di Indonesia. Tinjauan pustaka menggunakan Google Scholar mengidentifikasi 37 artikel oleh penulis Indonesia yang diterbitkan sejak 2018, dengan puncak delapan artikel pada 2023. Teknik utama yang digunakan meliputi pewarnaan Acridine Orange (17 artikel), kit Sperm-Bos-Halomax (12 artikel), pewarnaan Toluidine Blue (7 artikel), dan uji TUNEL (1 artikel), yang masing-masing menunjukkan variasi metodologis. Tinjauan ini memberikan referensi berharga bagi para pemangku kepentingan yang ingin mengembangkan peraturan standar untuk evaluasi sperma pada sapi.

PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Satrio, Faisal Amri; Karja, Ni Wayan Kurniani; Setiadi, Mohamad Agus; Kaiin, Ekayanti Mulyawati; Kurnia, Asep; Purwantara, Bambang
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.23487

This study aimed to determine the effect of age on bulls productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, 10 yo with high semen rejection (10 HR), and 10 yo with low semen rejection (10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of 10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in 10 yo groups. The 10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of 10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in 10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.

QUALITY OF CHILLED CANINE SEMEN IN TRIS-EGG YOLK EXTENDER SUPPLEMENTED WITH SERICIN Khye, Kim Chwin; Yusuf, Tuty Laswardi; Satrio, Faisal Amri; Karja, Ni Wayan Kurniani
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.17641

ABSTRACTThe objective of this research was to evaluate the quality of chilled canine semen in Tris-egg yolk (TEY) extenders containing different concentrations of sericin. Semen were collected from four dogs by massage method. Canine semen was collected using sterile urine pots and evaluated. Sperm-rich fractions were pooled and divided into four equal aliquots, which were then diluted with TEY extenders supplemented with different concentrations of sericin (0%, 0.1%, 0.25%, 0.5%). The diluted semen aliquots were preserved at 4 in sterile centrifuge tubes and were then evaluated for spermatozoa motility, viability, plasma membrane integrity and acrosome integrity every 12 hours up to 72 h. The TEY extenders supplemented with 0.25% and 0.5% sericin resulted in higher spermatozoa motility and viability at 72 h compared to other TEY extenders (P0.05). The integrity of plasma membrane and acrosome of spermatozoa showed no significant differences among the groups extenders at 72 h. In conclusion, sericin in concentration of 0.25% and 0.5% were able to prevent the motility and viability of canine spermatozoa after storage for 72 h.

Studi Kasus: Pendekatan Klinis dan Evaluasi Terapi dalam Penanganan Hematuria pada Sapi Perah di Kecamatan Cigedug, Kabupaten Garut: Case Study: Clinical Approach and Therapy Evaluation in Handling Hematuria in Dairy Cows in Cigedug District, Garut Regency Yusuf, Muchamad Ramadhan Ardhi; Hidayat, Yusep Saeful; Septiyani, Septiyani; Setyowati, Endang Yuni; Satrio, Faisal Amri
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 28 No 2 (2025): November 2025
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiiip.v28i2.42760

Background: The occurrence of various diseases in dairy cattle farms is the cause of low milk production in Indonesia. Hematuria or blood in the urine due to infectious and non-infectious diseases in dairy cattle is one of the causative factors. Objective: This case study aims to analyze the clinical approach in the diagnosis of hematuria in dairy cattle and evaluate the effectiveness of the therapy applied based on a case study in Cigedug District, Garut Regency. Method: This case study was conducted through anamnesis to obtain information, signals to identify the basic characteristics of dairy cattle, physical examination, and determination of diagnosis and prognosis, which were then followed by therapeutic management based on diagnostic findings. Results: A one-year-old female Friesian Holstein crossbreed dairy cow that had hematuria since one week before this examination appeared less active, had poor appetite, was trembling, had pale mucosa, a heart rate of 128 times per minute, a respiratory rate of 40 times per minute, and a temperature of 41.6 ºC. There was a hard tick infestation on the body and cage. After being injected with Tryponil® (diminazene aceturate and phenazone) via the intramuscular route, the cow's body temperature improved from 41.6 °C to 39.7 °C and hematuria was no longer visible 3 days after injection. Conclusion: A clinical approach that includes anamnesis, signaling, physical examination, and therapeutic management is effective in diagnosing and treating hematuria in dairy cows, as in the case study, where intramuscular administration of Tryponil® eliminated clinical symptoms and improved clinical condition within three days.

Co-Authors Agus Wijaya Aldridge, Mildred Elizabeth Anglin, William Wess Asep Kurnia Bambang Purwantara Blalock, Lauren Susanne Brown, Kipp Burkes, Briana Lashun Chen, Vincentius Owen Citra Ayu Lestari Cohn-White, Olivia Lucienne Dechert, Ed Ekayanti Mulyawati Kaiin Endang Yuni Setyowati Fearon, Jasmyn Elaine Franks, Brittany Renee Ghassani, Yumni Khairina Giles, Haleigh Margaret Greene, Ashley Lorraine Hadi Putra Rihansyah Hanby, Riley Dawn Haynes, Bethany Peyton Hidayat, Yusep Saeful Hilmi, Haitsam Muhammad Holliman, Anna Grace Indriastuti, Rhesti Intan Maria Paramita Kaiin, Ekayanti Mulyawati Khye, Kim Chwin Kim Chwin Khye Kirby, John Mark Klein, Austin William Kurnia, Asep Kutchy, Naseer Ahmad Lehmann, Courtney Ann Llyod, Grace Jennette Lore, Cody Triston M Agus Setiadi M Imron Magfira McMurray, Tyler B. Memili, Erdogan Miller, Hannah Marie Moody, Zachary Vinz Muhammad Elmanaviean Nadila, Nasywa Ni Wayan Kurniani Karja Oloan Parlindungan Palmer, Brianna Nicole Pansano, Lindsey Virginia Pardede , Berlin Pandapotan Pickle, Ryan Matthew Prianti, Nadiyah Dwi Putri, Alysa Gema Fatiah Puwantara, Bambang R. Iis Arifiantini Rahma, Hana Nayla Rutherford, Cobie Sanford, Katie Yvonne Sarasati Windria Schaeffer, Laura Marie Scott, Rachel Rebecca Seidl, Jeneva Ruth Septiyani Septiyani, Septiyani Smith, James Dean Sri Estuningsih Stepp, Hannah Faith Syamila, Nafira Hylma TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Wahyu Sri Wulandari Wendi Prameswari, Wendi Yohan Naim Nurul Fatonah Yuni Siswani Yusuf, Muchamad Ramadhan Ardhi

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