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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering)
Melani, Dewi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Materials Science & Nanotechnology Physics

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Bioactivity of Sweet Flag (Acorus calamus Linnaeus) Essential Oils against Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Melani, Dewi; Himawan, Toto; Afandhi, Aminudin
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.04

Abstract

The study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a com-pletely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and anti-feedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatog-raphy-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone. Keywords:  Acorus calamus, essential oils, mortality,  antifeedant,  Spodoptera lituraThe study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a completely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and antifeedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatography-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone.
High Potential of Liquid Smoke from Coconut Shell (Cocos nucifera) for Biological Control of Rice Bug (Leptocorisa oratorius Fabricius) Gama, Zulfaidah Penata; Purnama, Rizky Mulyo Adi; Melani, Dewi
Journal of Tropical Life Science Vol 11, No 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.11

Abstract

Rice bug (Leptocorisa oracorius F) is one of the most dangerous pests for paddy commodities (Oryza sativa) in the world. The use of liquid smoke was popular among local farmers to control rice bug. A coconut shell is the main source for making liquid smoke. This research aims to analyse rice bugs' mortality and antifeedant activity when dipped with coconut shell liquid smoke. The liquid smoke's effectiveness was measured by dipping paddy (5 grams in weight) that is previously soaked with coconut shell liquid for 20 seconds. The liquid smoke was prepared in various concentrations, ranging from 0% as a control; 0.50%; 0.75%; 1%; 1.25% and 1,50%. Observations were made at 24, 48, 72, 96, 120, 144 and 168 hours. Data analysis was performed by one-way ANOVA test, using SPSS program followed by a 0.05 Tukey test to determine the significance of the rice bug pest mortality. The study resulted that the mortality values from low concentrations to high concentrations were 40%, 46.67%, 60%, 70%, and 80% over seven days. The antifeedant percentage of liquid smoke increased from low concentrations to high concentrations respectively were 10.14%, 15.15%, 31.03%, 46.15%, and 68.88% during seven days. The concentration of liquid smoke that has the highest mortality was 1.50%. In conclusion, 1.50% of liquid smoke showed the highest percentage of mortality and antifeedant activity with 80% and 68.88%, respectively. In the future, it is hoped to develop this model for commercial consumption and reduce reliance on chemicals to control rice bugs. The use of pesticides can do more harm than good, especially to the environmental system.
Study of Distillation Method and Time on the Quality of Basil (Ocimum sanctum L.) Essential Oil Soekarno, Siswoyo; Hernanto, Setyawan Dwi; Purbasari, Dian; Melani, Dewi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 2 (2024): June 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i2.606-614

Abstract

"Basil" or basil has economic value both in fresh form, simplicia and essential oil. This research aims to evaluate the method and duration of distillation in making essential oil from basil. Two distillation methods, namely steam and boil distillation, are carried out with different distillation times (1 hour, 2 hours, and 3 hours). The parameters observed were physical characteristics which included oil volume, yield, pH, distillation rate, color, aroma and spots. The results show that the best distillation process time is 2 h for both distillation methods. Steam distillation for 2 hours produced the best essential oil of 1.1 ml, yield 0.11%, pH 5.78, distillation rate of 0.53 ml/h, pale yellow color, strong aroma, clean spots. Meanwhile, water boiled distillation for 2 h produced 1.2 ml of essential oil, 0.12% yield, pH 6.31, distillation rate of 0.60 ml/h, brown color, weak aroma, very spotty. Distillation of basil leaf essential oil using the steam method for 2 h was determined as the best treatment based on the highest essential oil yield, smallest pH, highest distillation rate, clearest color, most distinctive aroma, and cleanest stain. Keywords: Aroma, Basil leaves, Essential oil, Steam distillation, Yield.
Potential of Binahong Leaves (Anredera cordifolia Ten.) and Shallot Skin (Allium cepa L.) Extract as Biopesticides for Biological Control of Rice Bug (Leptocorisa oratorius F.) Lestari, Fenti Wahyu; Melani, Dewi; Gama, Zulfaidah Penata
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.8755

Abstract

The rice bug is a pest causing decrease rice production. Meanwhile, binahong leaves and shallot skin contain potential materials for making biopesticides. This research aimed to analyze potential of binahong leaves and shallot skin extract as biopesticides and toxicity tested against rice bug. The research was carried out using Completely Randomized Design, biopesticides were made in various concentrations, 0% as a control; 10; 20; 30; 40; and 50. The extract was calculated for yield, pH, and phytochemical screening. Then, biopesticide sprayed on the pests at 24, 48, 72, 96, 120, 144, and 168 hours to observe mortality, LC50, morphology, behavior, biotic, and data analysis using ANOVA followed 0.05 Tukey test. The results showed the extract has potential as a biopesticide with yield value 8,3 – 12; pH value 4,84 – 5,37; and positive secondary metabolite. Meanwhile, toxicity tests showed the highest mortality at concentration 50%, LC50 at 144th hours and 168th hours was 4,373 and 2,002. Besides that, biopesticides changes morphology and behavior pest. Observations abiotic factors showed that rice bug can survive in temperature range of 22,75 – 25oC and relative humidity of 77 - 84%. In the future, it hoped biopesticides can be commercialized to reduce dependence on chemical pesticides.
Co-Authors Aminudin Afandhi Dian Purbasari Hernanto, Setyawan Dwi Lestari, Fenti Wahyu Purnama, Rizky Mulyo Adi Siswoyo Soekarno Toto Himawan Zulfaidah Penata Gama