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Construction and Immunogenicity Testing of Salmonella, STM1 Vaccine Vector Expressing HIV-1 Antigen Bachtiar, Endang Winiati; Smooker, Peter; Coloe, Peter J
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.952 KB)

Objective of this study: to determine the ability of Salmonella enterica serovar Typhimurium STM1 as a delivery vehicle for the HIV p24 gene and HIV env gene. The STM1 delivery HIV-p24 vaccination was carried out in the form of a recombinant or a DNA vaccine whereas only a DNA vaccine was used for HIV env. Naked DNA vaccination was also tested and immune responses were evaluated following immunisation in mouse model. Results: vaccination cellular immune responses induced by recombinant p24 STM1 (STM1/pHly-p24) were greater than those elicited by the p24 DNA vaccine in STM1 (STM1/VR-p24), (but statistically not significance) than those induced by oral vaccination. However, IgA responses induced by oral vaccination with either a recombinant or DNA vaccine of p24 in STM1 are higher than those induced by IP vaccination. Conclusions: This result confirms</div><div>other studies that Salmonella was able to deliver HIV antigens to the immune system and induced specific immune responses to the HIV antigen.

Antigen Presentation Ability of Salmonella Carrying DNA Vaccine Model and MCP-3 gene Bachtiar, Endang Winiati; Smooker, Peter; Coloe, Peter J
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (953.754 KB)

The objective of this study is to determine the antigen presentation ability of a DNA vaccine model that is co-delivered with that of recombinant Salmonella enterica serovar Typhimurium (STM1) expressing chemokine macrophage chemotactic protein-3 (MCP-3). The DNA vaccine, pVROVA, was constructed by amplification of the ovalbumin coding region from sOVA-C1. Dendritic cells (DCs) were obtained from IL-4 and GMCSF stimulated mouse bone marrow stem cell. Cultured DCs were incubated with STM1 carrying a model ovalbumin gene (pVROVA). Furthermore, MHC class I antigen presentation of a dominant OVA peptide was assayed in vitro. The experiments were designed to determine the effect of co-delivering MCP-3 with that of ovalbumin in STM1. Our results show that a plasmid pROVA-carrying ovalbumin gene was succesfully constructed and sequence analysis of the ovalbumin-coding revealed an identity match of 100% with that of the chicken ovalbumin DNA sequences from the GenBank database. We also found that the presence of the MCP-3 encoding plasmid in STM1 or E. coli DH1 could increase the recovery of both STM1 and E. coli DH1 over those that carry the empty plasmids. Antigen presentation assay also indicates that MCP-3 can positively influence the presentation of ovalbumin. Conclusion: the infection of DCs by STM1-carrying DNA vaccine and MCP-3 results in an increase of processing and presentation of ovalbumin in vitro.Keywords : DNA vaccine, MCP-3, APC, Salmonella, Dendritic cells

Immunoglobulin-Y Effect On Protein Of Streptococcus Mutans Isolated From Caries And Caries-Free Subjects Aditiya Irwandi, Rizky; Winiati Bachtiar, Endang; Yuniastuti, Mindya
Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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The main microbial culprit in dental caries is Streptococcus mutans (S.mutans),virulence of which can be observed by its differential protein expression betweencaries and caries-free subjects.The success of Immunoglobulin-Y (IgY) antiS.mutans as a passive immunization agent in eliminating S.mutans has beenreported. The aim of this study is to analyze the effect of IgY anti S.mutans on theprotein expression of S.mutans isolated from caries and caries-free subjects. Eachdental plaque was collected by swabbing the buccal surface of the first lowerpermanent molar of caries and caries-free subjects. The plaques were thencultured on agar medium TYS20B. After 72 hours, the colonies from each of themwere cultured in liquid medium TYS Broth for 72 hour. Each collected bacteria(whether from caries or caries-free subjects) were grouped into control andexposure group. In exposure group, S.mutans was exposed by pre-incubated (forone hour at 37Â°C) IgY anti S.mutans for one hour at 37Â°C. Protein expression ofS.mutans was analyzed with SDS PAGE after the preparation of its antigen andBradford protein assay. Our result shows that S.mutans 41.3 kilodalton proteinexpression of caries subjects, are up-regulated in comparison to the control group.Meanwhile, the S.mutans 41.3 kilodalton protein expression of caries-freesubjects, are down-regulated in comparison to the control group. This studysuggests that IgY anti S.mutans up-regulates 41.3 kilodalton protein expression ofS.mutans in the caries subjects. However IgY anti S.mutans down-regulates 4.13kilodalton protein expression of S.mutans in the caries-free subjects.

Pertimbangan Penggunaan Implan Gigi pada Lansia Ananda, Nissia; Sulistyani, Lilies Dwi; Bachtiar, Endang Winiati
Insisiva Dental Journal Vol 6, No 1 (2017)
Publisher : Insisiva Dental Journal

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Saat ini terjadi perkembangan populasi lansia di Indonesia sehingga isu kesehatan lansia merupakan sesuatu yangesensial. Lansia adalah individu yang telah mengalami proses menua sehingga terjadi berbagai perubahan biologispada tubuhnya, sehingga berpengaruh terhadap penurunan fungsi organ. Kehilangan gigi adalah salah satu masalahyang umum pada lansia sehingga kebutuhan untuk pemasangan gigi tiruan merupakan hal penting yang perludiperhatikan masyarakat kelompok lansia. Implan gigi tiruan merupakan salah satu alternatif pengganti kehilangangigi yang memiliki banyak keuntungan dibandingkan gigi tiruan lain. Namun perlu disadari bahwa penggunaan implangigi memiliki pertimbangan-pertimbangan kondisi rongga mulut dan sistemik tertentu untuk menunjang keberhasilanperawatan. Pemahaman mengenai terjadinya proses menua dan hubungannya dengan pertimbangan penggunaanimplan sangat penting untuk diperhatikan oleh klinisi sebelum merencanakan perawatan, terutama berkaitan denganperubahan pada sistem pertahanan tubuh yang terjadi seiring proses menua.

EFFECT OF CORAL GONIOPORA IN COMPARISON WITH CORAL APATITE TOWARDS HUMAN DENTAL PULP STEM CELLS MINERALIZATION ACTIVITIES: EFEK CORAL GONIOPORA DIBANDINGKAN DENGAN CORAL APATITE TERHADAP AKTIVITAS MINERALISASI SEL STEM PULPA GIGI Rachmi Fanani Hakim; Endang Winiati Bachtiar; Nurtami Soedarsono
Dentika: Dental Journal Vol. 17 No. 1 (2012): Dentika Dental Journal
Publisher : TALENTA

Challenging approach in tissue engineering for dentin regeneration is focused upon the application of a scaffold on anopen pulp enabling odontoblast-like cells to grow into the scaffold and to convert them into dentin-like substance. Coralwas chosen as a scaffold because of its good biocompatibility and resorbability. The species of marine invertebratesexploited in medical applications are Members of Porites and Goniopora. Coral goniopora is most marine invertebratafound in Indonesia's marine. Coral apattite, an osteoconductive synthetic bone graft substitute material, is manufacturedby the hydrothermal conversion of the calcium carbonate skeleton of coral to hydroxyapattite in the presence ofammonium phosphate preserving the original porous structure which is similar to that of bone. The aim of study was toinvestigate the effect of Coral goniopora and coral apattite as a potential scaffold on dental pulp mineralization activity. Invitro DPSCs mineralization activity was measured by von Kossa staining for calcium deposit identification. The resultthat Coral apattite increased more calcium deposited identification than coral goniopora. Calcium deposited on dentalpulp stem cells are marker for mineralized dental pulp stem cells (DPSCs). Mineralized DPSCs are marker forodontoblast diferentiation and maturation. In conclusion, these observations demonstrated that co-cultured coral apattiteand DPSCs induced a better mineralization activity than those cultured with Coral goniopora.

The Role of Porphyromonas gingivalis Virulence Factors in Periodontitis Immunopathogenesis: Peran Faktor Virulensi Porphyromonas Gingivalis pada Imunopatogenesis Periodontitis) Septiwidyati, Tienneke Riana; Bachtiar, Endang Winiati
Dentika: Dental Journal Vol. 23 No. 1 (2020): Dentika Dental Journal
Publisher : TALENTA

Porphyromonas gingivalis is an anaerobic Gram-negative oral bacterium involved in the pathogenesis of periodontitis. Periodontitis is an infection that is characterized by damage to the supporting tissues of the teeth so that it can cause tooth loss if not given treatment. P. gingivalis locally can invade periodontal tissue and avoid host defense mechanisms. This bacterium has virulence factors which can cause deregulation of innate immune responses and inflammation in the host. The role of P. gingivalis virulence factors such as capsules, fimbriae, lipopolysaccharides, and gingipain in the pathogenesis of periodontitis will be discussed in this paper.

The Role of Porphyromonas gingivalis Virulence Factors in Periodontitis Immunopathogenesis: Peran Faktor Virulensi Porphyromonas Gingivalis pada Imunopatogenesis Periodontitis) Septiwidyati, Tienneke Riana; Bachtiar, Endang Winiati
Dentika: Dental Journal Vol. 23 No. 1 (2020): Dentika Dental Journal
Publisher : TALENTA

Porphyromonas gingivalis is an anaerobic Gram-negative oral bacterium involved in the pathogenesis of periodontitis. Periodontitis is an infection that is characterized by damage to the supporting tissues of the teeth so that it can cause tooth loss if not given treatment. P. gingivalis locally can invade periodontal tissue and avoid host defense mechanisms. This bacterium has virulence factors which can cause deregulation of innate immune responses and inflammation in the host. The role of P. gingivalis virulence factors such as capsules, fimbriae, lipopolysaccharides, and gingipain in the pathogenesis of periodontitis will be discussed in this paper.

Immunoglobulin-Y Effect On Protein Of Streptococcus Mutans Isolated From Caries And Caries-Free Subjects Aditiya Irwandi, Rizky; Winiati Bachtiar, Endang; Yuniastuti, Mindya
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 1, No 1 (2012)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v1i1.514

The main microbial culprit in dental caries is Streptococcus mutans (S.mutans),virulence of which can be observed by its differential protein expression betweencaries and caries-free subjects.The success of Immunoglobulin-Y (IgY) antiS.mutans as a passive immunization agent in eliminating S.mutans has beenreported. The aim of this study is to analyze the effect of IgY anti S.mutans on theprotein expression of S.mutans isolated from caries and caries-free subjects. Eachdental plaque was collected by swabbing the buccal surface of the first lowerpermanent molar of caries and caries-free subjects. The plaques were thencultured on agar medium TYS20B. After 72 hours, the colonies from each of themwere cultured in liquid medium TYS Broth for 72 hour. Each collected bacteria(whether from caries or caries-free subjects) were grouped into control andexposure group. In exposure group, S.mutans was exposed by pre-incubated (forone hour at 37Â°C) IgY anti S.mutans for one hour at 37Â°C. Protein expression ofS.mutans was analyzed with SDS PAGE after the preparation of its antigen andBradford protein assay. Our result shows that S.mutans 41.3 kilodalton proteinexpression of caries subjects, are up-regulated in comparison to the control group.Meanwhile, the S.mutans 41.3 kilodalton protein expression of caries-freesubjects, are down-regulated in comparison to the control group. This studysuggests that IgY anti S.mutans up-regulates 41.3 kilodalton protein expression ofS.mutans in the caries subjects. However IgY anti S.mutans down-regulates 4.13kilodalton protein expression of S.mutans in the caries-free subjects.

Construction and Immunogenicity Testing of Salmonella, STM1 Vaccine Vector Expressing HIV-1 Antigen Endang Winiati Bachtiar; Peter Smooker; Peter J Coloe
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Objective of this study: to determine the ability of Salmonella enterica serovar Typhimurium STM1 as a delivery vehicle for the HIV p24 gene and HIV env gene. The STM1 delivery HIV-p24 vaccination was carried out in the form of a recombinant or a DNA vaccine whereas only a DNA vaccine was used for HIV env. Naked DNA vaccination was also tested and immune responses were evaluated following immunisation in mouse model. Results: vaccination cellular immune responses induced by recombinant p24 STM1 (STM1/pHly-p24) were greater than those elicited by the p24 DNA vaccine in STM1 (STM1/VR-p24), (but statistically not significance) than those induced by oral vaccination. However, IgA responses induced by oral vaccination with either a recombinant or DNA vaccine of p24 in STM1 are higher than those induced by IP vaccination. Conclusions: This result confirms</div><div>other studies that Salmonella was able to deliver HIV antigens to the immune system and induced specific immune responses to the HIV antigen.

Antigen Presentation Ability of Salmonella Carrying DNA Vaccine Model and MCP-3 gene Endang Winiati Bachtiar; Peter Smooker; Peter J Coloe
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

The objective of this study is to determine the antigen presentation ability of a DNA vaccine model that is co-delivered with that of recombinant Salmonella enterica serovar Typhimurium (STM1) expressing chemokine macrophage chemotactic protein-3 (MCP-3). The DNA vaccine, pVROVA, was constructed by amplification of the ovalbumin coding region from sOVA-C1. Dendritic cells (DCs) were obtained from IL-4 and GMCSF stimulated mouse bone marrow stem cell. Cultured DCs were incubated with STM1 carrying a model ovalbumin gene (pVROVA). Furthermore, MHC class I antigen presentation of a dominant OVA peptide was assayed in vitro. The experiments were designed to determine the effect of co-delivering MCP-3 with that of ovalbumin in STM1. Our results show that a plasmid pROVA-carrying ovalbumin gene was succesfully constructed and sequence analysis of the ovalbumin-coding revealed an identity match of 100% with that of the chicken ovalbumin DNA sequences from the GenBank database. We also found that the presence of the MCP-3 encoding plasmid in STM1 or E. coli DH1 could increase the recovery of both STM1 and E. coli DH1 over those that carry the empty plasmids. Antigen presentation assay also indicates that MCP-3 can positively influence the presentation of ovalbumin. Conclusion: the infection of DCs by STM1-carrying DNA vaccine and MCP-3 results in an increase of processing and presentation of ovalbumin in vitro.Keywords : DNA vaccine, MCP-3, APC, Salmonella, Dendritic cells

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