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Machado, Maria Angela Naval
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Dentistry

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Exfoliative Cytology in the Oral Mucosa of Patients with Fanconi Anaemia: A Morphometric Approach Machado, Maria Angela Naval; D’Agulham, Anna Clara Duszczak; Chaiben, Cassiano Lima; Castro, Andrea Santos; Bonfim, Carmem Maria Sales Sales; de Lima, Antonio Adilson Soares
Journal of Dentistry Indonesia Vol. 24, No. 1
Publisher : UI Scholars Hub

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Patients with Fanconi anaemia (FA) are prone to develop squamous cell carcinomas at an early age. Exfoliativecytology can be used to detect changes to the health of the oral mucosa. Objective: The aim of this study was to evaluate morphometrical and morphological changes using exfoliative cytology and to analyse and quantify the proliferative activity using silver nucleolar organiser regions (AgNOR) in epithelial cells of the tongue in FA patients, after haematopoietic stem cell transplantation (HSCT). Methods: Oral smears were collected from the tongues of 20 FA children and adolescents after haematopoietic stem cell transplantation (FA) and 20 healthy children (C) using exfoliative cytology. The smears were stained using the Papanicolaou technique and silver impregnation. The cells were morphologically analysed and the nuclear area (NA), the cytoplasmic area (CA), and the nucleusto-cytoplasm area ratio (NA/CA) were calculated. Results: Mean values for the FA and C groups were: NA (71.85 and 55.21μm²; p< 0.01); CA (2127.48 and 1441.61μm²; p< 0.01); NA/CA (0.03 and 0.04; p< 0.01), respectively. A significant increase in the NA and CA for the FA group (p< 0.01) was seen, and an alteration in the NA/CA ratio. No morphological differences were found between the groups. Class I smears were predominant in both groups. No differences were found between the groups for the mean values of AgNORs per nucleus. Conclusion: This study suggests that morphological changes occurred in the oral epithelium cells of children and adolescents with Fanconi anaemia when subjected to HSCT.
Genotoxic and Cytotoxic Potential of Smoke Crack Cocaine on the Epithelium of the Human Oral Mucosa de Lima, Raphaela Cássia; Ferraz, Priscila; Chaiben, Cassiano Lima; Fernandes, Ângela; Grégio, Ana Maria Trindade; Machado, Maria Ângela Naval; Azevedo-Alanis, Luciana Reis; de Lima, Antonio Adilson Soares
Journal of Dentistry Indonesia Vol. 23, No. 2
Publisher : UI Scholars Hub

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Crack cocaine is an illicit drug derived from cocaine. It can produce some damages to the lungs and oral cavity. Objective: The aim of this study was to analyze the frequency of micronuclei and some nuclear alterations in epithelial cells of crack cocaine users. Methods: Oral smears were collected from clinically normal-appearing buccal mucosa exfoliative cytology of 30 individuals (15 crack cocaine users and 15 controls). Results: Crack cocaine users consumed about 3.8 grams per day and the time consumption of the drug was of 6.4 (+3.3) years. The prevalence of micronuclei, binucleated cells, broken egg cells, budding cells, picnosis, karyolysis, and karyorrhexis was determined. The frequencies of micronuclei for case and control groups were, respectively, 2.87 + 3.46 and 0.57 + 1.6 (p=0.018). No statistical difference was observed for binucleated cells, broken egg cells, budding cells, picnosis, and karyolysis. The frequency of karyorrhexis was significantly increased on crack cocaine users than controls (54.07 + 38.58 and 24.87 + 23.97, p=0.001). Conclusion: Smoke crack might have a cytotoxic and genotoxic effects to the oral mucosa due to increased frequency of micronuclei and karyorrhexis. Thus, individuals who used crack cocaine in the long term need to be frequently examined in order to prevent neoplastic transformation.
Co-Authors Azevedo-Alanis, Luciana Reis Bonfim, Carmem Maria Sales Sales Castro, Andrea Santos Chaiben, Cassiano Lima de Lima, Antonio Adilson Soares de Lima, Raphaela Cássia D’Agulham, Anna Clara Duszczak Fernandes, Ângela Ferraz, Priscila Grégio, Ana Maria Trindade