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CRYPTOCOCCAL ANTIGENEMIA IN HIV/AIDS PATIENTS USING LATERAL FLOW IMMUNOASSAY DETECTION AT Dr. SOETOMO GENERAL HOSPITAL SURABAYA Widjaja, Sajuni; Triyono, Erwin Astha; Kawilarang, Arthur Pohan; Rohiman, Abu
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 1 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.56 KB) | DOI: 10.20473/ijtid.v7i1.6311

Abstract

Cryptococcus  infection in HIV / AIDS patients results in cryptococcal meningitis, a major cause of subacute meningitis  with 100% mortality if not receiving appropriate antifungal therapy. An examination of cryptococcal antigen will provide risk information for patients who will experience cryptococcal meningitis. Better diagnosis in asymptomatic and symptomatic phases of cryptococcosis are key components to reduce morbidity and mortality. This study aims to determine the proportion of cryptococcal antigenemia in HIV / AIDS patients treated at Intermediate Treatment-Infectious Diseases Unit of Dr. Soetomo General Hospital Surabaya. Cryptococcal antigenemia was examined in HIV / AIDS patients with suspected Cryptococcus infection and CD4+ T cell lymphocyte count <200 cell /μl. The examination used a lateral flow assay diagnostic tool, a simple FDA(Food and Drug Administration)-approved immunochromatographic test system for detection of capsular polysccharide antigens of Cryptococcus species complex (Cryptococcus neoformans and Cryptococcus gattii) in blood. This test meets all of the World Health Organization ASSURED criteria (affordable, sensitive, specific, user friendly, rapid/robust, equipment-free, and delivered). Sensitivity and specifiticy of this method from serum are both 100%. There were 3 positive cryptococcal antigenemia from 41 serum HIV / AIDS patients with suspected cryptococcus infection at Intermediate Treatment- Infectious Diseases Unit of Dr. Soetomo General Hospital Surabaya. All of these patients were male aged over 36 years, had CD4+ T cell lymphocytes <100 cell /μl and had never received antiretroviral therapy before. The proportion of cryptococcal antigenemia in HIV / AIDS patients with suspected Cryptococcus infection at Intermediate Treatment-Infectious Diseases Unit of Dr. Soetomo General Hospital Surabaya was 7.32%.
PERBANDINGAN EFEKTIVITAS EKSTRAK BAWANG PUTIH TUNGGAL (Allium sativum L.) DAN BAWANG PUTIH MAJEMUK (Allium sativum) TERHADAP Klebsiella pneumoniae SECARA IN VITRO Widjaja, Sajuni; Ikawaty, Risma; Avista Diapermata
Medika Alkhairaat: Jurnal Penelitian Kedokteran dan Kesehatan Vol 7 No 01 (2025): April
Publisher : Fakultas Kedokteran Universitas Alkhairaat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/ma.v7i01.266

Abstract

ABSTRAK Infeksi masih menjadi salah satu masalah kesehatan yang banyak dijumpai. Salah satu bakteri penyebab penyakit infeksi adalah Klebsiella pneumoniae. Namun bakteri tersebut sudah mulai resisten sehingga diperlukan alternatif pengobatan lain. Masyarakat Indonesia erat dengan penggunaan herbal termasuk bawang putih. Bawang putih memiliki senyawa aktif yang bersifat antibakteri. Dua jenis bawang putih yang sering digunakan saat ini adalah bawang putih tunggal (Allium sativum L.) dan majemuk (Allium sativum). Penelitian bertujuan untuk mengetahui perbandingan efektivitas ekstrak bawang putih tunggal dan majemuk sebagai antibakteri terhadap Klebsiella pneumoniae.. Metode Penelitian melibatkan uji efektivitas antibakteri dilakukan dengan metode difusi cakram (Kirby-Bauer) pada ekstrak bawang putih tunggal dan majemuk dengan konsentrasi 6,25% ; 12,50% ; 25% ; 50% ; 100%. Hasil penelitian menunjukkan bahwa ekstrak bawang putih tunggal menghasilkan rata-rata diameter zona hambat 1mm; 1,75mm; 2mm; 2,75mm; 4,12mm sedangkan ekstrak bawang putih majemuk menghasilkan rata-rata diameter zona hambat 1mm; 1,75mm; 2mm; 2,75mm; 4,12mm pada konsentrasi 6,25%; 12,50% ; 25% ; 50% ; 100%. Dari pengamatan makro dan hasil uji Mann-Whitney, tidak didapatkan perbedaan signifikan (p>0,05) antara bawang putih tunggal dan majemuk. Sebagai kesimpulanbawang putih tunggal dan majemuk memiliki efek antibakteri namun tidak terdapat perbedaan signifikan di antara keduanya. ABSTRACT Infectious diseases are still one of the most common health problems. One of the infectious bacterias is Klebsiella pneumoniae. These bacteria have started to become resistant so other alternative treatments are needed. Indonesian people often use herbs including garlic. Garlic has active antibacterial compounds. Two types of garlic that are often use are single and multi bulb garlic. There has been no study comparing single and multi bulb garlic to Klebsiella pneumoniae. To compare the effectiveness of single bulb garlic (Allium sativum L.) and multi bulb garlic (Allium sativum) extracts as antibacterial against Klebsiella pneumoniae. This research is laboratory experimental research. Antibacterial effectiveness test using disc diffusion method on single and multi bulb garlic extracts with concentration of 6.25% ; 12.50% ; 25% ; 50% ; 100%. Single bulb garlic extract produced average inhibition zone diameter 1mm; 1.75mm; 2mm; 2.75mm; 4.12mm while multi bulb garlic extract produced 1mm; 1.75mm; 2mm; 2.75mm; 4.12mm at concentration 6.25% ; 12.50% ; 25% ; 50% ; 100%. From macro observations and Mann Whitney test, there was no significant difference (p>0.05) between single and multi bulb garlic. Single and multi bulb garlic have no significant difference as antibacterial.
Efektivitas Vaksin Cacar Monyet Widjaja, Sajuni
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 10 No 2 (2023)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56710/wiyata.v10i2.653

Abstract

Latar Belakang: Dunia kesehatan prihatin dengan timbulnya wabah Cacar Monyet, begitu juga dengan Indonesia. Pada tengah Tahun 2022 Kementrian Kesehatan Indonesia mengumumkan seorang pria terjangkit penyakit ini sehingga pemerintah mulai menggalakkan upaya penanggulangan wabah Cacar Monyet  melalui penggunaan vaksin. Tujuan: Menganalisa efektifitas vaksin Cacar Monyet sebagai usaha pencegahan penyebaran penyakit Cacar Monyet. Metode: Literature review dengan metode naratif yang mengkaji dan menganalisis hasil penelitian yang terkait dengan pemberian vaksin kaitannya dengan penyakit Cacar Monyet melalui pencarian literatur baik nasional atau international dengan menggunakan database PubMed dan Google Cendekia. Hasil: Penggunaan vaksin mampu memberikan manfaat positif pada kekebalan individu, masyarakat dan komunitas. Simpulan: Vaksin efektif sebagai salah satu cara pencegahan Cacar Monyet.