Article Per Year (5 Year)
p-Index From 2020 - 2025
0.659
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Makara Journal of Science Jurnal Kedokteran Hewan
Noviana, Rachmitasari
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Veterinary

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Development and Optimization of an Immunoassay for the Detection of Antibodies Against SARS-CoV-2 with In-house Recombinant RBD Protein Ratu, Safira Pinaka Pramestika; Mariya, Silmi; Noviana, Rachmitasari; Saepuloh, Uus; Darusman, Huda Salahudin
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

COVID-19 caused by SARS-CoV-2 poses a major threat to the global community, particularly in Indonesia. Countermeasures to prevent the spread of this disease have also been implemented, including the implementation of a vaccination program. An immunoassay technique that can be used to analyze antibodies that might develop following vaccination is the indirect enzyme-linked immunosorbent assay (ELISA). We produced the recombinant spike protein used in this study. The optimization comprised adjusted concentrations of spike recombinant protein (5 and 10 ng/mL), blocking agent (2.5% and 5%), and conjugate (1:1000 and 1:5000). The optimal conditions in this study included a spiked concentration of 10 ng/mL, a blocking agent concentration of 5%, sample dilution of 1:33, and a conjugate concentration of 1:1000. The intra-assay value of this optimized indirect ELISA was 7.3, and the inter-assay value was 5.3. The commercial MyBioSource kit and immunodiagnostic were utilized as a reference in the T-test, with P-values of 0 and 0.313, indicating that the recombinant protein in-house ELISA kit in this study demonstrated the same ability as the commercial immunodiagnostic kit in detecting SARS-CoV-2 antibodies, allowing it to be used for post-vaccination efficacy evaluation.
RELIABILITY OF AMYLOID DETECTION IMMUNOASSAY KITS FOR ALZHEIMERS DISEASE SCREENING: A PRELIMINARY STUDY Putri, Indah Aprianti; Ningrum, Emilna Mega; Noviana, Rachmitasari; Mariya, Silmi; Saepuloh, Uus; Retnani, Elok Budi; Hamdan, Muhammad; Nugraha, Jusak; Darusman, Huda Shalahudin
Jurnal Kedokteran Hewan Vol 18, No 3 (2024): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i3.31109

Abstract

Alzheimers Disease is a progressive neurodegenerative disease that damages the brain and is part of dementia. Alzheimers dementia is caused by various factors that until now the proper therapy is uncertain. Kit production as a diagnostic tool to support the development of screening for Alzheimers disease is a strategic effort. Screening based on detection with Enzyme-Linked Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a specific antibody material or monoclonal antibody to the A42 peptide. This study aimed to research the reliability of the amyloid detection kit which was developed as an alternative screening test for Alzheimers disease compared to commercially available kits. Based on the results and interpretation of inter and intra-assay coefficients, the in-house ELISA kit has comparable A42 detection to the commercially available ELISA kit
Development of Biotin and Horseradish Peroxidase Labelling against Monoclonal Antibody Amyloid-β42 and Its Application on Enzyme-Linked Immunosorbent Assay Fiero, Nur Rizky; Saepuloh, Uus; Darusman, Huda Shalahudin; Noviana, Rachmitasari
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12528

Abstract

Alzheimer disease is one of the causes of dementia that could make the patient lose their memories until they unable to do normal activities. It is caused by accumulation of protein in the brain called Amyloid-β42 (Aβ42), that often used as biomarker for alzheimer by enzyme-linked immunosorbent assay (ELISA). To increase the sensitivity and accuracy, capture antibodies are conjugated with labels such as Horseradish Peroxidase (HRP) and Biotin. However, the two conjugates also differ in sensitivity and specificity, therefore this research is to find which is more optimum and stable for ELISA usage. The molecular mass of the conjugated antibodies was characterized with SDS-PAGE, result shows the pre-conjugated antibody has 150 kDa bands, conjugated antibody with labels is heavier than the pre-conjugated due to the addition of each label. Conjugated antibody tested with tetramethylbenzidine (TMB), then analyzed by ELISA reader. The measurement precision decided with %CV value with results shows dillution from 10-1 to 10-4 has %CV less than 10%, while Biotin is from 10-1 to 10-5. Optimization done by determining fixed antigen with the conjugated antibody, the optimal concentration for HRP is 10-3 while biotin is at 10-4. Stability of the conjugated antibody was also determined, HRP measurement started to unstable around second month and third month, while biotin start to unstable around fourth month, this shows the biotin is more stable than the HRP.
Co-Authors Elok Budi Retnani Fiero, Nur Rizky Huda Shalahudin Darusman Jusak Nugraha Muhammad Hamdan Ningrum, Emilna Mega Putri, Indah Aprianti Ratu, Safira Pinaka Pramestika SILMI MARIYA UUS SAEPULOH