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EXPLORATION OF BIOACTIVE COMPOUNDS OF RAMBUSA (Passiflora foetida L.) ROOT EXTRACT FROM EAST KALIMANTAN COAL RECLAMATION LAND AS ANTIOXIDANT Meray, Nishia Waya; Utami, Indah Woro; Nurazizah
Jurnal Kimia Riset Vol. 9 No. 2 (2024): December
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v9i2.63620

Abstract

The rambusa plant (Passiflora foetida L.) is a species utilized as a cover crop due to its capacity to undergo a natural regeneration process in the context of former coal mining operations in East Kalimantan. Rambusa plants have many phytochemical properties, one of which is as an antioxidant. Nevertheless, there is currently a paucity of scientific data regarding the potential antioxidant properties of bioactive compounds in Rambusa roots utilized as cover crop plants. The objective of this study is to investigate the composition of bioactive compounds in the root extract of Rambusa plants that are cultivated on land previously utilized for coal mining in East Kalimantan, with a particular focus on their antioxidant properties. In this study, the exploration was carried out by conducting phytochemical tests using three types of solvents, namely ethanol, ethyl acetate and n-hexane and antioxidant activity tests using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method on the extracted samples. Phytochemical screening results showed the presence of alkaloid, phenolic, flavonoid, terpenoid and saponin bioactive compounds in ethanol extract; alkaloid, phenolic and steroid bioactive compounds in ethyl acetate extract. At the same time, alkaloids and steroids are contained in the n-hexane extract. The DPPH test carried out on the three extracts showed very strong antioxidant activity with IC50 values respectively for ethanol, ethyl acetate and n-hexane extracts are 6.55 ppm, 3.51 ppm and 28.71 ppm. Based on previously reported antioxidant activity data, the antioxidant activity of Rambusa (Passiflora foetida L.) roots growing on coal reclamation land is proven to have much higher activity compared to Rambusa plants growing on fertile land. Thus, the root of the Rambusa plant (Passiflora foetida L.) has enormous potential as an antioxidant and natural medicinal raw material.
Comparison of Characteristics and Antibacterial Activity of Mandai Cempedak Vinegar with Variations in Fermentation Methods Utami, Indah Woro; Sapri, Sapri; Meray, Nishia Waya; Canrika, Rezia Elok
Borneo Journal of Pharmacy Vol. 8 No. 3 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i3.9140

Abstract

Mandai, a traditional fermented food product from East Kalimantan, is derived from the edible mesocarp of the cempedak (Artocarpus champeden) fruit. This ancestral fermentation technique, passed down through generations, is known to produce lactic acid bacteria (LAB) with potential probiotic properties. This study aimed to characterize LAB isolated from mandai vinegar and evaluate their antibacterial activity under two distinct fermentation conditions: a defined starter culture of Lactobacillus casei strain Shirota and spontaneous fermentation in a 10% NaCl solution. The latter, referred to as salt fermentation, yielded three bacterial colonies, while the starter culture method produced four. All isolates from both methods shared similar morphological characteristics, including a rod shape, white coloration, entire margins, and convex surfaces. Further analysis confirmed they were Gram-positive, catalase-negative, and indole-negative, with the ability to ferment various carbohydrates. While all LAB isolates demonstrated generally low antibacterial activity against Escherichia coli ATCC 25922, isolate CML 1 exhibited the highest antibacterial potential. These findings highlight the potential of mandai vinegar as a source of functional LAB, warranting further investigation into its applications as a functional food ingredient.
ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT (BAL) DARI FERMENTASI STATER (Lactobacillus casei) PADA CUKA MANDAI CEMPEDEK (Artocarpus champeden) Rezia Elok Canrika; Utami, Indah Woro; Retno, Eka Kumala
PHARMACIA Vol. 2 No. 1 (2024): Pharmacy
Publisher : Program Studi Farmasi Universitas Mulia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mandai merupakan salah satu olahan makanan fermentasi dari kulit cempedak (Artocarpus champeden). Namun bagian dari mandai yang belum dimanfaatkan yakni cuka mandai yang dapat dibuat menjadi bahan pangan fungsional. Penelitian ini bertujuan untuk menentukan isolat dan mengetahui karakteristik dari bakteri asam laktat dari hasil fermentasi stater (Lactobacillus casei) pada cuka mandai cempedak. Metode penelitian ini menggunakan desain eksperimental dengan analisis data berupa analisis data dekskriptif. Fermentasi mandai ditambahkan stater 4% kemudian diambil sampel cuka pada hari ke-14 untuk di isolasi dengan Media MRS Agar dan dikarakterisasi. Dari hasil isolasi diperoleh empat koloni yang dapat dikatakan sebagai isolat dari bakteri asam laktat cuka mandai cempedak atau BAL CM dengan kode BAL CM1, BAL CM2, BAL CM3 dan BAL CM4. Keempat isolat tersebut memiliki karakteristik morfologi yang sama yakni berwarna putih dan tepi utuh dengan bentuk isolat yakni dua isolat bulat dan dua isolat tidak beraturan. Pada empat isolat tersebut menunjukan hasil berupa gram positif, berbetuk batang, uji katalase negatif tidak terbentuknya gelembung atau gas dan uji indol negatif tidak terbentuknya warna merah atau cincin merah. Keempat isolat tersebut diduga sebagai bakteri asam laktat genus Lactobacillus.
IDENTIFIKASI KANDUNGAN BORAKS, FORMALIN DAN CEMARAN MIKROBA PADA MIE BASAH DI KOTA BALIKPAPAN Utami, Indah Woro; Retno, Eka Kumala; Meray, Nishia Waya; Sapri, Sapri
INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL Vol 8, No 2 (2023)
Publisher : Fakultas Farmasi Universitas 17 Agustus 1945 Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52447/inrpj.v8i2.7214

Abstract

Mie basah merupakan bahan pangan yang banyak diminati oleh masyarakat Indonesia. Mie basah banyak diproduksi oleh industri rumah tangga dengan skala kecil. Mie basah dijual bebas di pasar tradisional dalam bentuk kemasan sederhana, basah dan dijual terbuka. Penelitian ini bertujuan untuk mengidentifikasi adanya kandungan formalin, boraks dan cemaran mikroba pada mie basah yang beredar di pasar tradisional di Kota Balikpapan. Metode pengujian formalin menggunakan pereaksi KMnO4 dan spektrofotometer UV-VIS, pengujian boraks menggunakan kertas turmeric dan uji cemaran mikroba menggunakan metode Total Plate Count (TPC). Total sampel mie basah yang didapatkan adalah 6 sampel dari 7 pasar tradisional di Kota Balikpapan. Hasil uji formalin dan boraks dianalisis secara kualitatif sedangkan data cemaran mikroba dianalisis secara kuantitatif dan membandingkan hasilnya dengan SNI. Hasil identifikasi menunjukkan bahwa seluruh sampel mie basah tidak terdapat campuran boraks namun terdapat satu yang terindikasi mengandung formalin. Hasil identifikasi cemaran mikroba menunjukkan mie basah mengandung mikroba dibawah ambang batas maksimal 1 x 106 koloni/gram dan dinyatakan memenuhi syarat mutu mie basah SNI 2987:2015. 
Evaluation of Antimicrobial Properties of Passiflora foetida Root Extract Sourced from Rehabilitated Coal Mining Sites in East Kalimantan Utami, Indah Woro; Sapri, Sapri; Meray, Nishia Waya; As'ari, As'ari
Sciences of Pharmacy Volume 4 Issue 4
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0404434

Abstract

Utilizing cover crops like Passiflora foetida L. (rambusa) can mitigate significant environmental issues in post-coal mining terrain rehabilitation. Plants thriving in extreme environments are known for producing high levels of secondary metabolites with significant biochemical activity. This study sought to assess the antifungal and antibacterial effects of P. foetida root extracts derived from rehabilitated coal mine soil in East Kalimantan. The roots were macerated with solvents of differing polarity (ethanol, ethyl acetate, and n-hexane) for extracting specific fractions. Utilizing the Kirby-Bauer disc diffusion method, the antifungal efficacy was assessed against Candida albicans, Candida tropicalis, and Candida lipolytica at a 60% concentration. Conversely, the antibacterial efficacy was assessed against Shigella dysenteriae, Streptococcus mutans, and Cutibacterium acnes at a 10% concentration. The findings revealed that the fraction consisting of ethyl acetate demonstrated the most potent and extensive antibacterial efficacy. The ethanol extract and ethyl acetate fraction exhibited significant antifungal activity, particularly against C. tropicalis, with inhibitory zones that were similar to those of the positive control, fluconazole. The treatments exhibited significant differences, as confirmed by statistical analysis (ANOVA and Tukey's HSD test). Studies indicate that that P. foetida roots, particularly those from harsh settings, are a significant source of antimicrobial chemicals, with the semi-polar ethyl acetate fraction being the most promising for further development as a natural antibacterial and antifungal agent.