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ANALYSIS OF HUMOR LANGUAGE IN “NGENEST THE MOVIE” COMEDY SCENE Natanael, Christian; Annita, Annita
VCD (Journal of Visual Communication Design) Vol. 7 No. 1 (2022): Journal of Visual Communication Design VCD
Publisher : Universitas Ciputra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/vcd.v7i1.2902

Abstract

Humor language is verbal humor created through words, the meaning of words, and jargon. Directors use language humor to build comedy scenes, introduce the characters, or give the impression of a scene. However, sometimes, excessive techniques will be insufficient for the scene itself. Therefore, this study aims to find how language humor in comedy scenes in a film is overused and eventually dries the comedy. Research material was selected from a comedy movie entitled "Ngenest," produced in 2015, directed by Ernest Prakasa, who has a comical background and often uses comedic techniques in his films. The research method chosen is qualitative, with content analysis to identify the value of integrated humor and scenes in the movie "Ngenest.” Keywords: humor, humor language, scene, comedy
Adipose-Derived Mesenchymal Stem Cell (AD-MSC)-Like Cells Restore Nestin Expression and Reduce Amyloid Plaques in Aluminum Chloride (AlCl3)-Driven Alzheimer's Rat Models Annita, Annita; Revilla, Gusti; Ali, Hirowati; Almurdi, Almurdi
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.387

Abstract

Background: Alzheimer's disease (AD) is a neurodegenerative disorder with a significant burden on public health, and current treatments offer limited efficacy. This study investigated the effectiveness of adipose-derived mesenchymal stem cells (AD-MSCs) on the expression of the nestin gene and amyloid plaque in an aluminum chloride (AlCl3)-driven Alzheimer's rat model.Materials and methods: AD-MSCs were characterized using flow cytometry. Adult male Wistar rats were treated with/without AlCl3 and injected with/without AD-MSCs. After 5 days of AlCl3 ingestion and 4 weeks of subsequent AD-MSCs intraperitoneal injection, behavioral and molecular assessments were conducted. The Y-maze alternation test was used to test spatial learning of rats. Nestin gene expression was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The presence of amyloid plaque in the cortex and the hippocampus was evaluated through Congo red staining.Results: AD-MSC-like cells expressed the MSC markers CD90, CD73 and CD105. The Y-maze alternation result for rats treated with AlCl3 and AD-MSC-like cells was significantly higher compared with rats treated with AlCl3 only. Nestin gene expression was significantly higher in rats treated with AlCl3 and AD-MSC-like cells compared to those treated with AlCl3 only. After AD-MSC-like cells treatment, the Congo red staining results of rat’s cortex and hippocampus were significantly decreased.Conclusion: The findings suggest that AD-MSC-like cells possess therapeutic potential in restoring neural plasticity, amyloid plaque clearance and warrant further investigation for AD treatment. This study contributes to the emerging field of stem cell therapy for neurodegenerative diseases by highlighting the promise of AD-MSCs.Keywords: Alzheimer's disease, adipose-derived mesenchymal stem cells, neural plasticity, congo red staining, stem cell therapy
KARAKTERISASI DAN IDENTIFIKASI MOLEKULER BAKTERI ASAM LAKTAT ASAL IKAN RINUAK (PSILOPSIS SP) SEBAGAI KANDIDAT PROBIOTIK HALAL DARI DANAU MANINJAU SUMATERA BARAT Annita, Annita; Zahra, Eliza; Prima, Heppy Setya
Jurnal Kesehatan Medika Saintika Vol 16, No 1 (2025): Juni 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i1.2976

Abstract

Lactic Acid Bacteria (LAB) have recently become one of the main topics of discussion in the fields of health, food industry, science, animal husbandry, agriculture. LAB plays an important role in efforts to reduce stunting through various mechanisms related to intestinal health and nutrient absorption. One source of LAB is fermented Rinuak fish (Psilopsis sp) from Lake Maninjau, West Sumatra, Indonesia, which is isolated from probiotic bacteria which have the potential to act as antimicrobials, but must be ensured that they are halal in accordance with Islamic religious teachings. The aim of this study was to examine the antimicrobial potential of probiotic LAB isolated from Rinuak fish (Psilopsis sp). The research methods carried out were as follows: isolation and characterization of Rinuak fish (Psilopsis sp) LAB DNA, followed by assessment of the antimicrobial activity of the raw bacteriocin supernatant. Then, 16S rRNA was used to assess LAB isolate species that potentially had antimicrobial activity. The results showed that 3 samples of Rinuak fish (Psilopsis sp), After testing its ability to kill pathogenic bacteria, it was found that IR1 isolate had the greatest antimicrobial activity against Escherichia coli ATCC 0157 (30..27 mm), Staphylococcus aureus ATCC 25923 (25.65 mm) and Listeria monocytogenes CFSAN 004330 (18.65 mm) while the diameter of the inhibition zone by the supernatant of lactic acid bacteria Crude bacteriocin against Escherichia coli ATCC 0157 was 18.87 mm, Staphylococcus aureus ATCC 25923 was 22.67 mm while Listeria monocytogenes CFSAN 004330 did not form an inhibition zone. The results of molecular identification using 16S rRNA showed that the isolated lactic acid bacteria were 99.64% similar to those isolated from Rinuak fish (Psilopsis sp) with Lentilactobacillus buchneri subsp. silagei CD034 strain 17-16, which has antimicrobial potential against pathogenic bacteria. Lentilactobacillus buchneri subsp. silagei CD034 strain 17-16 can be used as an antidiarrheal and antityphoid drug in humans as well as a natural preservative in food that is guaranteed halal
Exploring the Effects of Bone Marrow Mesenchymal Stem Cells on Amyloid Plaque Reduction in a Rat Model of Alzheimer's Disease Annita, Annita; Revilla, Gusti; Ali, Hirowati; Almurdi, Almurdi
Majalah Kedokteran Andalas Vol. 47 No. 1 (2024): MKA Januari 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i1.p79-86.2024

Abstract

Objective: This study aimed to evaluate the therapeutic potential of Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) in mitigating amyloid plaque formation in Alzheimer's disease induced by Aluminum Chloride (AlCl3) in rats. Methods: Rats were divided into three groups: negative control, positive control (AlCl3-induced), and treatment (AlCl3 + BM-MSCs). Cognitive function was assessed five days post-induction, and amyloid plaques were quantified using Congo Red staining in the cortex and hippocampus. Results: A significant reduction in amyloid plaque was observed in rats treated with BM-MSCs. The proportion of stained areas decreased from 1.88 to 1.73 in the cortex and from 1.61 to 1.47 in the hippocampus, compared to AlCl3-only controls. Conclusion: BM-MSCs demonstrated a moderate but statistically significant reduction in amyloid plaque in a rat model of Alzheimer's disease, indicating their therapeutic potential.
Pengelolaan Sampah Organik Kulit Jeruk Manis menjadi Sabun Mandi Cair: Solusi Ramah Lingkungan dan Pemberdayaan Ekonomi Ibu Rumah Tangga Yulia, Inelvi; Annita, Annita; Haq, Ruqaya Annisa Nurul
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 8, No 12 (2025): Volume 8 No 12 (2025)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v8i12.22646

Abstract

ABSTRAK Kulit jeruk manis merupakan salah satu limbah yang belum dimanfaatkan dan dianggap tidak bernilai. Padahal kulit jeruk manis mengandung senyawa bioaktif dan aroma yang baik berpotensi untuk dijadikan bahan dasar pembuatan sabun. Pemanfaatan limbah ini tidak hanya berpotensi mendukung upaya pengolahan sampah ramah lingkungan, tetapi juga berpeluang mensejahterakan ekonomi masyarakat. Tujuan kegiatan pengabdian ini adalah untuk memberikan pelatihan pembuatan sabun mandi cair dengan penambahan ekstrak kulit jeruk manis menjadi produk ramah lingkungan serta mendorong pemberdayaan ekonomi ibu rumah tangga di kelurahan Limau Manis, Kota Padang. Metode yang digunakan. Metode yang digunakan berupa pendekatan partisipatif melalui sosialisasi, pelatihan teknis, pendampingan pembuatan sabun cair dari kulit jeruk manis, serta pemantauan perkembagan masyarakat setelah pelatihan. Hasil kegiatan menunjukkan ibu rumah tangga mengalami peningkatan pengertahuan tentang pengolahan limbah kulit jeruk manis serta keterampilan dalam memproduksi sabun mandi cair. Produk yang dihasilkan memiliki aroma khas jeruk, busa yang cukup stabil, dan diterima baik oleh peserta. Selain itu, mitra termotivasi untuk mengembangkan usaha kecil berbasis produk sabun alami, sehingga membuka peluang ekonomi tambahan. Pengolahan limbah kulit jeruk manis menjadi sabun cair terbukti memberikan manfaat ganda, yaitu sebagai solusi ramah lingkungan dalam mengurangi sampah organik dan sebagai sarana pemberdayaan ekonomi ibu rumah tangga melalui keterampilan wirausaha baru. Kata Kunci: Limbah organik, Kulit Jeruk Manis, Sabun Mandi Cair, Ibu Rumah Tangga. ABSTRACT Sweet orange peel is a waste product that is underutilized and considered worthless. However, sweet orange peel contains bioactive compounds and a pleasant aroma, making it a potential raw material for soap production. Utilizing this waste not only supports environmentally friendly waste management efforts but also offers the potential to improve the community's economic well-being. The purpose of this community service activity is to provide training in making liquid soap by adding sweet orange peel extract to create an environmentally friendly product and to encourage the economic empowerment of housewives in Limau Manis sub-district, Padang City. The method used is a participatory approach through outreach, technical training, mentoring in making liquid soap from sweet orange peel, and monitoring community development after the training. The results of the activity show that housewives experienced an increase in knowledge about processing sweet orange peel waste and skills in producing liquid soap. The resulting product has a distinctive citrus aroma, a fairly stable foam, and is well received by participants. In addition, partners are motivated to develop small businesses based on natural soap products, thereby opening up additional economic opportunities. Processing sweet orange peel waste into liquid soap has been proven to provide dual benefits, namely as an environmentally friendly solution in reducing organic waste and as a means of economic empowerment for housewives through new entrepreneurial skills. Keywords: Organic Waste, Sweet Orange Peel, Liquid Bath Soap, Housewives