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All Journal Jurnal Nukleus Peternakan COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Animal Agricultura
Nalley, W. Marlene
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Industrial & Manufacturing Engineering Social Sciences Veterinary

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Perbandingan Kualitas Spermatozoa Babi Landrace dalam Pengencer Dasar Natrium Chloride Fisiologis dengan Level Kuning Telur yang Berbeda Ina Mato, Sefania; Nalley, W. Marlene; Mata Hine, Thomas; Marawali, Aloysius
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 4 No. 1 (2024): COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/comserva.v4i1.1292

Abstract

Penelitian ini dilakukan dengan tujuan untuk mengetahui level optimal kuning telur (KT) dalam pengencer natrium chloride fisiologis (NaClF) untuk preservasi spermatozoa babi landrace. Materi penelitian adalah semen yang berkualitas baik (motilitas spermatozoa ?70%, abnormalitas spermatozoa <20%) yang ditampung dari dua ekor babi jantan landrace, yang berumur ± dua tahun. Rancangan yang digunakan adalah rancangan acak lengkap yang terdiri dari enam perlakuan, yakni: P0: NaClF, P1: NaClF+5% KT, P2: NaClF+10% KT, P3: NaClF+15% KT, P4: NaClF+20% KT, P5: NaClF25% KT. Setiap perlakuan mendapat ulangan lima kali. Variabel yang diukur dalam penelitian ini adalah motilitas, viabilitas, abnormalitas, dan daya tahan hidup spermatozoa. Hasil penelitian ini menunjukkan bahwa hingga jam ke-24 preservasi, penambahan KT 5-25% ke dalam pengencer NaClF menghasilkan motilitas dan daya tahan hidup spermatozoa yang lebih tinggi daripada kontrol (P<0,05), namun untuk variabel viabilitas dan abnormalitas spermatozoa menunjukkan pengaruh yang tidak signifikan (P>0,05). Disimpulkan bahwa level optimal KT dalam pengencer NaClF untuk preservasi spermatozoa babi landrace adalah 20%
PENGARUH LEVEL ZINK DALAM PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SEMEN CAIR SAPI ANGUS (The effect of zinc level in the tris egg yolk extender on the quality of angus liquid semen) Siena, Sofia M.S.; Hine, Thomas Mata; Nalley, W. Marlene; Marawali, Aloysius
JURNAL NUKLEUS PETERNAKAN Vol 11 No 1 (2024): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v11i1.8346

Abstract

Efforts to improve cattle productivity can be done by using artificial insemination (AI) technology. Many factors determine the success of AI, one of which is the diluent used in semen preservation. The purpose of this study was to determine.the effect.of zinc level in.tris-egg yolk.diluent (T-EY) on the.quality of liquid semen of Angus bull. Semen is collected once a week using.an artificial.vaginal method from a three-year-old Angus bull in an undisturbed or healthy body and reproductive organs. Semen was diluted with T-EY diluent which had been added with zinc at several levels, 0 mg control treatment (T0), 10.mg (T1), 20.mg (T2), 30.mg (T3), 40 mg (T4). The diluted semen was.stored at a temperature of 3-5oC, then observed every 24 hours for.motility, viability,.abnormalities and survival of.spermatozoa to a minimum motility of 40%. The.results showed.that the addition of 20 mg of zinc in the T-EY.diluent (T2) resulted in a higher.quality of liquid semen of Angus bull (P˂0.05) than other.treatments. The quality of the liquid semen (motility, viability, abnormalities and survival of spermatozoa) were motility 44±5.75%, viability 48.39±13.59%, abnormality 6.91±1.85% and durability.live 5.60±0.89 days. It was.concluded that the.addition.of 20 mg zinc in the.tris-egg yolk diluent produced liquid semen of Angus bull with higher quality than other treatments.
Pengaruh Kombinasi Pengencer Susu Kacang Kedelai Sangrai dan Sitrat Modifikasi terhadap Kualitas Spermatozoa Babi Landrace Loka, Kristina; Nalley, W. Marlene; Kune, Petrus
Animal Agricultura Vol 2 No 1 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i1.59

Abstract

The purpose of this study was to evaluate the effect of the combination of roasted soy milk (RSM) diluent and and modified citrate (C) diluent on the quality of landrace boar spermatozoa. The material used in this study was fresh semen of 2-year-old male landrace pigs. This study used a complete randomized design method consisting of 5 treatments and 5 repeats. The treatment was, T0: roasted soybean milk (RSM) 100% + citrate (C) 0%, T1: RSM 75% + C 25%, T2: RSM 50% + C 50%, T3: RSM 25% + C 75% and T4: RSM 0% + C 100%. Diluted semen was stored at 18-20°C and evaluated every 8 hours. The parameters studied are motility, viability, abnormalities and survival of spermatozoa. The data obtained were analyzed using analysis of variance and followed by Duncan’s test. The results showed that T3 significantly differed (P<0,05) on motility, viability and liveability, but not significantly different (P>0,05) on spermatozoa abnormalities. The quality of spermatozoa in T3 treatment was 45.80±1.30% motility, 54.27±4.71% viability, 6.26±0.47% abnormality and 40.00±0.00 hours survival. It can be concluded that the combination of roasted soy milk diluent (RSM) 25% + citrate (C) 75% was an effective treatment in maintaining the quality of landrace boars spermatozoa for 40 hours of storage.
Pengaruh Penambahan Ekstrak Biji Kelor Kering (Moringa oleifera Lam) dalam Pengencer Beltsville Thawing Solution terhadap Kualitas Semen Cair Babi Landrace Pandahuki, Fransiska Onya; Nalley, W. Marlene; Uly, Kirenius; Mata Hine, Thomas
Animal Agricultura Vol 2 No 1 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i1.67

Abstract

The aim of this research was to determine the effect of adding dry moringa seed extract (DMSE) added to the Beltsville thawing solution (BTS) diluent on the quality of landrace boar semen during storage. This research used experimental methods and a completely randomized design  consisting of 5 treatments and 5 replications to form 25 experimental units. The treatment in question was : T0 = BTS EY + 0% DMSE, T1 = BTS EY + 1% DMSE, T2 = BTS EY + 2% DMSE, T3 = BTS EY + 3% DMSE, T4 = BTS EY + 4% DMSE. This diluted semen will be stored at a temperature of 18-20ºC and evaluation of motility, viability, abnormalities and survival of spermatozoa was carried out every 8 hours of storage. Data was analysis of the average, standard deviation, variance and continued with the Duncan test. The results showed that T2 treatment with a 2% DMSE level at 32 hours of storage gave the best results (P<0.05) compared to other treatments, with motility values (51, 80±4.71), viability (65.54±5.00), abnormality (5.81±0.81 and survival value of (38.89±1.21). It was concluded that the addition of EBKK at a level of 2% to the BTS diluent provided a good response in maintaining motility, viability and abnormalities.
Co-Authors Aloysius Marawali Ina Mato, Sefania Loka, Kristina Pandahuki, Fransiska Onya Petrus Kune Siena, Sofia M.S. Thomas Mata Hine Uly, Kirenius