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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Peternakan Indonesia Jurnal Ternak Tropika Livestock and Animal Research JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Peternakan Nusantara Jurnal Ilmu Peternakan Terapan Wahana Peternakan Jurnal Nukleus Peternakan COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Jurnal Peternakan Lahan Kering Jurnal Sosial dan Sains Jurnal Kedokteran Hewan Lentera: Multidisciplinary Studies Journal of Livestock and Animal Health (JLAH) Animal Agricultura Journal of Innovative and Creativity Lentera: Multidisciplinary Studies
Thomas Mata Hine
Fakultas Peternakan, Kelautan Dan Perikanan, Universitas Nusa Cendana
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CHARAKTERISTICS OF BALI CATTLE YOUNG BULL UNDER TRADITIONALLY LIVESTOCK SYSTEM IN MERBAUN VILLAGE REGENCY OF KUPANG Baharun, Abdullah; L. L Belli, Henderiana; Hine, Thomas M
Jurnal Peternakan Nusantara Vol 3, No 1 (2017)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.065 KB) | DOI: 10.30997/jpnu.v3i1.850

Abstract

An experiment had been conducted in Merbaun Village, Regency of Kupang for three months. The objective of the experiment was to know characteristics of Bali cattle Bull i.e. age and body weight, body score condition, linier body size (e.g. chest girth, body length, and shoulder height), libido, scrotum circumference, and semen production. 125 of Bali cattle Bull with aged about 2-2,5 years were used  in physical measured while nine Bull were choosen randomly to measure libido and semen production. The results of the experiment showed that the average age and body weight were 25.83 ± 0.3719 months and 207.37 ± 1.84 kg, with BCS of 3.97 ± 0.04  (from 5 score), and the chest girth, body length, and shoulder height were 158.27 ± 0.71 cm, 106.87 ± 0.26 cm, and 108.55 ± 0.37 cm, respectively. About 77 head (61.6%) was brown colour, and the other 48 (38.4%) was black, with moderate of capacity of libido (score 7.22 ± 0.66  from  9 score). The average of scrotum circumference was 25.67±1.20 cm, volume of semen were  2.54±0.69 ml and sperm concentration was 1.26 x 109 per ml repectively. Of the total of 125 Bali cattle young bull, only 26% fulfilled the Indonesia Standard of Bali young bull.Key word : young bull, bali cattel, traditionally livestock.
CHARAKTERISTICS OF BALI CATTLE YOUNG BULL UNDER TRADITIONALLY LIVESTOCK SYSTEM IN MERBAUN VILLAGE REGENCY OF KUPANG Baharun, Abdullah; L. L Belli, Henderiana; Hine, Thomas M
Jurnal Peternakan Nusantara Vol. 3 No. 1 (2017)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.065 KB) | DOI: 10.30997/jpnu.v3i1.850

Abstract

An experiment had been conducted in Merbaun Village, Regency of Kupang for three months. The objective of the experiment was to know characteristics of Bali cattle Bull i.e. age and body weight, body score condition, linier body size (e.g. chest girth, body length, and shoulder height), libido, scrotum circumference, and semen production. 125 of Bali cattle Bull with aged about 2-2,5 years were used  in physical measured while nine Bull were choosen randomly to measure libido and semen production. The results of the experiment showed that the average age and body weight were 25.83 ± 0.3719 months and 207.37 ± 1.84 kg, with BCS of 3.97 ± 0.04  (from 5 score), and the chest girth, body length, and shoulder height were 158.27 ± 0.71 cm, 106.87 ± 0.26 cm, and 108.55 ± 0.37 cm, respectively. About 77 head (61.6%) was brown colour, and the other 48 (38.4%) was black, with moderate of capacity of libido (score 7.22 ± 0.66  from  9 score). The average of scrotum circumference was 25.67±1.20 cm, volume of semen were  2.54±0.69 ml and sperm concentration was 1.26 x 109 per ml repectively. Of the total of 125 Bali cattle young bull, only 26% fulfilled the Indonesia Standard of Bali young bull.Key word : young bull, bali cattel, traditionally livestock.
Tampilan Estrus dan Angka Kebuntingan Sapi Bali Pascapemberian Ekstrak Pituitari Wilmientje Marlene Nalley; Petrus Kune; Thomas Mata Hine
Acta VETERINARIA Indonesiana Vol. 5 No. 2 (2017): Juli 2017
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.818 KB) | DOI: 10.29244/avi.5.2.74-80

Abstract

Tujuan penelitian ini adalah untuk menguji potensi ekstrak pituitari sebagai sumber hormon alami yang murah dan aplikatif untuk meningkatkan produktivitas induk sapi bali. Kegiatan penelitian diawali dengan koleksi kelenjar pituitari dari rumah potong hewan dan diawetkan menggunakan aseton konsentrasi dengan bertingkat. Kelenjar pituitari kemudian dilarutkan dengan aquabidest menggunakan mortar  hingga  larut, larutan kelenjar pituitari disentrifugasi, dan supernatan disimpan sebagai ekstrak pituitari (EP). Dua belas ekor sapi bali dibagi ke dalam 4 kelompok. Kelompok I dosis 0 ml (P0, kontrol), kelompok II 10 mg/kg berat badan (BB) (P1), Kelompok III 20 mg/kg BB (P2), dan kelompok IV 30 mg/kg BB (P3).  Variabel penelitian adalah tampilan estrus, tingkat ovulasi, angka kebuntingan dan service per conception. Hasil penelitian menunjukkan pemberian  20 mg/kg BB EP pada induk sapi bali mampu memperpendek siklus estrus (P<0,05) yaitu 11,67 hari dibandingkan dengan kontrol 27 hari; meningkatkan angka kebuntingan hingga 100% vs 0%, dan menurunkan service per conception yaitu 1,00 vs 3,33. Tidak terdapat perbedaan dalam ovulatiton rate dalam  intensitas estrus antar perlakuan (P>0,05). Kesimpulan penelitian ini adalah  esktrak pituitari dapat meningkatkan produktivitas induksi sapi bali, dengan dosis terbaik yaitu 20 mg/kg BB. 
Efek Ekstrak Hipofisa Sapi terhadap Pertambahan Bobot dan Umur Pubertas Mencit Betina (Mus musculus) A. Kaka; W. M. Nalley; T. M. Hine
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 20, No 2 (2018): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.20.2.91-98.2018

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak hipofisa sapi terhadap pertambahan bobot badan dan umur pubertas mencit betina. Materi penelitian adalah mencit betina strain swiss webster yang berumur 21 hari, sebanyak 48 ekor dengan kisaran berat badan (BB) 11-13 gram (±12 gram). Rancangan percobaan adalah rancangan acak lengkap dengan 4 perlakuan dan 12 ulangan yaitu penyuntikan ekstrak hipofisis dengan dosis 0 mg/g BB (P0, kontrol); 0,05 mg/g BB (P1); 0,10 mg/g BB (P2); 0,15 mg/g BB (P3). Penyuntikan dilakukan dua kali seminggu sejak umur lepas sapih hingga pubertas, secara intraperitoneal. Data pertambahan bobot badan dan umur pubertas dianalis dengan ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa perlakuan ekstrak hipofisa berpengaruh nyata (P<0,05) terhadap PBB dan umur pubertas mencit betina. Dari hasil penelitian dapat disimpulkan bahwa penyuntikan esktrak hipofisa sapi dapat meningkatkan pertambahan bobot badan dan mempercepat umur pubertas mencit betina, dengan dosis ekstrak hipofisa terbaik adalah 0,15 mg/g bobot badan.
Efektivitas Air Buah Lontar dalam Mempertahankan Motilitas, Viabilitas dan Daya Tahan Hidup Spermatozoa Sapi Bali Thomas MataHine; Burhanuddin .; Aloysius Marawali
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.549 KB)

Abstract

The objective of this study was to determine the effectiveness of palmyra juice in maintaining motility,viability, and longevity of bali cattle sperm during in vitro storage. Semen collected from a four year oldbali bull had been trained to serve an artificial vagina. Good quality semen divided into four tubes to betreated with: without extender (negative control), citrate-egg yolk (citrate-EY) and coconut water-egg yolk(CW-EY) as positive control; and palmyra juice-egg yolk (PJ-EY), and then stored in a refrigerator attemperature 3 - 8oC. Each treatment was repeated five times to form 20 experimental units. The resultsshowed that up to the fourth day of storage, sperm preserved in PJ-EY had motility 44%, higher (P<0.05)than citrate-EY, CW-EY, and without extender, i.e 30%, 18%, and 0%, respectively. PJ-EY extender alsoyield sperm viability 60%, higher (P<0.05) than citrate-EY, CW-EY, and without extender i.e 50%, 32% and0%, respectively. Sperm livability achieve 11.20 days in PJ-EY, longer (P <0.05) than citrate-EY, CW-EYand without extender, i.e 9.60 days, 7.60 days , and 1.31 days, respectively. In conclusion that palmyrajuice-egg yolk extender effective to maintain motility, viability, and longevity of bali cattle sperm duringin vitro preservation.
PRODUCTION OF EMBBRYONIC STEM CELLS FROM INNER CELL MASS OF BLASTOCYST ISOLATED BY ENZYMATIC AND IMMUNOSURGERY METHODS Thomas Mata Hine; Iman Supriatna; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1169.128 KB)

Abstract

The objective of the research is determining the ICM isolation method to produce ESC. Blastocyst stage of DDy mice embryos were used in this study. Zona pellucida of blastocysts were removed by 0.25% pronase, the ICM isolation were done by enzimatic or immunosurgery method, and then they were cultured in DMEM-high glucose supplemented with mercaptoethanol, gentamycin, fetal bovine serum, and cumulus cells as feeder layer. The result of the research indicated that immunosurgery method yielding attachment rate and number ESC colony 93.85% and 43.08%, respectively, higher (P<0.05) than enzimatic method that weree 79.63% and 18.52%, respectively, but the viability of ICM cells were equal (P >0.05) that are 93.59% in enzymatic method and 98.56% in immunosurgery method. This research concluded that immunosurgery more effective method for isolation of ICM and ESC production than enzymatic method.
Penyuntikan Tiga Kali Ekstrak Pituitari Selama Induk Sapi Bali Bunting Meningkatkan Bobot Lahir dan Produksi Air Susu Wilmientje Marlene Nalley; Thomas Mata Hine; Petrus Kune
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.27 KB) | DOI: 10.19087/jveteriner.2021.22.2.271

Abstract

Penelitian ini bertujuan untuk menguji efek pemberian ekstrak pituitari (EP) terhadap produktivitas induk sapi bali bunting. Sebanyak 12 ekor sapi bali bunting 2,5 bulan dibagi ke dalam empat perlakuan yaitu: tanpa injeksi EP (P0, kontrol), injeksi EP satu kali pada umur kebuntingan 2,5 bulan (P1), injeksi EP dua kali pada umur kebuntingan 2,5 dan 5,0 bulan (P2), dan injeksi EP tiga kali pada umur kebuntingan 2,5, 5,0 dan 7,5 bulan (P3). Dosis EP yang diberikan adalah 20 mg/kg bobot badan. Parameter yang diamati adalah jumlah dan morfologi corpus luteum (CL), bobot lahir anak, dan produksi susu induk. Data yang diperoleh dianalisis dengan uji sidik ragam dan dilanjutkan dengan Uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa injeksi EP tidak memengaruhi (P>0,05) jumlah dan morfologi CL. Bobot lahir anak (19,87 kg) dan produksi susu induk harian (1.707,18 mL) tertinggi (P<0,05) dihasilkan oleh sapi bali yang diinjeksi EP tiga kali. Penelitian ini menyimpulkan bahwa penyuntikan EP tiga kali efektif meningkatkan bobot lahir anak dan produksi susu induk sapi bali.
Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION) Thomas Mata Hine; Kirenius Uly; Wilmientje Marlene Nalley; Heri Armadianto
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.838 KB) | DOI: 10.19087/jveteriner.2019.20.1.93

Abstract

Dimethyl Sulfoxide (DMSO) is one type of cryoprotectant which has a low molecular weight so that it is easier to enter cells when cryopreservation. The purpose of this study was to explore the optimal concentration of DMSO in modified coconut water (mCW) extender that were able to maintain frozen sperm quality of bali bulls. Semen was collected from two four-year old bali bulls by artificial vagina. Good quality semen diluted with mCW (young coconut water + 20% egg yolk + 7.5 % moringa leaf extract) and supplemented by 3, 5, or 7% DMSO. Semen was filled into 0.25 ml ministraw, and was incubated in a refrigerator at 5°C for four hours, frozen on the surface of liquid nitrogen for 10 minutes and then dipped into liquid nitrogen. The quality of post thawing sperm was measured 24 hours later by placing the ministraw of frozen semen into water at 37oC for 30 seconds. Data were analyzed by analysis of variance and continued with Duncan test. Postthawing observations showed that bali bulls sperm cryopreserved at 3% DMSO yielded higher motility and viability (p<0.05) i.e. 36 and 44.15%, than DMSO 5% i.e. 18 and 23.65%, and DMSO 7% i.e. 7 and 12.62%. The recovery rate of sperm cryopreserved at 3% DMSO was also higher (p<0.05) than DMSO 5 and 7%, successively 45.65, 23.06, and 8.86%. The results of this study concluded that the optimal concentration of DMSO in mCW diluent to maintain frozen sperm quality of bali bulls was 3%.
Sel Kumulus Sebagai Feeder Layer pada Kultur Stem Cells Embrionic Mencit (CUMULUS CELLS AS FEEDER LAYER IN CULTURE OF MOUSE EMBRYONIC STEM CELLS) Thomas Mata Hine; Arief Boediono; Iman Supriatna; Dondin Sajuthi
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.795 KB)

Abstract

The purpose of this study was to verify the effectiveness of cumulus cells as a feeder layer in supportingthe growth of mouse embryonic stem cells. Embryos at blastocyst stage were exposed in pronase solution,and then incubated in rabbit anti-mouse antibody and guinea pig complement to lyse and separate thetrophoblast cells from the inner cell mass. Inner cell mass subsequently cultured in a petri dish containinga cumulus feeder layer, mouse embryonic fibroblasts, or without a feeder layer, in stem cells medium. Theresulting stem cells colony passaged in trypsin solution, pipetted repeatedly to produce subcolonies orsingle cells, and cultured as before in some new petri dishes. Characterization of stem cells was identifiedby using alkaline phosphatase staining. The results showed the effectiveness of cumulus cells as feederlayer for culture of mouse embryonic stem cells was comparable with mouse embryonic fibroblasts, andboth of them were better than without a feeder layer method. The number of primary colony, cell lines, andcolony growth rate increased 41.30, 8.70, and 54.20%, respectively, while doubling time was shorter 10:21hours compared to the growth without feeder layer method. These results prove that the cumulus feederlayer effectively supports the growth of mouse embryonic stem cells.
Aktivasi dan Tingkat Perkembangan Embrio Partenogenetik Mencit Setelah Dipapar Calcimycin dan Ionomicyn (ACTIVATION AND DEVELOPMENT RATE OF MICE PARTHENOGENETIC EMBRYOS EXPOSURED IN CALCIMYCIN AND IONOMICYN) Wilmientje Marlene Nalley; Thomas Mata Hine
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.332 KB)

Abstract

The aim of study was to find out the best concentration and exposure time of calcimycin andionomycin in order to produce parthenogenetic embryos. Female Swiss Webster mice were fisrtlyprimed with Pregnant Mare’s Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (hCG)with an interval of 48 hours. Sixteen hours after injection of hCG oocyte was collected by Dulbecco’sPhosphate Buffer Saline (dPBS) as a flushing medium. To separate the eggs from cumulus cells wereused hyaluronidase enzyme. The good quality oocytes were incubated in activation medium that isionomycin or calcimycin with a concentration of 3, 6, or 9 ìM and exposure time 1, 4, or 7 minutes. Toyield diploid embryos were used 5 ?g/ml cytochlasin B for four hours at 37°C, 5% CO2. Activatedoocytes characterized by the formation of pronuclei washed three times in Potassium SimplexOptimization Medium (KSOM) and subsequently cultured in the same medium until blastocyst stage.The results showed that oocytes activated at calcimycin, the best results was presented at concentration6 ?M and exposure time four minutes, i.e. activation rate reached 96%, cleavage rate 82% and blastocystrate 28%. On the other hand, oocytes activated in ionomycin, the best results was presented atconcentration 3 ?M and exposure time four minutes, i.e. activation rate reached 82%, cleavage rate64% and blastocyst rate of 4%. It was concluded that the best concentration and exposure timecalcimycin on mice oocytes were 6 ?M for four minutes, whereas ionomycin were 3 ?M for four minutes.
Co-Authors A. Kaka A. Parakkasi Abdullah Baharun Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ayang C. Tafuli Bayu Rosadi Bette, Yustiany Y Bire Lawa, Alvrado Burhanuddin * Burhanuddin . D. J. Djawapatty Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Franky M S Telupere Fransiska Onya Pandahuki George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. kirenius uly Kirenius Uly Kirenius Uly Kirenius Uly Kirenius Uly Kristina Meldiana Hayati L. L Belli, Henderiana L. L Belli, Henderiana Lawa, Alvrado B Mardan Laes Papituan Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu, Agustinus R. Sefania Ina Mato Selsiana Dongkot Setyani, Ni M P Siena, Sofia M.S. Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly, Kirenius W. Marlene Nalley W. Marlene Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanes Paulus Martinus Bay Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yustiany Yuliani Bette Yusuf Sae