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All Journal Research Journal of Life Science JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGRIC
Lestiyani, Ayu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Medicine & Pharmacology Social Sciences

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Potential Analysis of Lemna sp. Extract as Immunostimulant to Increase Non-Specific Immune Response of Tilapia (Oreochromis niloticus) against Aeromonas hydrophila Armando, Eric; Lestiyani, Ayu; Islamy, R. Adharyan
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.6

Abstract

Lemna sp. is known to have several bioactive compounds and polysaccharide macromolecules that can function as immunomodulators to affect non-specific immune responses to increase the body's resistance to pathogens. This study aims to determine the potential of catfish eye extract as an immunostimulant by observing non-specific tilapia immune parameters. The extraction method used was 96% ethanol maceration for 2 days with a ratio of 1: 4. The experimental design used a Completely Randomized Design with 5 treatments (doses 0.2, 0.4, 0.6 mg/kg, control + and control -) and 3 replications. The non-specific parameters of immunity observed included total plasma protein (Bradford method), superoxide dismutase and lysozyme activity. The data obtained will be analyzed using ANOVA, if there is a significant difference, it will be further tested with Duncan Multiple. Range Test (DMRT). The results showed that the highest total plasma protein was found in treatment C (giving an extract of 0.3 mg/kg body weight) with an average total plasma protein after 12 days of maintenance of 4.99 g / dL. The extract dose of 0.3 mg/body weight showed a rapid decrease in SOD and increase Lysozyme activity.
Pathogenicity and Detection of Phytohormone (Gibberellic Acid and Indole Acetic Acid) Produced by Fusarium spp. that Causes Twisted Disease in Shallot Lestiyani, Ayu; Wibowo, Arif; Subandiyah, Siti
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 5 No. 1 (2021): June 2021
Publisher : Department of Plant Protection, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.1.24-33.2021

Abstract

The twisted disease is one of the essential diseases in shallots caused by Fusarium spp. This study aimed to study pathogenicity and identify Fusarium species isolated from shallot plants with twisted symptoms in Nganjuk and Bantul areas. The Fusarium isolates were identified and then tested for pathogenicity levels and the effect of the hormones GA3 and IAA on shallot symptoms. Molecular identification using NF2 and NF4 successfully identified one isolate of Fusarium oxysporum, three isolates of F. acutatum, and three isolates of F. solani. Each of these species produces different symptoms. Pathogenicity test showed that all isolates had disease incidence reaching 100%, except isolates of F. solani1 causing wilt and F. solani3 causing twisted have the lower disease incidence were 77.8% and 77.7%, respectively. The investigation caused twisted shallot related to different symptoms was tested using the Thin Layer Chromatography (TLC) method. The result indicates that all isolates did not find IAA hormone. In contrast, the hormone GA3 was found in F. solani2 and F. solani3 isolates, caused bulb rot and twisted disease, respectively. Detection of IAA, GA3, and other hormones in shallot plants showed different symptoms should be studied further.
INVESTIGATING THE EFFECTS OF BAMBOO BIOCHAR ON FERTILE SOIL FOR IMPROVING THE PRODUCTION OF Brassica oleraceae Lestiyani, Ayu; Purnomo, Erry; Widyaningsih, Septy; Fadillah, Khrisna
Agric Vol. 35 No. 2 (2023)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2023.v35.i2.p169-180

Abstract

Cabbage (Brassica oleracea var capitate), broccoli (B. oleraceae L. var italica), carrots, and cauliflower (B. oleracea var botrytis) are the primary vegetable crops grown in Ngablak. The role of chemical fertilizers in crop enhancement cannot be avoided in the production of vegetables. Chemical fertilizers come in a variety of forms, including nitrogenous, phosphate, and potassium fertilizers. Fertilizer use not only boosts crop yield, but also modifies the physical, chemical, and biological aspects of the soil. However, the continual use of chemical fertilizers has led to a loss in soil organic matter (SOM) and a deterioration in the quality of agricultural soil. The excessive use of chemical fertilizers hardens the soil, diminishes soil fertility, pollutes the air, water, and soil, and depletes vital soil nutrients and minerals, consequently posing environmental risks. This research breakthrough is needed to reduce chemical fertilizers. Biochar, a pyrolyzed or anaerobically-grown biomass, could be used. This study examined the effects of biochar on disease suppression and yield in Brassica oleracea plants in rich soils in Magelang, Central Java. The treatment of this study was control, biochar, biochar+compost. The results of this study can be concluded that the maximum wet and dry weight of broccoli and cabbage plants was achieved in the biochar+compost treatment. Biochar and biochar+compost can enhance the nutrient content of the soil in broccoli, cauliflower, and cabbage. In addition, biochar has an effect (positive or negative influence) on disease in test plants. During June observations, the controls had the highest incidence and plant disease severity, whereas adding biochar and biochar+compost reduced disease incidence and plant disease severity. Increased potassium in conjunction with changes in the chemical characteristics i.e. increasing pH, total N, and C- organic of the soil results in the formation of resistance.
Co-Authors Arif Wibowo Armando, Eric Erry Purnomo Fadillah, Khrisna Islamy, R. Adharyan Siti Subandiyah Widyaningsih, Septy