Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

1.879

P-Index

This Author published in this journals

All Journal Jurnal Fitopatologi Indonesia Vegetalika Jurnal Ilmu Pertanian BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) jurnal Ilmu Pertanian (Agricultural Science)

Siti Subandiyah

Cultivation Of Agriculture Department, Faculity Of Agriculture, Gadjah Mada University

Author-ID : 2898475

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

Published : 20 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Deteksi Virus Terbawa Umbi Benih pada Bawang Merah Kultivar Biru Bantul Nurviani Nurviani; Sri Sulandari; Susamto Somowiyarjo; Siti Subandiyah
Jurnal Fitopatologi Indonesia Vol 12 No 5 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Virus infection on shallots may cause mosaic of leaves and plant dwarf. It is very important to know the identity of the virus as a requirement in determining viral disease management strategy. The research is subjected to identify seed- transmitted viruses from shallot bulbs. Detection of virus from bulb was conducted by growing on test, followed by mechanical inoculation on shallot cultivar Biru Bantul, Chenopodium amaranticolor, and Nicotiana tabacum and morphological observation of virus particle by electron microscope. About 2 weeks after inoculation symptoms was observed, i.e. mosaic and dwarf on shallot cultivar Biru Bantul, local necrotic on C. amaranticolor, but symptomless on N. tabacum. Filamentous particles of 650 nm and 800 nm in length was observed under electron microscope from plants with mosaic and dwarfing symptom, respectively. This indicated the presence of 2 different seed-transmitted viruses on infected shallots. Two species of shallot viruses, i.e. Shallot latent carlavirus (SLV) and Onion yellow dwarf potyvirus (OYDV) was most likely the main seedborne viruses on shallot bulb. Identification based on nucleic acid and protein sequence analysis is necessary for further confirmation

Elimination of shallot bulb viruses through heat treatment Margo Sulistio; Endang Sulistyaningsih; Siti Subandiyah
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Shallot (Allium cepa L. Aggregatum group) is usually cultivated vegetatively. As a result, viruses tend to accumulate within the host plants and spread to healthy plants every crop cycle, reducing yield and bulb quality. There are a very limited number of studies about the elimination of shallot viruses through heat treatment. The objective of this research was to eliminate shallot viruses through heat treatment to produce virus-free plantlets. The leaves of Biru Lancor with specifc visual virus symptoms were detected by Reverse Transcription–Polymerase Chain Reaction (RT-PCR). Then bulbs of Biru Lancor that were positively infected by viruses were used as materials for heat treatment. The treatments were a control (without treatment), electric treatment at 15 mA for 10 minutes, heat treatment in an incubator at 37°C for 4 weeks, heat treatment in a waterbath at 45°C for 60 minutes, and combination of heat treatment in an incubator at 37°C for 4 weeks and heat treatment in a waterbath at 45°C for 60 minutes. After being subjected to heat treatment, the pseudo stem were cultivated in the MS Medium + 1 mg/L BAP + 1 mg/L IBA.Virus detection by RT-PCR was conducted 28 days after planting using samples of leaves from each plantlet. The results of this research showed that the treatments of electric treatment at 15 mA for 10 minutes and combination of heat treatment in the incubator at 37°C for 4 weeks and heat treatment in the waterbath at 45°C for 60 minutes could suppress the incidence of Shallot latent virus (SLV) until 100%. Heat treatment might have an important role in the degradation of virus particles by boosting Virus-Induced Gene Silencing (VIGS) as plant responses to virus infection.

Pengaruh Agensia Bermanfaat dan Paket Pemupukan Magnesium, Boron dan Silikon terhadap Hasil dan Kualitas Buah Pisang Mas (Musa acuminata L.) Ramot Christian; Eka Tarwaca Susila Putra; Siti Subandiyah
Vegetalika Vol 5, No 4 (2016)
Publisher : Universitas Gadjah Mada

Penelitian bertujuan untuk 1) menentukan ada atau tidaknya pengaruh interaksi aplikasi agensia bermanfaat dengan paket pemupukan Mg, B, dan Si terhadap hasil dan kualitas buah pisang mas, dan 2) menentukan kombinasi terbaik antara faktor agensia bermanfaat dan paket pemupukan Mg, B, dan Si yang mampu memaksimalkan hasil dan kualitas buah pisang mas. Penelitian di lapangan dilaksanakan pada Mei 2015 – April 2016, dengan menggunakan Rancangan Petak Terbagi (RPT) dua faktor dengan tiga blok sebagai ulangan. Faktor pertama berupa aplikasi agensia bermanfaat dijadikan sebagai petak utama, sementara itu faktor kedua berupa aplikasi pemupukan Mg, B, dan Si dijadikan sebagai anak petak.Faktor agensia bermanfaat terdiri atas empat taraf yakni 1) Trichoderma sp., 2) Trichoderma sp. + mikoriza, 3) Trichoderma sp. + molass, 4) Trichoderma sp. + mikoriza + molass, 5) Tanpa aplikasi agensia bermanfaat. Kemudian faktor aplikasi paket pemupukan Mg, B, dan Si terdiri atas dua taraf yakni 1) 60 g/tanaman Mg + 2,12 g/tanaman B + 0,92 g/tanaman Si, 2) Tanpa aplikasi paket pemupukan Mg, B, dan Si. Variabel utama yang diamati meliputi karakter fisiologis, karakter anatomi, dan komponen hasil pada buah pisang mas. Data yang diperoleh dianalisis varian (ANOVA) pada taraf 5% dan berikutnya diuji lanjut dengan uji beda nyata terkecil (BNT) apabila terdapat beda signifikan antar perlakuan. Hasil penelitian memberikan informasi bahwa terdapat pengaruh interaksi antara faktor agensia bermanfaat dengan paket pupuk Mg, B, dan Si pada variabel kandungan pektin, lignin, selulosa, ketebalan dinding sel, diameter floem, dan diameter xilem kulit buah pisang mas. Kemudian kuantitas hasil dan komponen hasil pisang mas tidak dipengaruhi oleh interaksi antara faktor agensia bermanfaat dengan paket pupuk Mg, B, dan Si, maupun pengaruh individual dari kedua faktor tersebut. Sementara itu kualitas hasil pisang mas yang diindikasikan oleh variabel kekerasan kulit jari buah menjadi lebih baik akibat perlakuan paket pupuk Mg, B, dan Si, namun tidak dipengaruhi oleh macam agensia bermanfaat.

Fingerprint Analysis of DNA Fragments of Pseudomonas solanucearum E.F. Smith Isolates Siti Subandiyah; A. C. Hayward; M. Fegan
Jurnal Ilmu Pertanian Vol 4, No 7 (1991): September
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.58677

The Use Of Pulse Field GelElectrophoresis For Megaplasmid Isolatioan Of Pseudomonas solanacearum Siti Subandiyah; Triwibowo Yuwono; Wayan T. Artama
Jurnal Ilmu Pertanian Vol 5, No 4 (1994): September
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.60011

Karakterisasi dan Deteksi Cepat Bakteri Penyebab Penyakit Darah pada Pisang Nur Edy; Siti Subandiyah; Christanti Sumardiyono; Jaka Widada
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Blood disease of banana is one of the most serious banana disease in Indonesia. Although the disease has became the subject of quarantine it eventually spread and found in most provinces in Indonesia. The aim of this research were to identify the blood disease bacterium (BDB) using morphological observation, biochemical assay, pathogenicity testing of hosts range using infectivity titration and rapid detection by Polymerase Chain Reaction (PCR). The results showed that the blood disease bacterium could be differentiated from Ralstonia solanacearum race 2, the causal agent of Moko disease and R. solanacearum tobacco isolates. BDB isolates were not able to hydrolyze gelatin, Tween 80, starch, and were not able to produce nitrite from nitrate. They were only able to produce acid from galactose and glycerol. The pathogenicity test indicated that the BDB was only able to infect the banana/plantain and was not able to infect tomato, eggplant, and chili. Rapid detection using PCR method showed that the 121F/R primers was able to amplify the BDB genome and was not able to amplify the genome of R. solanacearum tobacco isolates.

Hubungan Antara Aktivitas Poligalakturonase dengan Virulensi RAS 4 Fusarium oxysporum f.sp. cubense Arif Wibowo; Siti Subandiyah; Christanti Sumardiyono; Liliek Sulistyowati; Peter Taylor
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11871

One of the major constraints of banana plantation in Indonesia is the occurrence of fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense. The pathogen produced series of cell wall degradation extracellular enzymes which have important roles in pathogenicity. Many studies have been conducted to know the role of degrading enzyme banana pectin is the major component of cell wall. Many pectinolytic enzymes such as polygalacturonase and others have been isolated from many fungal plant pathogens. The study was aimed to know the role of polygalacturonase towards the virulence of race 4 of F. oxysporum f.sp. cubense. The result showed that from 10 isolates of race 4 of F. oxysporum f.sp. cubense, the most virulent isolate was Lmp1 followed by Srg1, Bgl6, Mln1, Bgl3, A13, Bnt2, Gnk3, Kjg1 dan Wsb5. This was indicated by high and low percentage of wilting leaves of banana cultivar Cavendish when they were inoculated with these isolates. Incubation period varied from 3 to 6 weeks after inoculation SDS-PAGE showed that polygalacturonase, mostly PG1 and PG2, was secreted by these isolates, whereas PG3 was only found in growing cultures of Gnk3 and Wsb5 isolates. Detection of polygalacturonase activity with diffusion agar and reducing sugar methods showed that the activity of polygalacturonase secreted by F. oxysporum f.sp. cubense in the growing culture had no correlation with the virulence of the fungal pathogen.

Deteksi Penyakit Bacterial Fruit Blotch pada Melon Menggunakan ELISA Utik Windari; Tri Joko; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 19, No 1 (2015)
Publisher : Universitas Gadjah Mada

Bacterial fruit blotch (BFB) caused by Acidovorax citrulli is a serious seedborne disease in Cucurbitaceae causing 90-100% yield losses. The aim of this study was to explore BFB symptom on melon and also to detect A. citrulli infection in commercial seed and symptomatic fruits from the field in Yogyakarta Special Region province and its surrounding using DAS-ELISA method. Samples include melon from Sleman, Bantul, Kulon Progo, Gunung Kidul, Magelang, Purworejo regencies while commercial seeds i.e. Action 434, Glamour and Mai 116 were collected. DASELISA detection method used reagent set from Agdia. Based on the field observation, this study found melon commercial fruit shares similar symptom with BFB, which showed discrete oily dark green spots, while the netting failed to develop over necrotic areas, resulting in smooth sunken spots. DAS-ELISA detection revealed that samples collected from Jetak village, district of Mungkid, Magelang and from Bligo village, district of Ngluwar, Magelang and in commercial seed Mai 116 were positively infected by A. citrulli.INTISARIBacterial fruit blotch (BFB) merupakan penyakit penting pada famili Cucurbitaceae yang disebabkan oleh Acidovorax citrulli. Penyakit ini dilaporkan dapat menurunkan hasil mencapai 90-100%. Tujuan dari penelitian ini adalah untuk mengetahui gejala penyakit BFB pada melon dan deteksiA. citrulli pada benih komersial dan sampel buah bergejala dengan metode DAS-ELISA di DIY dan sekitarnya. Pengambilan sampel dilakukan di kabupaten Sleman, Bantul, Kulon Progo, Gunung Kidul, Purworejo dan Magelang. Selain dari lapangan, diuji pula benih melon komersial yaitu Action-434, Glamour dan Mai 116. Metode deteksi dengan ELISA menggunakanreagent set dari Agdia. Dari hasil pengamatan di lapangan ditemukan buah melon dengan gejala yang mirip dengan gejala BFB yaitu adanya becak berwarna hijau tua kebasahan pada permukaan buah, jaring tidak terbentuk sempurna dan pada bagian daging buah di bawah becak tadi membusuk. Hasil deteksi dengan DAS-ELISA mengindikasikan bahwa A. citrulli terdeteksi pada sampel yang berasal dari desa Bligo, kecamatan Ngluwar dan desa Jetak, kecamatan Mungkid, kabupaten Magelang, serta pada benih komersial MAI 116.

Potensi Bakteri Endofit Asal Tanaman Pisang Klutuk (Musa balbisiana Colla) Sebagai Pendukung Pertumbuhan Tanaman Triastuti Rahayu; Yekti Asih Purwestri; Siti Subandiyah; Donny Widianto
Al-Kauniyah: Jurnal Biologi Vol 14, No 2 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i2.19140

AbstrakBakteri endofit yang terdapat di tanaman pisang Klutuk dan keterkaitannya dengan sifat ketahanan tanaman pisang Klutuk pada cekaman biotik dan abiotik belum dilaporkan dalam publikasi ilmiah. Sebanyak 93 isolat bakteri endofit telah diperoleh dari pisang Klutuk, tetapi belum diketahui kemampuannya sebagai pendukung pertumbuhan tanaman (PPT). Penelitian ini bertujuan untuk mengetahui karakter isolat-isolat bakteri endofit dari pisang Klutuk sebagai pendukung pertumbuhan tanaman. Kelompok bakteri Gram positif dan negatif ditentukan dengan metode pewarnaan Gram. Kemampuan memfiksasi nitrogen (N2), memproduksi asam indol asetat (AIA), dan antagonisme terhadap Fusarium oxysporum f. sp. cubense (Foc) diuji untuk mengetahui kemampuan isolat bakteri endofit sebagai pendukung pertumbuhan tanaman. Hasil penelitian menunjukkan bahwa 87,10% isolat bakteri endofit dari tanaman pisang Klutuk merupakan kelompok bakteri Gram negatif dan 82,80% (77 isolat bakteri) menunjukkan karakter tunggal atau ganda sebagai PPT. Di dalam kelompok isolat tersebut, terdapat berturut-turut 60, 38, dan 20 bakteri yang mampu memfiksasi N2, menghasilkan AIA, dan antagonisme terhadap Foc. Hasil pengujian ini menunjukkan bahwa bakteri endofit dari pisang Klutuk didominasi oleh bakteri kelompok Gram negatif yang memiliki kemampuan sebagai pendukung pertumbuhan tanaman.Abstract The role of endophytic bacteria on the biotic and abiotic resistance of Klutuk banana plants has never been reported. A total of 93 endophytic bacterial isolates were obtained from Klutuk banana plants in a previous study, but their potency as Plant Growth Promoting Bacteria (PGPB) is not elucidated. This study aims to characterize those 93 endophytic bacterial isolates. Gram staining was performed to differentiate between Gram-positive and negative bacteria among the isolates. The ability to fix nitrogen (N2), produce indole acetic acid (IAA) and antagonize Fusarium oxysporum f. sp. cubense (Foc) were also examined to determine their potency as PGPB. The results showed that 87.1% of the endophytic bacterial isolates were Gram-negative bacteria and 83.87% (78 bacterial isolates) had single or multiple traits of PGPB. Among the isolates, 60, 38, and 20 bacteria were able to fix N2, produce IAA, and antagonize Foc, respectively. The results indicated that the endophytic bacteria inhabiting Klutuk banana plant are dominated by Gram-negative PGPB.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.58677

Co-Authors A. C. Hayward Abd. Rasyid Syamsuri Ani Widiastuti, Ani Arif Wibowo Arif Wibowo Ariyanti, Nur Fitriana Christanti Sumardiyono Damanik, Nina Septania Donny Widianto Donovan, Nerida Eka Tarwaca Susila Putra Endang Sulistyaningsih Harper, Stephen Ilma, Hurin Nabila Aghnia JAKA WIDADA Khurshid, Tahir Kuwat Triyana Lestiyani, Ayu Liliek Sulistyowati M. Fegan Margo Sulistio McCann, Honour C. Mo, Jianhua Nur Edy Nurviani Nurviani Peter Taylor Prakoso, Ady Bayu Pratiwi, Annisa Hasta Ramot Christian Santika, Islaminati Anna Saraswati, Vivian Thea Sri Sulandari Sri Widyaningsih Susamto Somowiyarjo Syafitri, Luthfiana Mifta Tri Joko Tri Joko Triastuti Rahayu Triwibowo Yuwono Utik Windari Wayan T. Artama Werdiningtyas, Cyrilla Kinanti Yekti Asih Purwestri Yenny Sariasih

Title

Found 20 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 20 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 20 Documents
Search