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All Journal agriTECH Partner E-Dimas: Jurnal Pengabdian kepada Masyarakat Jurnal Teknologi Agro-Industri Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Madani : Indonesian Journal of Civil Society
Eny Idayati
Politeknik Pertanian Negeri Kupang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Engineering Environmental Science Immunology & microbiology Social Sciences Veterinary

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Potensi Senyawa Bioaktif Mesocarp Buah Lontar (Borassus fl abeliffer L.) sebagai Sumber Antioksidan Alami Eny Idayati; Suparmo Suparmo; Purnama Darmadji
agriTECH Vol 34, No 3 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.003 KB) | DOI: 10.22146/agritech.9455

Abstract

This study was aimed to characterize the physical and chemical properties of borassus palm fruit mesocarp, to determine the best type of solvent to extract bioactive compounds by maceration method, and to evaluate the antioxidant properties of bioactive compounds using DPPH (1,1-difenil-2-pikrilhidrazil) method. Carotenoid was separated based on TLC (thin layer chromatography) method, which produced spots. To confi rm the results, the spots were scanned using UV-vis spectrofotometry. The results showed that the water content of borassus fruit mesocarp was 77.31%, while total fat, ash content, and tannin were 0.11%, 1.43%, 0.08%, respectively. Total Carotenoid was 8324.6 µg/100g with β carotene content was 6217.48 microgram/100g. The best solvent used in extracting the bioactive compounds was ethanol and acetone with (1:1) ratio. The highest yield was 4.3% and bioactive compounds in palm fruit mesocarp extracts as antioxidants was about 87% as carried out by DPPH method, so it could become a potential antioxidant. TLC Identifi cation produced two spots. One spot was identifi ed as carotenoids from xanthophyll group and the other one was β-carotene.ABSTRAKPenelitian ini bertujuan untuk karakterisasi senyawa bioaktif dalam mesocarp buah lontar berdasarkan sifat fisik dan kimia, mengetahui jenis pelarut terbaik untuk mengekstrak senyawa bioaktif dengan metode maserasi, serta mengevaluasi sifat antioksidan senyawa bioaktif mesocarp dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Dasar pemisahan karotenoid dengan metoda KLT (kromatografi  lapis tipis) pada ekstrak lalu dikuatkan dengan hasil scanning menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa mesocarp buah lontar mengandung kadar air 77,31%; total lemak 0,11%, kadar abu 1,43%; tanin 0,08%; total karotenoid 8324,6 µg/100g dengan kandungan senyawa  karoten 6217,48 µg/100g. Perlakuan pelarut terbaik untuk proses ekstraksi senyawa bioaktif adalah etanol dan aseton dengan rasio (1:1). Hasil rendemen tertinggi yaitu 4,3% dan potensi senyawa bioaktif dalam ekstrak mesocarp buah lontar sebagai antioksidan dengan metode DPPH yaitu sekitar 87%, sehingga berpotensi sebagai salah satu senyawa antioksidan. Identifi kasi dengan metode KLT yang menghasilkan 2 noda yaitu noda 1 diduga karotenoid dari golongan xantofi l dan noda 2 yatu  karoten.
Akseptabilitas Es Krim dengan Fortifikasi Mesocarp Lontar (Borassus flabeliffer L.) sebagai Antioksidan dan Serat Pangan Alami Eny Idayati; Agrippina Agnes Bele; Rikka Welhelmina Sir
agriTECH Vol 38, No 3 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.78 KB) | DOI: 10.22146/agritech.22951

Abstract

Lontar palm (Borassus flabeliffer L.) fruit is one of the specific tropical fruits from East Nusa Tenggara province (NTT)-Indonesia that is rich in functional compounds such as beta-carotene as an antioxidant and food fiber.  However, the utilization of these fruits is still limited due to the streroidal saponins and pholifenol content that makes them taste bitter and less favored by consumers. Therefore, one alternative that can be done is by enriching them into ice cream products. The production of Ice cream cannot go through with high heating processing so that the antioxidants extracted from lontar palm mesocarp can be in a stable condition. The aim of this study is to investigate the effect of ice cream on the acceptability values of the hedonic test, determining of fiber and beta-carotene content and evaluating antioxidant activities fortification from the lontar's mesocarp. The amount of lontar mesocarp for fortification (F) were 30%, 40% and 50% of the wet weight with the combination of some variation of milk treatment such as full cream milk, sweetened condensed milk, and liquid soy milk. The acceptability of the product was tested using hedonic test against color, aroma, texture, and flavor parameter. Antioxidant activity was performed by determination of the DPPH value (1.1-diphenyl-2picryhydrazil radical-scavenging), dietary fiber using enzymatic-gravimetric methods and UV-Vis spectrophotometers to analyze the content of beta-carotene. The results showed that on the acceptability test, the most preferred samples valued by panelists were the fortification, F1 treatment  (30%) with the content of β-carotene of full cream milk was 2212.44 ± 11.82 μg /100 mg, dietary fiber was 8.95 ± 0.05%, and antioxidant activity was 45.54 ± 0.36%.
Co-Authors Agrippina Agnes Bele Ayu Pramita John Tibo Kana Tiri Kartiwan Kartiwan Krisna Setiawan M. Basri Naema Bora, Naema Purnama Darmadji Rikka W. Sir Rikka Welhelmina Sir Senni J. Bunga Suparmo Suparmo