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All Journal HAYATI Journal of Biosciences Bioma : Berkala Ilmiah Biologi
Akmaliyah, Rizna
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education

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Potential Probiotic Yeasts of the Pichia Genus Isolated from ‘Dadih’, a Traditional Fermented Food of West Sumatra, Indonesia Chihombori, Tatenda Calvin; Mustopa, Apon Zaenal; Astuti, Rika Indri; Mutiara, Ilma; Refli, Redoyan; Umami, Rifqiyah Nur; Fatimah; Irawan, Herman; Ekawati, Nurlaili; Trinugroho, Joko P; Akmaliyah, Rizna; Chairunnisa, Sheila; Amani, Febriyanti Nur; Manguntungi, Baso; Hertati, Ai; Mamangkey, Jendri
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.320-340

Abstract

Fermented buffalo milk, known as dadih, serves as a reservoir of potential probiotic yeasts. Over the past two decades, probiotic yeasts have gained increasing attention in both basic and clinical sciences due to their health benefits. This study aimed to isolate and characterize probiotic yeasts from dadih. Yeasts were isolated using yeast Extract, peptone, and dextrose (YPD) medium, and molecularly identified through 18S-rRNA sequencing. Probiotic potential was assessed by evaluating resistance to acidic pH, bile salts, proteolytic, lipolytic, and hemolytic activities. Secondary metabolites produced during fermentation were tested for antimicrobial properties. GBT30 and GBT37 isolates were selected based on their superior performance in probiotic property assays for further analysis. Molecular identification revealed these isolates as Pichia occidentalis (GBT30) and Pichia kudriavzevii (GBT37). Both strains demonstrated in vitro survivability under simulated gastrointestinal conditions and exhibited antimicrobial activity. Whole-genome sequencing of P. kudriavzevii GBT37 identified a genome size of 10,906,850 base pairs, distributed across four chromosomes with a GC content of 38.26%. Notably, secondary metabolite biosynthesis genes were located on contig 7. In addition, 26 probiotic-related genes, including GSY1, HSC82, HSP104, TPS1, ARN1, FLO1, ALA1, SIR2, and others, were identified in P. kudriavzevii GBT37, indicating its potential as a probiotic yeast. The traditional fermentation process of dadih offers probiotic yeasts with promising health benefits, supporting its potential as a functional food.
Exploration of Pilobolus sp. Fungi from Various Livestock Manures in Kedung Pacul Village, Klaten Mahardhika, Wahyu Aji; Akmaliyah, Rizna; Diana, Milsa Solva; Listyowati, Sri
Bioma : Berkala Ilmiah Biologi Vol. 26, No 1, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.55874

Abstract

Pilobolus is a coprophilous fungus belonging to the Zygomycota. Pilobolus sp. is called a coprophilous fungus because it can live in animal feces. The uniqueness of this fungus is that it can shoot its spores, so Pilobolus sp. is called a shotgun fungus. Pilobolus sp. exhibits a phototropism mechanism in which the sporangium shoots spores in the direction of light. Pilobolus has a unique living habitat, namely in the manure of herbivores such as cows, goats, sheep, etc. This habit of life may seem terrible to us, but fungi like Pilobolus sp. are very important in life because they are a type of decomposer, capable of breaking down organic matter from dead living things. So the purpose of this study was to observe the spores produced by the fungus Pilobolus sp.  (a fungus in animal waste). The method used was to cultivate the fungus Pilobolus sp. for seven days on various animal wastes placed in jam bottles. The results showed that the Pilobolus sp. fungus grew on various media of animal feces, such as horses, cows, goats, and pigs, with different growth times, and there were also spores shot on the glass walls that had been perforated.
Co-Authors Ai Hertati, Ai Amani, Febriyanti Nur Apon Zaenal Mustopa Chairunnisa, Sheila Chihombori, Tatenda Calvin Diana, Milsa Solva Ekawati, Nurlaili Fatimah Irawan, Herman Jendri Mamangkey Listyowati, Sri Mahardhika, Wahyu Aji Manguntungi, Baso Mutiara, Ilma Refli, Redoyan Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Trinugroho, Joko P