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All Journal HAYATI Journal of Biosciences Sains Natural: Journal of Biology and Chemistry Annales Bogorienses
Irawan, Herman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences

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Potential Probiotic Yeasts of the Pichia Genus Isolated from ‘Dadih’, a Traditional Fermented Food of West Sumatra, Indonesia Chihombori, Tatenda Calvin; Mustopa, Apon Zaenal; Astuti, Rika Indri; Mutiara, Ilma; Refli, Redoyan; Umami, Rifqiyah Nur; Fatimah; Irawan, Herman; Ekawati, Nurlaili; Trinugroho, Joko P; Akmaliyah, Rizna; Chairunnisa, Sheila; Amani, Febriyanti Nur; Manguntungi, Baso; Hertati, Ai; Mamangkey, Jendri
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.320-340

Abstract

Fermented buffalo milk, known as dadih, serves as a reservoir of potential probiotic yeasts. Over the past two decades, probiotic yeasts have gained increasing attention in both basic and clinical sciences due to their health benefits. This study aimed to isolate and characterize probiotic yeasts from dadih. Yeasts were isolated using yeast Extract, peptone, and dextrose (YPD) medium, and molecularly identified through 18S-rRNA sequencing. Probiotic potential was assessed by evaluating resistance to acidic pH, bile salts, proteolytic, lipolytic, and hemolytic activities. Secondary metabolites produced during fermentation were tested for antimicrobial properties. GBT30 and GBT37 isolates were selected based on their superior performance in probiotic property assays for further analysis. Molecular identification revealed these isolates as Pichia occidentalis (GBT30) and Pichia kudriavzevii (GBT37). Both strains demonstrated in vitro survivability under simulated gastrointestinal conditions and exhibited antimicrobial activity. Whole-genome sequencing of P. kudriavzevii GBT37 identified a genome size of 10,906,850 base pairs, distributed across four chromosomes with a GC content of 38.26%. Notably, secondary metabolite biosynthesis genes were located on contig 7. In addition, 26 probiotic-related genes, including GSY1, HSC82, HSP104, TPS1, ARN1, FLO1, ALA1, SIR2, and others, were identified in P. kudriavzevii GBT37, indicating its potential as a probiotic yeast. The traditional fermentation process of dadih offers probiotic yeasts with promising health benefits, supporting its potential as a functional food.
Truncation on N-Terminal Hydrophobic Domain of L1 Major Capsid Protein of Human Papillomavirus Type 52 Enhances Its Expression in Hansenula polymorpha Arifah, Rosyida Khusniatul; Firdaus, Moh Egy Rahman; Chairunnisa, Sheila; Irawan, Shasmita; Ekawati, Nurlaili; Irawan, Herman; Nurfatwa, Maritsa; Hertati, Ai; Swasthikawati, Sri; Novianti, Ela; Mustafawi, Wike Zahra; Nur Umami, Rifqiyah; Mustopa, Apon Zaenal
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1062-1072

Abstract

Human papillomavirus (HPV) infection is the main cause of cervical cancer. The administration of the HPV prophylactic vaccine, which is commonly produced based on HPV L1 major capsid protein, significantly reduces the incidence of cervical cancer. However, the coverage of the HPV vaccination program is often hindered due to its relatively high cost. This study aimed to evaluate the impact of N-terminal hydrophobic domain truncation on the expression of L1 major capsid protein of HPV type 52 in Hansenula polymorpha. The truncation enhanced the yield of L1 protein expression compared with the full length, which was confirmed by Western blot and ELISA. Furthermore, the truncated L1 protein formed virus-like particles (VLPs), which were confirmed by transmission electron microscopy (TEM). Bioinformatics analysis showed that the truncated L1 protein was more soluble compared with the full length, possibly increasing the protein expression. These findings could pave the way for the development of a more cost-effective HPV type 52 L1 protein production in H. polymorpha to be used as a VLP-based prophylactic vaccine.
Medium Optimization for Recombinant Human Papillomavirus Type 52 L1 Protein Production in Pichia pastoris GS115 Platform on Bioreactor Scale Mustopa, Apon Zaenal; Nur Amani, Febriyanti; Irawan, Herman; Novianti, Ela; Swasthikawati, Sri; Ekawati, Nurlaili; Nurfatwa, Maritsa; Joko Wahyono, Daniel; Juanssilfero, Ario Betha; Mamangkey, Jendri; Purnomo, Yudi; Hertati, Ai; Wijaya, Hans; Dewi, Kartika Sari; Ningrum, Ratih Asmana
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1283-1294

Abstract

Human papillomavirus (HPV) stands as the primary etiological agent in the development of invasive cervical cancer worldwide. The L1 protein is a pivotal constituent of prophylactic HPV vaccines. Notably, HPV type 52 is one of the most prevalent genotypes found in squamous cell carcinoma cases in Indonesia. This research endeavor aims to enhance the productivity of recombinant HPV-52 L1 protein by optimizing the culture conditions of P. pastoris GS115 cells. In this study, we conducted trials employing 17 different media variants to optimize the expression of recombinant HPV-52 L1 protein. The results from small-scale experiments revealed three media, namely SYN6.10, BMMY, and SYN6.1, which exhibited promising yields of recombinant HPV-52 L1 protein as assessed through ELISA or immunoassay analysis. We succeeded in refining the SYN6.10 derivative, denoted as SYN6.10b, specifically designed for use in 1-L and 5-L bioreactors. This achievement was realized by adjusting Trace Element Solution (TES) and Vitamin Solution (VS) concentrations and implementing a methanol fed-batch phase with the addition of 0.3% methanol after 24 and 48 hours of fermentation in the P. pastoris medium. Further visualizations through SDS-PAGE and western blot analysis confirmed the protein after 72 hours of fermentation in a 1-L bioreactor using the SYN6.10b medium. In conclusion, the SYN6.10b medium required a 72 hours fermentation period to successfully express recombinant HPV-52 L1 protein in the P. pastoris platform.
Instant Granule Formulation Combining White Sweet Potato Leaves Extract (Ipomoea batatas (L.) Lam.) and Javanese Chili (Piper retrofractum Vahl) Irawan, Herman; Permata Sari, Indah; Tisnadjaja, Djadjat; Ekawati, Nurlaili; Hertati, Ai; Novianti, Ela; Masaenah, Eem
Sains Natural: Journal of Biology and Chemistry Vol. 14 No. 1 (2024): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v14i1.612

Abstract

Dengue haemorrhagic fever is a viral infection transmitted by the Aedes aegypti mosquito, which is one of the symptoms is thrombocytopenia. The extract from white sweet potato leaves is recognized for its potential to increase the number of platelet count. This research aimed to develop a formula for an instant granule combining white sweet potato leaves extract with Javanese chili extract meeting taste preferences and societal acceptability. The preparation of the extract was performed by maseration using 70% ethanol and then concentrated with a rotary evaporator. The result was a thick extract which was then dried in an oven at 50℃. The manufacture of instant granule formulations was conducted using wet granulation method. Various formulations were obtained by differentiated concentration of Javanese chili used in the formula (FI 1%, FII 2%, and FIII 3%). The instant granule formulation was evaluated by organoleptic assay and other essential parameters for instant granule, such as moisture content, flow rate, angle of repose, and particle size distribution. The FII 2% formulation emerged as the most acceptable with an average score of 2.17. Non-parametric analysis indicated no significant difference among the three formulas, as the obtained significant value (α) was ≥ 0.05, leading to the acceptance of the null hypothesis H0.
Mini Review: Prostate Cancer Diagnosis and Therapy Fathurahman, Alfi Taufik; Swasthikawati, Sri; Irawan, Herman; Supriatna, Dadang; Wardiana, Andri
Annales Bogorienses Vol. 25 No. 2 (2021): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cancer, a non-communicable illness, is the leading cause of death worldwide. In 2030, cancer is expected to exceed 21 million cases and 13 million cancer deaths globally. One of the most prevalent and significant types is prostate cancer (PCa). It is commonly associated with adenocarcinoma, which develops from the mucous glands within the organ. This review highlights available detection systems, and therapeutic options in PCa management. One prevention of deadly PCa is early diagnoses, such as prostate-specific antigen (PSA) screening or genomic profiling. Further testing like MRI or CT scan may also be needed to detect cancers that have progressed to other body regions. There are several possible treatments for PCa, including watchful cancer waiting, surgery, radiotherapy, hormone therapy, and chemotherapy. Based on current studies, androgen deprivation therapy (ADT) combined with docetaxel therapy enhanced great results to treat advanced PCa. The latest development, called theranostics, is a single entity that can perform both diagnostic and therapeutic functions. It can detect disease borders, track therapy in real-time, and provide prognostic data. The FDA has already authorized two prostate-specific membrane antigen (PSMA) positron emission tomography (PET) devices: including Gallium 68 PSMA-11 (Ga 68 PSMA-11) and Pylarify (piflufolastat F 18).
Co-Authors Ai Hertati, Ai Akmaliyah, Rizna Amani, Febriyanti Nur Andri Wardiana, Andri Apon Zaenal Mustopa Arifah, Rosyida Khusniatul Ario Betha Juanssilfero Chairunnisa, Sheila Chihombori, Tatenda Calvin Dadang Supriatna Ekawati, Nurlaili Fathurahman, Alfi Taufik Fatimah Firdaus, Moh Egy Rahman Irawan, Shasmita Jendri Mamangkey Joko Wahyono, Daniel Kartika Sari Dewi Manguntungi, Baso Masaenah, Eem Mustafawi, Wike Zahra Mutiara, Ilma Novianti, Ela Nur Amani, Febriyanti Nur Umami, Rifqiyah Nurfatwa, Maritsa Permata Sari, Indah RATIH ASMANA NINGRUM Refli, Redoyan Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Sri Swasthikawati, Sri Tisnadjaja, Djadjat Trinugroho, Joko P Wijaya, Hans Yudi Purnomo