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All Journal HAYATI Journal of Biosciences POSITRON
Abdillah, Oktaviardi Bityasmawan
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy Physics

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Electrochemically Exfoliated Graphite/Cu/Cu2O Composites and Their Photocatalytic Activity Destiarti, Lia; Abdillah, Oktaviardi Bityasmawan; Maharsi, Retno; Floweri, Octia; Iskandar, Ferry
POSITRON Vol 10, No 1 (2020): Vol. 10 No. 1 Edition
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Univetsitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.126 KB) | DOI: 10.26418/positron.v10i1.37260

Abstract

In this work, the photocatalytic performance of electrochemically exfoliated graphite (EG) with low copper addition (≤ 5 wt.%) was studied. Composites of EG/Cu/Cu2O were successfully prepared by microwave-assisted in situ reduction method. FTIR spectra of the samples showed that the main functional groups of graphite were detected in the samples. XRD characterization further proved the presence of EG, Cu, and Cu2O in the samples. The higher proportion of Cu2O presented in the samples prepared with a higher amount of Cu2+. SEM analysis showed that Cu2O/Cu particles were homogeneously deposited on the surface of EG. The composites of EG, Cu, and Cu2O with a varied amount of Cu (1 and 5 wt. %) in EG / Cu2+ mixture were examined as photocatalyst in the degradation process of Rhodamine B (RhB). The photocatalytic degradation of RhB was analysed by observing its decolorization within a set time of irradiation. UV-Vis analysis revealed that the degradation of RhB in EG/Cu/Cu2O A and B for 105 minutes was 26 and 35 %, respectively. The result demonstrates that the sample with a larger amount of Cu2O (sample B, Cu 5 wt.%) shows higher photocatalytic activity in the degradation of RhB.
Development of a Fluorescence Immunoassay Based on Curcumin Carbon Dots-labeled IgY Antibodies for SARS-CoV-2 Detection Rachmadani, Nisa Amanda; Soetomo, Meilisa Keizia; Taharuddin, Audrey Angelina Putri; Santika, Arum Sinda; Abdillah, Oktaviardi Bityasmawan; Permatasari, Fitri Aulia; Iskandar, Ferry; Rachmawati, Heni; Fibriani, Azzania
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.44-57

Abstract

Carbon dots (CDs) are widely utilized in biomedical applications as fluorescent labels for imaging and diagnostics due to their excellent biocompatibility and superior optical properties. These advantages often make CDs a substitute for organic fluorescent dyes, which suffer from low emission intensity and poor photostability when interacting with biomolecules. Moreover, carbon-based materials are eco-friendly and can be synthesized from natural sources, such as curcumin, a chromophore compound abundantly available in Indonesia. Therefore, this study conducted a preliminary investigation on curcumin CDs-labeled IgY antibodies (IgY-cur CDs) for fluorescence immunoassay of SARS-CoV-2 in rapid test applications. The synthesis of the fluorescent label involved a carbodiimide coupling reaction using EDC/NHS agents to conjugate IgY antibodies with curcumin CDs. The IgY-cur CDs conjugate was confirmed to detect antigens through FRET immunosensor mechanisms, showing a significant increase in fluorescence intensity with increasing antigen concentrations (p < 0.05), with a minimum sample concentration of 10 ng. Furthermore, the IgY-CDs cur conjugate was applied as a reporter in a fluorescence-based LFIA using a sandwich assay format. The test strip successfully detected synthetic multiepitope SARS-CoV-2 antigens with an estimated detection limit of 54.28 µg and nasopharyngeal samples from confirmed COVID-19 patients within 35 minutes of operation. The test strip was evaluated to be stable under cold storage at 4°C for up to 3 weeks. In conclusion, curcumin CDs-labeled IgY antibodies demonstrate promising potential for further development as fluorescent labels in rapid diagnostic applications targeting SARS-CoV-2.
Co-Authors Destiarti, Lia Ferry Iskandar Fibriani, Azzania Floweri, Octia HENI RACHMAWATI Maharsi, Retno Permatasari, Fitri Aulia Rachmadani, Nisa Amanda Santika, Arum Sinda Soetomo, Meilisa Keizia Taharuddin, Audrey Angelina Putri