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Haryatmi, Dwi
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Incidence Rates of Pediculosis Capitis and Helminthiasis in Children in Danukusuman Surakarta Setya, Adhi Kumoro; Haryatmi, Dwi; Listiyaningsih, Alfina; Putri, Elisa
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6956

Abstract

Pediculosis capitis and helminthiasis It is categorized as a neglected disease but is still an important health problem, especially for children. These two diseases each cause clinical disorders on the surface and internal organs of the body. Danukusuman is one of the most densely populated areas in the city of Surakarta, where in previous years many cases of gastrointestinal disorders affected local residents. The aim of this study to determine the incidence of pediculosis capitis and helminthiasis in elementary school children in the city of Surakarta. This research uses a descriptive analysis research design. The population in the study were children aged 6-8 years in Danukusuman Surakarta totaling 100 respondents. Samples were taken using total sampling technique. The diagnosis of pediculosis capitis is made on the skin and hair area of the head and intensity helminth enforced use method Kato katz from fecal samples by calculating the number of worm parasite eggs per gram of feces (EPG). Based on research conducted on 100 respondents, the results showed that 29 (29%) respondents had pediculosis capitis and cases of helminthiasis showed that 2 respondents (2%) had Ascari  lumbricoides and Trichuris trichiura with a total of 24 epg of parasites each with a light infection intensity. From the results of this study, it was reported that the incidence of pediculosis capitis in children in Danukusuman was 29% and helminth infections were 2% of cases with a mild degree of infection.
Description of Candida Species Infection on the Skin Between the Fingers of Milkfish Selectors Mahanani, Zhendyta Elsya; Haryatmi, Dwi
Indonesian Journal of Global Health Research Vol 5 No 4 (2023): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v5i4.2418

Abstract

Candidiasis is a fungal infection of the skin caused by the Candida fungus. Candida fungi live in the human body as saprophytes which can be in the digestive tract, respiratory tract and vagina of normal or healthy people. Candida infections on the skin between the fingers most often occur when the hands are repeatedly soaked in water for long periods of time, this occurs in housemaids, cooks, vegetable and fish handlers. The aim of this research was to determine whether there was skin candidiasis infection between the fingers of milkfish sorters in Mojolaban Sukoharjo and what species of Candida were found. The research used a descriptive method with the population of milkfish sorting workers taken by purposive sampling based on criteria in accordance with the objectives of the examination which obtained a total of 14 respondents. Data was taken from the results of questionnaires and skin scraping cultures on CHROMagar-Candida media which were then identified macroscopically and microscopically. The results were presented in tabular form and analyzed descriptively, where 3 of 14 skin scraping samples from milkfish sorters in Mojolaban Sukoharjo were found (positive) to be infected with Candida krusei.
Prevalence of Pediculosis Capitis in Orphanages and Islamic Boarding Schools in the Surakarta Setya, Adhi Kumoro; Haryatmi, Dwi
Indonesian Journal of Global Health Research Vol 5 No 4 (2023): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v5i4.2484

Abstract

Residential environments such as orphanages and identical boarding houses have quite high population densities. This makes cottages and orphanages vulnerable and has a high potential for transmission of Pediculus Humanus var. capitis causes the disease Pediculosis capitis. This disease is an indicator of poor personal hygiene and the environment in which one lives. The higher the case of tick infestation, the worse the surrounding community becomes. This study aims to determine the prevalence of cases of pediculosis capitis in Islamic boarding schools and orphanages in the Surakarta.This research uses a descriptive research design with purposive sampling technique. The samples analyzed were the hair and scalp areas of 155 child and adolescent respondents in Islamic boarding schools and orphanages in Surakarta City. The data obtained was carried out by laboratory examination in the form of egg, nymph and adult flea stages as well as questionnaires filled out by respondents using guided interviews in the form of data on respondents' characteristics and personal hygiene status obtained through scoring. The relationship between Personal Hygiene and the incidence of Pediculosis capitis was analyzed using the chi-square test. From the results of research conducted on all respondents who were willing and met the criteria, it was discovered that of the 155 respondents, 59 (38%) were positive for pediculosis capitis and 96 (62%) did not experience pediculosis capitis. Sequentially, the highest cases were found in locations; Laweyan sub-district orphanage (76.7%), city orphanage (60%), Pasar Kliwon Islamic boarding school (36%) and Laweyan orphanage (0%). The personal hygiene status of the respondents in this case was 81% good, while 19% had poor personal hygiene status. In all cases that were found to be positive for pediculosis capitis, all of them were found to be in the egg, nymph and adult stages. The highest characteristics of positive cases were found in respondents with straight hair and longer than shoulder length. From this research, it was concluded that there was a significant relationship between personal hygiene and the cases of pediculosis capitis found. The point prevalence of pediculosis capitis in Islamic boarding schools and orphanages in the city of Surakarta is 38% of cases. As many as 81% of respondents had good personal hygiene status and 19% of respondents had poor personal hygiene.
DNA Quality Testing of Isolated Stored Giemsa Blood Smear Samples using Geneius™ Micro gDNA Extraction Kit as An Alternative Sample for Malaria Molecular Examination Saroh, Dewi; Haryatmi, Dwi; Widodo, Rendyansah Agung
Indonesian Journal of Global Health Research Vol 7 No 1 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i1.4844

Abstract

The gold standard for malaria examination is microscopic examination. However, microscopic examination has many shortcomings, namely the difficulty of distinguishing between Plasmodium sp. species when mixed infections occur. Other malaria examinations for screening can be done with RDT. However, it also has shortcomings, namely the minimum number of parasites that can be detected is 100 parasites/ul. Molecular examination is the most sensitive and accurate examination in the diagnosis of malaria. The initial stage of molecular examination is the DNA isolation stage. This DNA isolation is very important in determining the success of molecular examination. Isolation of Plasmodium sp. DNA using blood smear samples is still rarely done and there is no standard procedure for isolating DNA from blood smears. The purpose of this study was to determine whether stored Giemsa blood smears can be used as samples in DNA isolation in malaria cases. The method used in isolation is using the Geneius™ Micro gDNA Kit. The results of the study were that stored Giemsa blood smear samples of Plasmodium vivax for 3 months could be isolated using the Geneius™ Micro gDNA Kit and could be used as DNA templates for amplification with target gene primers.
Comparative Analysis of Staining Time Using Giemsa 10% on the Results of Malaria Blood Preparations Wirawan, M. Richo Realdy; Haryatmi, Dwi
Indonesian Journal of Global Health Research Vol 7 No 4 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i4.6347

Abstract

Malaria is a parasitic infectious disease that remains a significant public health problem in Indonesia, particularly in endemic areas. Rapid and accurate diagnosis of malaria is essential to prevent serious complications. The 10% Giemsa staining method is a rapid technique for microscopic examination of malaria, but the staining time standard needs to be validated for each batch of staining solution used in local laboratories. This study aims to analyze the comparison of malaria blood smear staining results using 10% Giemsa with different staining durations of 10 minutes, 20 minutes, and 30 minutes at Natar Medika Hospital. This research employed a cross-sectional approach with observational analysis of thin blood smears from 15 malaria-positive blood samples. The blood smear evaluation used a scoring system: a score of 0 was given for poor staining results (unclear background, unstained or non-blue cytoplasm, and unstained or non-red nucleus), and a score of 1 for good staining results (clear background, blue cytoplasm, and red nucleus). The results showed that staining for 20 minutes produced the best quality thin blood smears, characterized by a clear background, blue cytoplasm, and red nucleus. Statistical analysis using the Kruskal-Wallis test indicated significant differences between staining durations (p < 0.05). Therefore, staining for 20 minutes using 10% Giemsa is recommended to obtain optimal microscopic results in malaria diagnosis at Natar Medika Hospital.
Sensitivity and Specificity of Malaria Rapid Diagnostic Test (RDT) Yuniatun, Siti Retno; Haryatmi, Dwi
Indonesian Journal of Global Health Research Vol 7 No 4 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i4.6362

Abstract

Malaria remains a significant public health issue in many regions, and early diagnosis is crucial for effective treatment. Rapid Diagnostic Tests (RDT) are commonly used for malaria detection due to their speed and ease of use, but their diagnostic accuracy, particularly in regions with varying levels of parasitemia, remains a subject of ongoing research. This study aimed to evaluate the performance of Rapid Diagnostic Tests (RDT) for malaria diagnosis at Puskesmas Hanura, Teluk Pandan District, Pesawaran Regency, by assessing the sensitivity and specificity of the test. Methods: This analytical observational study employed a cross-sectional approach, analyzing blood samples from malaria suspect patients collected between December 2024 and February 2025, with a total sample size of 50 samples. The diagnostic performance of RDT was compared to the gold standard of microscopy using thick and thin blood smears. Sensitivity and specificity were calculated based on the results of both methods. The sensitivity of the RDT was found to be 93.5%, indicating that the test was highly effective in detecting true positive malaria cases, particularly in individuals with high parasitemia. The specificity was 100%, demonstrating that the RDT accurately identified malaria-free individuals with no false positive results. However, the study also identified two false negative cases, suggesting that the RDT's sensitivity could be reduced in cases of low parasitemia. The Rapid Diagnostic Test demonstrated excellent sensitivity and specificity in detecting malaria at Puskesmas Hanura, with no false positive results and a high rate of true positive detection. However, the test showed some limitations in detecting low parasitemia, emphasizing the need for confirmatory diagnostic techniques, such as microscopy or molecular methods, especially in areas with low transmission or mild infections.
Identifikasi Spesies Plasmodium Malaria Pada Masyarakat di Kabupaten Cilacap Provinsi Jawa Tengah Identification of Plasmodium Malaria in Communities in Cilacap, Central Java Province Sugesti, Aprilia; Haryatmi, Dwi
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 4 No. 2 (2022): Volume 4, Nomor 2, November 2022
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v4i2.301

Abstract

Latar belakang : Malaria merupakan masalah kesehatan masyarakat yang dapat menyebabkan kematian terutama pada kelompok risiko tinggi seperti bayi, balita, ibu hamil, dan secara langsung menyebabkan anemia yang menurunkan produktivitas kerja. Pengendalian malaria dilakukan secara menyeluruh melalui upaya promotif, preventif, pengobatan, dan rehabilitasi yang ditujukan untuk menurunkan angka kesakitan dan kematian serta mencegah Kejadian Luar Biasa (KLB). Pada tahun 2010 terjadi KLB di kabupaten Cilacap mencapai 45 kasus yang terjadi di Kecamatan Kampung Laut. Kasus yang terjadi di Kampung Laut diakibatkan penularan setempat dan juga impor. Tujuan Peneltian : Penelitian ini bertujuan untuk mengidentifikasi ada tidaknya Plasmodium malaria beserta spesies Plasmodium malaria pada sediaan tetes darah tebal dan tipis pada masyarakat di Kabupaten Cilacap. Metode : Metode penelitiannya adalah deskriptif dengan teknik pengambilan sampel Accidental Sampling (non random) dan dilakukan pemeriksaan Laboratorium. Sampel dalam penelitian ini sebanyak 8 responden yang memiliki gejala klinis malaria di delapan puskesmas di wilayah Kabupaten Cilacap. Hasil : Hasil penelitian identifikasi jenis Plasmodium yang menginfeksi pada kejadian Malaria di Kabupaten Cilacap adalah Plasmodium falciparum (50%) dan Plasmodium vivax (50%) dari keseluruhan jumlah sampel dan semua yang terinfeksi malaria dikarenakan setelah berpergian atau bekerja dari daerah endemis malaria (malaria impor). Kesimpulan : Jenis Plasmodium yang menginfeksi pada pemeriksaan sediaan tetes darah tebal dan tipis penderita malaria adalah Plasmodium falciparum dan Plasmodium vivax. Bagi masyarakat diharapkan menggunakan informasi ini dengan bijak agar bisa membantu dan berpartisipasi aktif dalam penanggulangan penyakit malaria di Kabupaten Cilacap Provinsi Jawa tengah.
Identifikasi Soil Transmitted Helminth (STH) Pada Petugas Sampah di Kecamatan Ciputat Kota Tangerang Selatan Jannah, Rianda Miftahul; Haryatmi, Dwi
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 5 No. 2 (2023): Volume 5, Nomor 2, November 2023
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v5i2.319

Abstract

Indonesia termasuk negara yang memerlukan penanganan khusus terhadap kecacingan. penyakit kecacingan adalah suatu penyakit yang diakibatkan oleh infeksi cacing dari kelompok Soil Transmitted Helminths (STH) yang termasuk dalam kelompok cacing dengan siklus hidup dan penularan melalui tanah. Petugas pengangkut sampah merupakan golongan pekerja yang lebih sering melakukan kontak dengan tanah, sampah dan lebih sering berada di lingkungan yang kotor. Personal Hygiene yang buruk seperti tidak mencuci tangan sebelum makan, tidak menggunakan alas kaki saat bekerja, kebiasaan buang air besar sembarangan, merupakan faktor pendukung seseorang dapat terkontaminasi Soil Transmitted Helminths (STH). Oleh karena itu petugas pengangkut sampah sangat rentan terkena penyakit-penyakit yang penularannya melalui tanah salah satunya adalah kecacingan. Penelitian ini bertujuan untuk memberikan informasi mengenai adanya kontaminasi telur Soil Transmitted Helminths (STH) pada petugas sampah di Kecamatan Ciputat, Kota Tangerang Selatan. Desain penelitian yang digunakan yaitu deskriptif analitik dengan teknik purposive sampling. Pemeriksaan laboratorium dilakukan dengan menggunakan metode indirect tinja dengan pengendapan formalin eter. Pemeriksaan feses memberikan hasil bahwa sebanyak 6,7% atau sebanyak 2 orang responden positif terdapat telur cacing STH pada feses dengan Trichuris trichiura dan Ascaris lumbricoides. Sehingga penelitian ini menunjukkan bahwa terdapat infeksi STH pada petugas sampah di Kecamatan ciputat, Kota Tangerang Selatan.
Co-Authors Asmarani, Dewi Prantiannita Putri Budiati, Srini elisa putri Heni Pujiastuti Imam Sucahyo Janah Sayekti, Fitria Diniah Jannah, Rianda Miftahul Listiyaningsih, Alfina Mahanani, Zhendyta Elsya Margareta, Rany Gina Mulya Aisyah Noviyanti, Mike Puji Hastuti Saroh, Dewi Setya, Adhi Kumoro Sugesti, Aprilia Waritsini, Belinda Khoirul Widodo, Rendyansah Agung Wirawan, M. Richo Realdy Yuniatun, Siti Retno Yunus, Elga