Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Biospecies Akta Agrosia
Siallagan, Maria Denada
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
The Potential of Bacillus sp.’s Isolate of Coffee Beans as Plant Pest Control Candidates Ekowati, Christina Nugroho; Siallagan, Maria Denada; Sumardi, Sumardi; Rosa, Emantis
Akta Agrosia Vol 25 No 2 (2022)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Long-term use of chemical pesticides in high doses might result in chemical residues. Therefore, eco-friendly plant pest control should be getting more attention. To do so, one might consider the utilization of microorganisms that have the enzymatic ability to destroy the pest's body structure. This study aimed to explore the isolates of Bacillus sp. from coffee beans as a plant pest control candidate. Bacillus could be considered due to its ability to produce protein crystals and extracellular enzymes. Three isolates are obtained from isolation from coffee beans: Bacillus sp. 1; Bacillus sp. 2; and Bacillus sp. 3. These isolates have the enzymatic character of protease, chitinase, and lipase. The results of the bioassay test on larvae of Plutella xylostella (Lepidoptera order) showed that these isolates were able to cause larvae death within 48 hours.  
DETEKSI KRISTAL PROTEIN PADA ISOLAT BACILLUS SP. DENGAN PEWARNAAN COOMASSIE BRILLIANT BLUE Siallagan, Maria Denada; Ekowati, Christina Nugroho; Sumardi, Sumardi; Rosa, Emantis
Biospecies Vol. 13 No. 2 (2020): Juli 2020
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v13i2.9562

Abstract

Bacillus is one of the genera of bacteria that has the potential to be developed in the field of biotechnology. Bacillus is able to produce protein crystals during the stationary phase in its life cycle. This protein crystal is a protoxin that is active will be pathogenic in insects. In this study, protein crystal staining was carried out using coomassie brilliant blue dye on the isolate of Bacillus sp. with isolate code B1, B2, and B3. Before being stained, the three isolates were bred on Nutrient Broth (NB) medium at pH 5, pH 7, and pH 9, then incubated for 72 hours. Based on research results, the three isolates were detected to produce protein crystals. Crystal protein of Bacillus sp. on media with a pH of 5 and pH 7 located at the end of the spore, while the protein crystal of Bacillus sp. isolate on media with a pH of 9 located on the outside of the spores.
Co-Authors Christina Nugroho Ekowati, Christina Nugroho Rosa, Emantis Sumardi .