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All Journal Jurnal Ilmu Pertanian Indonesia Biospecies Biosfer: Jurnal Tadris Biologi Jurnal Penelitian Pertanian Terapan OLDI (Oseanologi dan Limnologi di Indonesia) Bioma : Jurnal Biologi dan Pembelajaran Biologi Akta Agrosia Jurnal Kedokteran Hewan
Christina Nugroho Ekowati, Christina Nugroho
Jurusan Biologi FMIPA Universitas Lampung, Bandar Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Uji Daya Hidup Bakteri Asam Laktat Dari Usus Itik Pada Media Tumbuh Dengan Penambahan Variasi Konsentrasi Molasis Sutrisna, Rudy; Ekowati, Christina Nugroho; Damayanti, Riska
Jurnal Penelitian Pertanian Terapan Vol 16, No 1 (2016)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.422 KB) | DOI: 10.25181/jppt.v16i1.74

Abstract

This study aims to determine differences in the viability of Lactic Acid Bacteria (LAB) isolates on MRS medium supplemented with various concentrations molasses. Concentration variation molasis used as controls were 0%, 1%, 2% and 3%. Variation of time incubation on each of the media that is 0 hours, 24 hours, 48 hours and 72 hours. The viability of each isolate was viewed with the population of Log (cells/ml) which grow on the medium and the number of generations and time of generations. The results showed that the concentration molasses added to MRS affect the viability of LAB. Concentration of 1% makes LAB better viability than controls. Total population continues to increase at the end of the incubation with a yield of 1.17 generations in time 20.46 hours/generation. In contrast to controls, reduced population size at the end of the incubation so as not to produce a generation. LAB viability in addition molasses 2% and 3% live on 48 hours of incubation. This is indicated by the number of population increased at 48 hours of incubation, and then decreased at the end of incubation. The addition of concentration variation in the types of bacteria showed different viability. At B1 adding molasses 2% and 3% live on 48 hours of incubation and control can only live on a 24 hours incubation, whereas the B3, the control can remain alive at 48 hours of incubation. In contrast to the controls on B4 can survive on 48 hours of incubation, whereas the addition of 3% molasses can only live on a 24 hours incubation. Key words : Lactic Acid Bacteria/LAB, Molasses,  Viability
PENGARUH PH TERHADAP PRODUKSI ANTIBAKTERI OLEH BAKTERI ASAM LAKTAT DARI USUS ITIK Sutrisna, Rudy; Ekowati, Christina Nugroho; Sinaga, Edelina Sinaga
Jurnal Penelitian Pertanian Terapan Vol 15, No 3 (2015)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v15i3.135

Abstract

This study aimed to determine the effect of pH on the production of antibacterial by lactic acid bacteria. This study uses a completely randomized design factorial 3X 5 (3 X 5 treatment of bacterial isolates pH) with three replications. The first factor consisted of pH 4, pH 5, pH 6, pH 7, pH 8, and the second factor that isolates B2, B7, B8, whereas the parameters consists of a clear zone. The amount of production is determined by the size of the diameter of antibacterial inhibition zone against Salmonella sp. Differences in the production of antibacterial determined based on the results of analysis of variance. The treatment significantly further test BNT at 5% level. The results showed that the effect of media pH test based on the potential for the production of antibacterial effect. The diameter of the largest clear zone produced by B7 isolates at pH 6 was 17.2 mm, 17.1 mm B8 and B2 at pH 7 at 16.7 mm. The smallest diameter of the clear zone produced by B8 at pH 4 was 15.1 mm, B2 at pH 6 there is a clear zone of 12.7 mm and isolate B7 at pH 5 of 11.5 mm Keywords: Antibacterial, Lactic Acid Bacteria, pH, Salmonella sp, Clear zone
KARAKTERISASI ENZIM XILANASE DARI ISOLAT BACILLUS SP. UJ131 DI HUTAN MANGROVE MARGASARI LAMPUNG TIMUR SEBAGAI KANDIDAT PROBIOTIK Sumardi, Sumardi; Farisi, Salman; Ekowati, Christina Nugroho; Hairisah, Suminta Frida
OLDI (Oseanologi dan Limnologi di Indonesia) Vol 4, No 3 (2019)
Publisher : Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oldi.2019.v4i3.201

Abstract

Characterization of Xilanase Enzyme by Bacillus sp. UJ131 A Probiotic Candidate from the Mangrove of Margasari, East Lampung. Mangrove are known to have high biodiversity as a potential location for exploration of enzyme-producing bacteria, one of which is the xylanase enzyme. Xylanase is an enzyme that hydrolyzes the xylan-rich hemicellulose substrate. This research aims to produce and know the character of xylanase enzyme by Bacillus sp. UJ131 isolate which was isolated from the oriental shrimp in Margasari mangrove forest of East Lampung. Xylanase enzymes are produced from the bacteria by growing them in sea water complete (SWC) broth containing xylan beechwood. Characterization xylanase enzyme consist of pH, temperature, and metal ions. The results showed that xylanase had an optimal time of 18 hours with xylanase activity of 0.18 U/ml, and optimally at pH 6 and temperature 70°C. The addition of Mn2+ and Ca2+ ions increase enzyme activity, but Fe3+, Cu2+ and Mg2+ ions decrease xylanase enzyme activity. At a maximum reaction speed of 0.25 U/ml, the xylanase enzyme can produce 1.30 ?M xylose per minute
AKTIVITAS ENZIM HIDROLASE PADA PENAPISAN ISOLAT ACTINOMYCETES TOLERAN SALINITAS Sumardi, Sumardi; Qatrunada, Vanya; Farisi, Salman; Arifiyanto, Achmad; Ekowati, Christina Nugroho; Farisi, Salman
BIOMA Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v6i1.3548

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.
The Potential of Bacillus sp.’s Isolate of Coffee Beans as Plant Pest Control Candidates Ekowati, Christina Nugroho; Siallagan, Maria Denada; Sumardi, Sumardi; Rosa, Emantis
Akta Agrosia Vol 25 No 2 (2022)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Long-term use of chemical pesticides in high doses might result in chemical residues. Therefore, eco-friendly plant pest control should be getting more attention. To do so, one might consider the utilization of microorganisms that have the enzymatic ability to destroy the pest's body structure. This study aimed to explore the isolates of Bacillus sp. from coffee beans as a plant pest control candidate. Bacillus could be considered due to its ability to produce protein crystals and extracellular enzymes. Three isolates are obtained from isolation from coffee beans: Bacillus sp. 1; Bacillus sp. 2; and Bacillus sp. 3. These isolates have the enzymatic character of protease, chitinase, and lipase. The results of the bioassay test on larvae of Plutella xylostella (Lepidoptera order) showed that these isolates were able to cause larvae death within 48 hours.  
Uji Daya Antibakteri Isolat Bakteri Asam Laktat dari Kefir terhadap Bacillus sp. dan Escherichia Coli Ningsih, Agung Setia; Ekowati, Christina Nugroho; Sumard, Sumard; Farisi, Salman
Biosfer: Jurnal Tadris Biologi Vol 9 No 2 (2018): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v9i2.2608

Abstract

Penelitian ini bertujuan untuk mengetahui daya antibakteri  yang dihasilkan oleh isolat bakteri asam laktat dari kefir susu sapi dan susu kedelai terhadap Escherichia coli. Penelitian ini dilakukan dalam empat tahapan yaitu isolasi dan identifikasi BAL kefir, produksi zat antibakteri dari isolat, menguji daya hambat isolat antibakteri BAL terhadap Escherichia coli. Hasil penelitian menunjukan Keenam Isolat bakteri asam laktat dari kefir susu kedelai dan kefir susu sapi mampu menghambat pertumbuhan Escherichia coli dalam kondisi asam dan netral. Dalam kondisi asam luas zona hambat KE1-KE3 berturut-turut adalah 3,51; 2,93; 2,66 dan SA1-SA3 adalah 1,83; 2,46; 2,46. Dalam kondisi netral zona hambat KE1-KE3 berturut-turut adalah 1,59; 2,33; 1,99 dan SA1-SA2 adalah 1,49; 2,49; 2,39.
Isolasi dan Identifikasi Jamur Entomopatogen Sebagai Kandidat Bioinsektisida Lalat Rumah (Muscadomestica) Septiana, Nofita; Rosa, Emantis; Ekowati, Christina Nugroho
Biosfer: Jurnal Tadris Biologi Vol 10 No 1 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v10i1.4083

Abstract

Lalat rumah (M. domestica) merupakan vektor mekanik berbagai penyakit oleh mikroba patogen antara lain Salmonella penyebab demam tifoid, Shigella penyebab disentri, dan E. coli penyebab diare. Pengendalian M. domestica umumnya menggunakan insektisida sintetis, namun menimbulkan resistensi dan berdampak buruk bagi lingkungan. Oleh karena itu, perlu dilakukan pengendalian alternatif berupa pengendalian biologi menggunakan isolat jamur entomopatogen sebagai bioinsektisida. Penelitian ini diawali dengan isolasi jamur entomopatogen menggunakan metode moist chamber dengan larva M. domestica sebagai serangga pancingan. Jamur yang tumbuh pada larva dikultur dan dimurnikan pada media PDA lalu diidentifikasi. Identifikasi dilakukan melalui pengamatan makroskopis meliputi warna dan diameter koloni dan pengamatan mikroskopis meliputi struktur konidia, konidiofor, hifa, vesikula, fialid, dan sel kaki. Hasil isolasi dan identifikasi diperoleh lima jenis isolat yaitu Aspergillus sp. 1, Aspergillus sp. 2, Geotrichum sp., Penicillium sp., dan Aspergillus sp. 3.
THE EFFECT OF MAGNETIC FIELD ON ANTIBIOTIC INHIBITION FOR Escherichia coli AND Bacillus sp. Sumardi, Sumardi; Agustrina, Rochmah; Ekowati, Christina Nugroho; Putra, Rizani Oktanisyah; Hartono, Madi
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.8051

Abstract

This study was aimed to test the growth of bacteria Escherichia coli (E. coli) and Bacillus sp. which were exposed to magnetic fields. In the first stage of the study, the effect of magnetic fields on the growth of E. coli and Bacillus sp were observed. The futher study was aimed to evaluate the effect of magnetic field on antibiotic sensitivity againts the growth of E. coli and Bacillus sp. The magnetic fields treatments were 0.0 mT (control), 0.1 mT, 0.2 mT, 0.3 mT which were exposed for 10 hours for each treatment. Five antibiotics (trimethoprim, ampicillin, nalidixic acid, streptomycin and chloramphenicol) were used for each bacteria. The result showed that the magnetic field did not influence the colony growth of E.coli, but in Bacillus sp. was seen the increasing of colony area in magnetic field of 0.1 mT and 0.2 mT compared with control treatment. Antibiotic of trimethoprim, nalidixic acid, and ampicillin increase the growth inhibition of E. coli when the bacteria have been exposed to 0.1 mT; 0.2 mT; 0.3 mT magnetic field for 10 minutes. The inhibition by streptomycin and chloramphenicol antibiotic on E. coli did not affected by magnetic field exposure. The inhibition of Bacillus sp by trimethoprim and ampicillin increased when the bacteria have been exposed to 0.2 mT and 0.3 mT magnetic field for 10 minutes. The inhibition of nalidixic acid, streptomycin and chloramphenicol to Bacillus sp. did not affected by the magnetic field exposure.
DETEKSI KRISTAL PROTEIN PADA ISOLAT BACILLUS SP. DENGAN PEWARNAAN COOMASSIE BRILLIANT BLUE Siallagan, Maria Denada; Ekowati, Christina Nugroho; Sumardi, Sumardi; Rosa, Emantis
Biospecies Vol. 13 No. 2 (2020): Juli 2020
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v13i2.9562

Abstract

Bacillus is one of the genera of bacteria that has the potential to be developed in the field of biotechnology. Bacillus is able to produce protein crystals during the stationary phase in its life cycle. This protein crystal is a protoxin that is active will be pathogenic in insects. In this study, protein crystal staining was carried out using coomassie brilliant blue dye on the isolate of Bacillus sp. with isolate code B1, B2, and B3. Before being stained, the three isolates were bred on Nutrient Broth (NB) medium at pH 5, pH 7, and pH 9, then incubated for 72 hours. Based on research results, the three isolates were detected to produce protein crystals. Crystal protein of Bacillus sp. on media with a pH of 5 and pH 7 located at the end of the spore, while the protein crystal of Bacillus sp. isolate on media with a pH of 9 located on the outside of the spores.
Pertumbuhan Ipomoea reptans Poir. dengan Aplikasi The Kompos Serat Bromelain Terinduksi Trichoderma sp. dan Aspergillus sp. Firda Safira, Masnoni; Irawan, Bambang; Ekowati, Christina Nugroho; Agustrina, Rochmah
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.169

Abstract

Bromelain fiber is the product of extracting the enzyme bromelain from pineapple hulls or peels. Bromelain fiber contains lignocellulose, which, when fully decomposed, is very beneficial for the environment, but it is quite challenging to degrade naturally. Hence, it requires a decomposer agent. The decomposer agents used in this study were cellulolytic fungi Aspergillus sp. (Bioggp 3) and ligninolytic Trichoderma sp. (Bioggp 2). This study aims to determine the effect of compost tea (CT) bromelain fiber induced by these fungi and obtain the best dose of the CT on the growth of land kale (Ipomoea reptans Poir.). This research was conducted in April–July 2022 at the Microbiology Laboratory of FMIPA Unila. This study used a completely randomized design (CRD) with 7 treatments and 4 replications, namely P0 (control), P1 (ACT 25%), P2 (NACT 25%), P3 (ACT 50%), P4 (NACT 50%), P5 (ACT 75%), P5 (NACT 75%). The parameters measured were plant height and number of leaves. The data were analyzed using ANOVA α = 5% followed by the Least Significant Difference (LSD) test with a 5% confidence interval. This study showed that the results with the highest height value and number of leaves were found in P3 (ACT 50%) while the lowest was in P0 (water control). It can be concluded that the ACT and NACT significantly affect the height and number of leaves, and the best dose was found in ACT 50% starting from day 10, 15 , 20, and 25 after planting. Keywords: Aspergillus sp., bromelain fiber, compost tea, Ipomoea reptans Poir, Trichoderma sp.
Co-Authors Arifiyanto, Achmad Bambang Irawan Edelina Sinaga Sinaga, Edelina Sinaga Farisi, Salman Farisi, Salman Firda Safira, Masnoni Hairisah, Suminta Frida Madi Hartono Ningsih, Agung Setia Putra, Rizani Oktanisyah Qatrunada, Vanya Riska Damayanti, Riska Rochmah Agustrina Rosa, Emantis Rudy Sutrisna Septiana, Nofita Siallagan, Maria Denada Sumard, Sumard Sumardi .