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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences International Journal of Education, Management, and Technology
Aisami, Abubakar
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Agriculture, Biological Sciences & Forestry Computer Science & IT Education Electrical & Electronics Engineering Engineering Environmental Science Materials Science & Nanotechnology

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Optimization of Cultural and Physical Parameters for Phenol Biodegradation by Newly Identified Pseudomonas sp. AQ5-04 Aisami, Abubakar; Yasid, Nur Adela; Shukor, Mohd Yunus Abd
Journal of Tropical Life Science Vol 10, No 3 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.03.06

Abstract

Phenol is widely used by many industries and it is one of  the highly toxicenviron-mental pollutants. Bioremoval is one of the most effective methods to remove phenol compared to other physio-chemical methods. Identification was carried out using 16s rRNA sequencing. Mineral salt media with 0.5 g/L phenol as the sole source of carbon. Factors influencing phenol degradation were optimised via one-factor-at-a-time and response surface methodology. Optimum degradation was achieved at pH 7.5, the temperature of 30°C and ammonium sulphate at 0.4 g/L. Using Response surface methodology the incubation period was reduced to 36 h compared to the OFAT approach where it takes 72 hours. The effect of 10 heavy metals at various concentrations was tested. The optimum values used for tempe-rature, pH, ammonium sulphate and salinity for both the OFAT and RSM have correlated with the only pH displayed the slighted difference of 7.0 for OFAT and 7.5 for RSM. This shows the closest optimum conditions for both methods. The strain is also resistance to some heavy metals usually found in polluted environ-ments together with phenol. Therefore, it can be clearly stated that Pseudomonas sp. strain AQ5-04 is the potential candidate for phenol bioremediation and further studies in the field of bioremediation. The bacterium can degrade phenol in the presence of  between 1 to 3 ppm of the heavy metals As, Cd, Co, and Zn while growth and degradation were inhibited by Hg, Ag, Cu and Ni at 1 ppm. The isolate is a potential strain for further bioremediation studies.
Biodegradation of Plastic and the Role of Microbial Enzymes in Plastic Waste Management Hammari, Abubakar Muhammad; Aisami, Abubakar; Ibrahim, Maryam; Jauro, Muhammad Jauro; Bashir, Usman Adamu
International Journal of Education, Management, and Technology Vol 4 No 1 (2026): International Journal of Education, Management, and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ijemt.v4i1.8198

Abstract

Plastic pollution has emerged as a major environmental problem affecting terrestrial and aquatic ecosystems and posing human health risks through microplastic exposure and chemical leaching. This research reviews the biodegradability of common plastics and examines biological approaches—particularly microbial and enzymatic degradation—as complementary strategies to mechanical and chemical recycling. Drawing on recent experimental and review literature, it describes microbial colonization of plastic surfaces and the enzymatic mechanisms underlying depolymerization, with emphasis on PET hydrolases such as PETase and MHETase, cutinases, and oxidative enzymes including laccases. The study further outlines analytical methods for assessing plastic degradation and evaluates strategies to enhance enzymatic depolymerization, such as protein engineering, enzyme immobilization, and physical or chemical pretreatment of substrates. In addition, it discusses scale-up challenges, biosafety considerations, and pathways for integrating biotechnological solutions into circular economy frameworks, and formulates recommendations for future research and pilot-scale deployment. Overall, the review highlights the potential and limitations of microbial and enzymatic plastic biodegradation as part of a broader portfolio of interventions needed to mitigate plastic pollution.
Co-Authors Bashir, Usman Adamu Hammari, Abubakar Muhammad Ibrahim, Maryam Jauro, Muhammad Jauro Shukor, Mohd Yunus Abd Yasid, Nur Adela