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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner
Santoso, Ferdinand Prayogo Cahyo
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Spike Glycoprotein 1 Partial Gene Analysis of GI-19 (QX-like) Infectious Bronchitis Virus Isolated and Propagated from Breeder, Broiler, and Layer Chickens in Java Region Santoso, Ferdinand Prayogo Cahyo; Widayanti, Rini; Widyarini, Sitarina; Wibowo, Michael Haryadi
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.414-424

Abstract

This study aims to identify and characterize receptor binding sites (RBS) and antigenic sites (HVR-I and II) of the S1 gene fragment of the infectious bronchitis virus (IBV) isolated and propagated from commercial chickens in Java Region to monitor recent circulating virus. The samples in this study were the organs which indicated infectious bronchitis infection. The stages of this research consisted of making virus suspensions, isolation, and propagation, as well as molecular detection and characterization of viruses. Virus isolation and propagation were carried out on chicken embryonated eggs aged 11 days via the allantoic route. Culture confirmation was performed by RT-PCR of the S1 gene fragment, followed by sequencing and bioinformatics analysis. The 168-hour propagation was observed in both dwarfed and curled embryos of two isolates from 11 isolates detected as IBV-positive. Phylogenetic tree construction resulted in all isolates being grouped as GI-19 genotype (QX-like). Amino acid identity among QX-like strains was calculated at 87–100%. A total of 210 predicted amino acid residues were observed, including 31 substitutions and 2 deletions. Conclusions of this study were identified and characterized as GI-19 genotype (QX-like) IBV with amino acid changes on S1 fragment from breeder, broiler, and layer chickens in Java Region.
Detection of Newcastle Disease, Avian Influenza, dan Infectious Bronchitis Virus from Commercial with Respiratory Clinical Signs Santoso, Ferdinand Prayogo Cahyo; Wahyuni, Agnesia Endang Tri Hastuti; Untari, Tri; Asmara, Widya; Wibowo, Michael Haryadi
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.93221

Abstract

Clinical symptoms in the chicken’s respiratory system, such as sores, nasal discharge, gasping, and so on, can be caused by a variety of possible socks. These signs may be caused by a virus, usually followed by economic losses. Causative diagnosis, especially virus detection, is needed to establish the cause of the symptoms. The study aims to detect Newcastle Disease (ND), Avian Influenza (AI), and Infectious Bronchitis (IB) cases of respiratory symptoms in commercial chickens by 2023. The samples studied in this study were organs from commercial chicken broilers and layers with respiratory symptoms. The organ was prepared as a suspension, then centrifuged to take out the supernatants. Supernatants were extracted using commercial extraction kits to produce templates for molecular detection. Molecular detection was performed using the RT-PCR two-step method. Primer pairs were used for the detection of ND, AI subtypes H5 and H9, as well as IB. The results were visualized by electrophoresis with 1.5% agarose. Interpretation of the positive result of RT-PCR based on the appearance and length of amplicons compared with the positive control of each virus. Positive RT-PCR samples with thick amplicon quality were subjected to sequencing and bioinformatics analysis. Results of detection with RT-PCR against 41 samples are positive ND of 1 out of 8 samples (12.5%), AI of 2 out of 17 samples (11.7%), and IB of 5 out of 16 samples (31.3%). Based on the molecular detection with RT-PCR, the symptoms of respiration in commercial chickens are confirmed due to the cause, namely the virus: ND genotype VII-i, AI subtype H5 clade 2.3.2, AI subtype H9, and IB genotype GI-19 (QX-like).
Co-Authors Agnesia Endang Tri Hastuti Wahyuni Michael Haryadi Wibowo Rini Widayanti Sitarina Widyarini Tri Untari Widya Asmara