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All Journal The Journal of Experimental Life Sciences (JELS) Biotropika
Putranto, Septiawan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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The Effect of a Combination of Marsilea crenata and Moringa oleifera Leaves Ethanol Extract on Follicles and Oocytes Diameter of Goat: In Vitro Study Putranto, Septiawan; Widhaningrum, Siska Nanda; Rahayu, Sri; Ciptadi, Gatot
Biotropika: Journal of Tropical Biology Vol. 11 No. 2 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.02.05

Abstract

Marsilea crenata and Moringa oleifera are herbal plants widely used in various alternative medicines due to their antioxidants and estrogenic activity. The current study investigated the effect of a combination M. crenata and M. oleifera leaves ethanol extract on the growth of follicles and oocytes of goats in vitro. Twenty-four antral follicles of Ettawa Crossbred Goat (Capra aegagrus hircus) were randomly grouped into four groups: K (control, without extract treatment), D1 (21.6 microgram/mL of M. crenata ethanol extract), D2 (21.6 microgram/mL of M. oleifera leaves ethanol extract), and D3 (combination of M. crenata and M. oleifera leaves ethanol extract at the dose of 21.6 microgram/mL). The follicle diameter was measured on days 0, 3, and 6 of culture, while the oocyte diameter was only examined on day 6. After being treated with the combination of M. crenata and M. oleifera leaves ethanol extract (D3), the diameter of the ovarian follicles increased significantly (p<0.05) from day-0 to day-6. D3 group also greatly enhanced estrogen levels in the follicular fluid of goats compared to a single extract. However, the diameter of the oocyte was not significantly increased after being treated with a single or combination of both extracts. Therefore, this study suggested that combination of M. crenata and M. oleifera leaves ethanol extract can be used as phytoestrogen to enhance follicular growth and estrogen levels.
Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study Widhaningrum, Siska Nanda; Putranto, Septiawan; Rahayu, Sri; Ciptadi, Gatot
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.06

Abstract

Water clover (Marsilea crenata) is one of the herbal plants that has been using in alternative medicine. It possesses pharmacologically active compounds like flavonoid, which has cellular activities such as antioxidant and estrogenic activity. This study aimed to evaluate the impact of water clover ethanol extract (WCE) at different concentrations on the growth of follicles and oocytes based on follicles and oocytes diameter, respectively, after six days of culture. This experimental study used 24 isolated antral follicles (2.5-3.2 mm), which were randomly divided into four groups including control (without supplemented WCE) and experimental groups that supplemented with different concentrations of WCE (21.6, 43.2, and 86.4 µg ml-1) in culture medium for six days culture. The diameter of follicles was measured on days 0, 3, and 6. Additionally, oocytes diameter was also measured on day 6. The results indicate that the mean diameter of antral follicles and oocyte diameter of WCE 43.2 µg ml-1 was significantly increase compared to the other groups (P≤0.05). According to our results, WCE exerts its effect on the growth of the antral follicle and oocyte based on follicles and oocytes diameter respectively in a dose-dependent manner after six days of the antral follicle cultured.  Keywords: antral follicle, flavonoid, in vitro, oocyte, water clover.
Co-Authors Gatot Ciptadi Siska Nanda Widhaningrum SRI RAHAYU