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All Journal Jurnal Medika Cendikia (e-Journal)
Apriyadi, Yadi
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Nursing Public Health

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UTILIZATION CRYSTAL VIOLET AS NUCLEI DYE IN HISTOPATHOLOGY OF BREAST CANCER Mamay, Mamay; Apriyadi, Yadi; Nurisani, Astari; Hardianti, Dian
Jurnal Medika Cendikia Vol 10 No 2 (2023): Jurnal Medika Cendikia
Publisher : Karsa Husada Health Institute Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/jmc.v10i2.226

Abstract

Microscopic examination of cancer development can be identified through histopathological examination. Hematoxylin–eosin (HE) is the gold standard for staining method. This processing can be obstructed if hematoxylin reagent out of stock in the laboratory. Other reagents are needed that can replace the use of hematoxylin, one of which is crystal violet. This study aims to evaluate the use of crystal violet as a nuclei stain in breast cancer preparations. The research sample consisted of 15 breast cancer preparations taken at the sample bank in the anatomical pathology laboratory of RSUD Dr. Slamet Garut. The results showed that from 15 preparations, it was observed that 4 preparations (27%) stained worth and 11 preparations (73%) stained poorly. Based on the research results, crystal violet crystal violet has a low core coloring ability in breast cancer preparations. From the results of this study, it is recommended to modify the crystal violet reagent by paying attention to the acidity of pH and concentration of crystal violet.
Co-Authors Astari Nurisani Dian Hardianti, Dian Mamay, Mamay