Article Per Year (5 Year)
p-Index From 2020 - 2025
1.325
P-Index
This Author published in this journals
All Journal Journal of Midwifery and Nursing INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Medika Cendikia (e-Journal) Medicra (Journal of Medical Laboratory Science/Technology) Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia
Mamay, Mamay
Unknown Affiliation
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Nursing Public Health Social Sciences Other

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Utilization ethanol extract of sappan wood as an alternative in escherichia coli staining mamay, mamay
Journal of Midwifery and Nursing Vol. 6 No. 1 (2024): January: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v6i1.4565

Abstract

In life, sappan wood is used as an ingredient in traditional drinks that has antioxidant, antimicrobial and has stable pigments. The content of red pigment, brazilin in sappan wood can be used as a dye. The purpose of this study was to determine the potential of sappan wood ethanol extract as an alternative to safranin in gram staining. Ethanol extract of sappan wood 6% was added with alum mordant to obtain a red pigment. The bacteria used Eschericia col, isolated from water. Staining using ethanol extract of sappan wood was carried out for 30 seconds, 3, 5 and 10 minutes. This stain was compared with safranin. The results showed that staining using sappan wood extract shows clarity of the visual field, shape and good color. Based on the results, the ethanol extract of sappan wood with mordant can be used as an alternative to safranin in the gram-staining.
Minimal inhibitory concentration (MIC) of sodium hypochlorit against bacterial isolate from equipment in the microbiology laboratory Mamay, Mamay; Mar’atiningsih, Lia; Atoilah, Elang M.; Mutia, Sifa Rinda
Journal of Midwifery and Nursing Vol. 6 No. 2 (2024): May: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v6i2.5171

Abstract

Microbiology laboratory equipment is susceptible to bacterial contamination and therefore has the potential to become a medium for the spread of disease and infection. The existence of a less than sterile microbiological analysis process in the STIKes Karsa Husada Garut Microbiology Laboratory causes bacterial contamination of laboratory equipment such as bacterial analysis that does not comply with standards or the use of less than sterile equipment. To control the spread of contaminants, a decontamination process is required using a chemical disinfectant, namely sodium hypochlorite. Measuring the effectiveness of a disinfectant by looking at or can be determined by the Minimum Inhibitory Concentration (MIC). The aim of this research was to determine the effectiveness of sodium hypochlorite disinfectant in inhibiting the growth of microorganisms in bacterial isolates from the STIKes Karsa Husada Garut Microbiology Laboratory equipment. The data analysis used was analyzed descriptively. The results of this research are the Minimum Inhibitory Concentration (MIC) of sodium hypochlorite disinfectant on bacterial isolates from the STIKes Karsa Husada Garut Microbiology Laboratory equipment at a concentration of 4%. So it can be concluded that sodium hypochlorite disinfectant with a concentration of 4% is effective in inhibiting the growth of microorganisms.
Utilization Dishwashing Soap as a Subtitute of Xylol in the Deparaffinization process of Hematoxylin-Eosin Dye: Review Article mamay, Mamay; Mutmaina, Gina Nafsa; Nurahma, Ina Aflaha
Medicra (Journal of Medical Laboratory Science/Technology) Vol 5 No 1 (2022): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i1.1629

Abstract

Deparaffinization in the hematoxylin eosin staining process aims to remove paraffin from the tissue, clean the tissue and maximize dye absorption. Utilization xylol as deparaffinization has a drawback, if inhaled too much it will cause harm to the health of the laboratory staff. Considering this, a safer alternative to xylol is needed, one of which is dish washing soap. This study was conducted to determine the use of liquid dish soap at the deparaffinization stage on the quality of tissue staining results. The research method in this research is a literature review study conducted based on sources from several articles published digitally in the Science Direct, Pub Med, NCBI, Researchgate, Google Scholar with the keywords deparafinization, xylene, dish washing soap and Hematoxylin-eosin. Based on the results of a review of ten journals, the concentration of dishwashing soap used was 1.5%, 1.7%, 2% and 2.5% with the most widely used concentration of 1.7%. The most used deparaffinization time with dish soap was 1 minute, one study used 2 minutes. Of the several differences in deparaffinization time and concentration of dish soap, the results adequacy of nuclear and cytoplasmic staining, clarity, uniformity and crispness of staining were very good in almost all studies, there was no difference in quality with the use of xylol. So it can be concluded that the use of dish soap in the deparaffinization process for Hematoxylin staining showed good staining of all tissues. Dish soap can be used as an alternative agent in deparaffinization of Hematoxylin-Eosin staining.
Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining Mamay, Mamay; Ernawati, Ernawati; Nurisani, Astari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.3961

Abstract

Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology.
PENYULUHAN KADAR CRP (C-REACTIVE PROTEIN) PADA PENDERITA TUBERKULOSIS DI KECAMATAN CILAWU SEBAGAI PENANDA ADANYA INFLAMASI Nurisani, Astari; Mamay, Mamay; Mutmaina, Gina Nafsa; Sugiah, Sugiah; Pratama, Wisnu Arya
Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Vol. 2 No. 2 (2023): Ekalaya Journal
Publisher : Nindikayla Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57254/eka.v2i2.57

Abstract

Infection due to tuberculosis bacteria will cause inflammation in the body which will stimulate the emergence of C-Reactive Protein as a biomarker of inflammation. C-Reactive Protein (CRP) is a polypeptide molecule of the pentraxin group which is produced in the liver and controlled by cytokines especially Interleukin-6 (IL-6). CRP is useful for monitoring disease progression. CRP concentration is related to disease severity, infection and treatment effect. The purpose of this community service is to see CRP (C-Reactive Protein) levels in tuberculosis sufferers in the Cilawu sub-district and provide an understanding of CRP examination. The method used is counseling with LCD and examination of CRP levels in tuberculosis patients. The sufferers are collected in a house that has been mutually agreed upon. The results of the activity were that most of those present had CRP examination results on the 30 samples that had been examined, 24 people (80%) had positive results, and 6 people (20%) had negative results. CRP levels in positive tuberculosis patients obtained various results between 6 mg/L to 192 mg/L. Conclusion: the results of this community service illustrate that there is an inflammatory response of CRP to tuberculosis bacterial infection. After carrying out counseling and examining CRP levels, the community, especially tuberculosis sufferers, became aware and interested in carrying out CRP examinations as a form of monitoring the level of infection they were suffering from
Antibacterial Activity of Ecoenzyme from Garut Orange Peel (Citrus nobilis var.chrysocarpha) against Propionibacterium acne Nurani Ai Erlinawati, Nurani Ai Erlinawati; Mamay, Mamay; Yogi Rahman Nugraha, Yogi Rahman Nugraha
Jurnal Medika Cendikia Vol 12 No 01 (2025): Jurnal Medika Cendikia
Publisher : Karsa Husada Health Institute Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/jmc.v12i01.323

Abstract

Acne is a skin disorder that often occurs in adolescents. Propionibacterium acnes is a bacteria that causes acne. The use of antibacterial substances is very important to prevent and control this bacterial infection. One of the advances in the use of antibacterials comes from the use of ecoenzyme. The process of managing organic waste with ecoenzyme can be an effective and environmentally friendly waste management strategy. Garut orange with the scientific name Citrus nobilis var. chrysocarpa is a local wisdom fruit. Orange peel which is a waste that increases in quantity when the harvest season arrives can be used in the manufacture of ecoenzyme. This study aims to evaluate the antibacterial ability of ecoenzyme from garut orange peel against Propionibacterium acnes bacteria. The fermentation time for ecoenzyme production varied from 30, 60 and 90 days.. The method for the eco enzyme antibacterial test uses the disc diffusion method. Three variations in eco enzyme concentration used to inhibit the growth of isolated bacteria are 33%, 66% and 100%. Ecoenzyme from Garut orange peel shows antibacterial activity against Propionibacterium acne. Antibacterial activity shows an increase with increasing fermentation time of ecoenzyme
UTILIZATION CRYSTAL VIOLET AS NUCLEI DYE IN HISTOPATHOLOGY OF BREAST CANCER Mamay, Mamay; Apriyadi, Yadi; Nurisani, Astari; Hardianti, Dian
Jurnal Medika Cendikia Vol 10 No 2 (2023): Jurnal Medika Cendikia
Publisher : Karsa Husada Health Institute Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/jmc.v10i2.226

Abstract

Microscopic examination of cancer development can be identified through histopathological examination. Hematoxylin–eosin (HE) is the gold standard for staining method. This processing can be obstructed if hematoxylin reagent out of stock in the laboratory. Other reagents are needed that can replace the use of hematoxylin, one of which is crystal violet. This study aims to evaluate the use of crystal violet as a nuclei stain in breast cancer preparations. The research sample consisted of 15 breast cancer preparations taken at the sample bank in the anatomical pathology laboratory of RSUD Dr. Slamet Garut. The results showed that from 15 preparations, it was observed that 4 preparations (27%) stained worth and 11 preparations (73%) stained poorly. Based on the research results, crystal violet crystal violet has a low core coloring ability in breast cancer preparations. From the results of this study, it is recommended to modify the crystal violet reagent by paying attention to the acidity of pH and concentration of crystal violet.
Co-Authors Apriyadi, Yadi Astari Nurisani Atoilah, Elang M. Dian Hardianti, Dian Ernawati Ernawati Mar’atiningsih, Lia Mutia, Sifa Rinda Mutmaina, Gina Nafsa Nurahma, Ina Aflaha Nurani Ai Erlinawati, Nurani Ai Erlinawati Pratama, Wisnu Arya Sugiah, Sugiah Yogi Rahman Nugraha