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All Journal Journal of Midwifery and Nursing INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Medika Cendikia (e-Journal) Science Midwifery Jurnal Kesehatan Jurnal Pengabdian Masyarakat DEDIKASI Jurnal Pengabdian Masyarakat Sains dan Teknologi Safari : Jurnal Pengabdian Masyarakat Indonesia Jurnal Kesehatan Amanah Jurnal Sains dan Kesehatan Student Scientific Creativity Journal Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Usada Nusantara: Jurnal Kesehatan Tradisional Jurnal Abdimas Indonesia Aspirasi : Publikasi Hasil Pengabdian dan Kegiatan Masyarakat Jurnal Riset Ilmu Kesehatan Umum dan Farmasi Jurnal Pengabdian Bidang Kesehatan Compromise Journal : Community Proffesional Jurnal Siti Rufaidah
Astari Nurisani
Department Of Medical Laboratory Technology, STIKes Karsa Husada, Garut, Indonesia
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Magnesium and insulin leaf combination on glucose, low-density lipoprotein and malondialdehyde levels in wistar rats with diabetes mellitus Astari Nurisani; Mamay Mamay; Ana Hidayati Mukaromah; Purwanto AP
Science Midwifery Vol 11 No 1 (2023): April: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i1.1226

Abstract

Diabetes mellitus is a metabolic disease characterized by chronic hyperglycemia due to impaired insulin secretion, insulin receptor sensitivity and/or both. Hyperglycemia can trigger oxidative stress conditions that can increase the risk of micro and macrovascular complications. Preventive efforts need to be done such as giving supplements and traditional therapies that have low side effects, namely magnesium and insulin leaves. This research is experimental type with post test only control group. The samples in this study were 30 male wistar rats weighing 170-300g which had healthy conditions adapted for 7 days, given standard feed and given ad libitum drinking water. The results showed that the glucose level in the treatment group 3 when compared to the negative control group had a percentage decrease of 26.49%. LDL and MDA levels were lower in the combination treatment group of magnesium and insulin leaves when compared to the negative control group. The ANOVA results showed that there was a significant difference between the groups in the glucose test results (p < 0.05), and there was no significant difference between the groups in the LDL and MDA results (p > 0.05). The conclusion of this study was that the best results when compared to negative controls were the combination of magnesium at a dose of 300 mg and insulin leaves at a dose of 300 mg/KgBB had a decreased percentage of LDL levels by 52.38% and MDA levels of 29.11%, while for glucose levels the combination of magnesium doses 300 mg and insulin leaves 75 mg/KgBW had a percentage reduction of 26.49%
Isolation and identification of escherichia coli in well water located in Garut Regency Sugiah Sugiah; Gina Nafsa Mutmaina; Mamay Mamay; Astari Nurisani
Science Midwifery Vol 11 No 1 (2023): April: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i1.1236

Abstract

Water is one of the most important needs for daily life, especially well water. In the Garut district area well water is used for daily activities ranging from washing, drinking, bathing, ablution to being used for drinking water consumption. This study aims to isolate and identify well water from 10 villages located in the Garut Regency. Sampling was done using purposive sampling method. The test was followed by a presumptive test using Lactose Broth (LB), a reinforcing test using 2% BGLB media and a complementary test consisting of EMB agar media and biochemical tests. The results showed that of the 10 well water samples, 7 met the requirements of proper water quality and 3 did not meet the requirements of clean water with MPN values from three villages, namely Kampung Tanjung kamuning 200/ml, Kampung salaawi 241/100ml and Kampung Cipepe 241/100ml. EMB agar media is characterized by the formation of a metallic green color with black spots colonies and biochemical tests that show the well water has been contaminated with Escherichia coli bacteria.
Pemeriksaan C-Reactive Protein (CRP) Kualitatif dan Semi Kuantitatif pada Penderita Tuberkulosis Astari Nurisani; Mamay Mamay; Meti Rizki Utari; Zahara Farhan; Wisnu Arya Pratama
Jurnal Kesehatan Vol. 10 No. 2 (2022): Oktober: Health Journal "Love That Renews"
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55912/jks.v10i2.99

Abstract

Infeksi akibat bakteri tuberkulosis akan mengakibatkan peradangan didalam tubuh yang akan merangsang munculnya C-Reactive Protein sebagai biomarker peradangan. C-Reactive Protein (CRP) merupakan molekul polipeptida kelompok pentraxin yang diproduksi di hati dan dikontrol oleh sitokin khususnya Interleukin-6 (IL-6). CRP bermanfaat untuk memonitor perkembangan penyakit. Konsentrasi CRP berhubungan dengan tingkat keparahan penyakit,infeksi dan efek pengobatan. Tujuan penelitian ini adalah untuk melihat gambaran kadar CRP (C-Reactive Protein) pada penderita tuberkulosis di RSUD Dr. Slamet Garut. Pemeriksaan CRP dilakukan secara kualitatif dan semi kuantitatif. Hasil pemeriksaan CRP pada 30 sampel yang telah diperiksa didapat hasil positif sebanyak 24 orang (80%), dan negatif sebanyak 6 orang (20%). Berdasarkan hasil pemeriksaan diperoleh kadar CRP pada pasien tuberkulosis positif didapat hasil beragam antara 6 mg/L sampai 192 mg/L. Hasil penelitian ini menggambarkan adanya respon inflamsi CRP terhadap infeksi bakteri tuberkulosis. Saran dalam penelitian ini adalah data penelitian ini digunakan sebagai data acuan penelitian selanjutnya dalam pemeriksaan CRP metode kuantitatif mengenai gambaran kadar CRP sebelum dan sesudah pengobatan..
Overview of Triglyceride levels in Lipemic Serum using Polyethylene Glycol (PEG) 6000 Sugiah Sugiah; Kurniawan Dewi Budiarti; Astari Nurisani; Gina Nafsa Mutmaina; Alya NSY
Journal of Midwifery and Nursing Vol. 5 No. 3 (2023): September: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v5i3.4187

Abstract

Lipemic causes interference with wavelength and light scattering caused by the presence of lipid particles. This study aims to determine the description of triglyceride levels in lipemic serum with and without the addition of polyethylene glycol (PEG) 6000. This type of research is experimental. The research conducted was descriptive research. The samples used were lipemic serum totaling 30 samples with triglyceride levels above 300 mg/dl. The results of the data analysis showed a mild lipemic level of 22 samples with an average of 379.00 mg/dl, a moderate lipemic level of 5 samples with an average of 558.40 mg/dl, and a severe lipemic serum of 3 samples with an average of 943.40 mg/dl. Based on the results of triglyceride levels without the addition of PEG 6000, the average level is 465.33 mg/dl, while the results of triglyceride levels using PEG 6000, the average level is 243.23 mg/dl. Based on the difference in triglyceride levels before and after adding PEG 6000, namely at the mild level, the average is 215.00 mg/dl (56%), at the moderate level, the average is 261.00 mg/dl (46%), and at the severe level, the average is 212.30 mg/dl (22%). It can be concluded that there are differences in triglyceride levels in lipemic serum before and after adding PEG 6000.
Perbedaan Kadar Glukosa Darah Sewaktu Pada Sampel Yang Langsung Diperiksa Dan Yang Ditangguhkan Selama 2 Jam Pada Suhu 2-8°C Sugiah Sugiah; Gina Nafsa Mutmaina; Astari Nurisani
USADA NUSANTARA : Jurnal Kesehatan Tradisional Vol. 1 No. 1 (2023): Januari: USADA NUSANTARA
Publisher : Institut Nalanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.583 KB) | DOI: 10.47861/usd.v1i1.126

Abstract

Pelayanan laboratorium klinik merupakan bagian terpenting dari pelayanan yang diperlukan untuk menegakan diagnosis.Pemeriksaan yang sering dilakukan adalah pemeriksaan glukosa dengan spesimen yang banyak digunakan adalah serum. Hal ini dikarenakan kadar glukosa dalam serum yang lebih stabil. Jika terjadi penundaan spesimen pada pemeriksaan glukosa maka akan terjadi glikolisis yang dapat menyebabkan kadar glukosa dalam serum berkurang 10 mg/dl per jam. Penelitian ini bertujuan untuk mengetahui adanya perbedaankadar glukosa darah sewaktu pada sampel yang langsung diperiksa dan yang ditangguhkan selama 2 jam pada suhu 2-8°c. Penelitian ini menggunakan metode GOD – PAP (Glucose Oxsidase – Peroxidase Aminoantypirin)dengan jumlah spesimen sebanyak 43 sampel. Hasil pemeriksaan glukosa dengan serum yang segera diperiksa memiliki rata-rata 120,8 mg/dL sedangkan hasil pemeriksaan glukosa yang disimpan selama 2 jam pada suhu 2-8°c memiliki rata-rata 113,4 mg/dL. Adanya perbedaan antara hasil pemeriksaan glukosa dengan serum yang langsung diperiksa dan disimpan selama 2 jam pada suhu 2-8°c. Maka dari itu, bagi tenaga kerja laboratorium disarankan agar tidak menunda spesimen agar hasil pemeriksaan maksimal dan akurat sesuai dengan keadaan pasien.
Gambaran Efektivitas Tepung Biji Nangka (Artocarpus heterophyllus) sebagai Media Alternatif Pertumbuhan Jamur Aspergillus niger Lia Mar’atiningsih; Sugiah Sugiah; Gina Nafsa Mutmaina; Mamay Mamay; Astari Nurisani; Muhammad Hadi Sulhan; Meti Rizki Utari; N.Ai Erlinawati; Noer Halisa Wardah
Jurnal Sains dan Kesehatan Vol. 8 No. 1 (2024): Juni : Jurnal Sains dan Kesehatan (JUSIKA)
Publisher : Universitas Muhamadiyah Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57214/jusika.v8i1.541

Abstract

The culture medium functions to provide nutrition and as a place for microorganisms to grow optimally. The price of agar media is expensive and quite difficult to reach, making examination of microorganisms in the laboratory expensive. This can be overcome by using alternative media that are easy to obtain. One example of a natural ingredient that can be used as an alternative medium is jackfruit seeds which are then processed into flour. Jackfruit seeds can be used as an alternative ingredient because they contain nutrients, namely carbohydrates. The aim of this research was to see the effectiveness of jackfruit seed flour for the growth of the Aspergillus niger fungus. The type of research used is descriptive by describing the growth of the Aspergillus niger fungus on alternative agar media, namely jackfruit seed flour. . After data collection, the data is then processed using a tabulating stage which is presented in table form by showing the growth of the Aspergillus niger fungus macroscopically and microscopically using 10x and 40x magnification which is then grouped based on positive and negative categories.
Upaya Pencegahan Hasil Positif Palsu melalui Edukasi Pemeriksaan Narkotika pada Siswa Sekolah Mengenah Kejuruan Mamay Mamay; Lia Mar’atiningsih; Muhammad Hadi Sulhan; Astari Nurisani; Gina Nafsa Mutmaina; Meti Rizki Utari
Jurnal Pengabdian Masyarakat Sains dan Teknologi Vol. 3 No. 2 (2024): Juni : Jurnal Pengabdian Masyarakat Sains dan Teknologi
Publisher : Fakultas Teknik Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58169/jpmsaintek.v3i2.501

Abstract

Vocational High School (SMK) is a place of education that prepares its students to be ready to face the world of work. Drug tests are often carried out targeting prospective employees as a requirement to enter the workplace in an agency. Knowing about drug tests is very important for Vocational High School (SMK) students who will enter the world of work. Education about false positive results in drug tests is very important for SMK students who will enter the world of work. The right education program can increase efforts to prevent false positives in drug tests when entering the world of work for SMK graduates. To increase knowledge related to drug tests and false positive detection, health education is carried out and also a demonstration of drug tests on urine. Before and after education, each student takes a pre-test and post-test through a questionnaire to see the increase in their knowledge. After community service was carried out, with education about drug tests, there was an increase in understanding about drug tests, the drug test process with immunochromatography (ICT) and the detection of false positive results in drug tests.
Edukasi Penyakit Tuberkulosis dan Pemeriksaan Laboratorium pada Siswa SMA di Kecamatan Bayongbong Garut Mamay Mamay; Astari Nurisani; Gina Nafsa Mutmaina; Sugiah Sugiah; Muhammad Hadi Sulhan; N Ai Erlinawati
ASPIRASI : Publikasi Hasil Pengabdian dan Kegiatan Masyarakat Vol. 1 No. 6 (2023): November : ASPIRASI : Publikasi Hasil Pengabdian dan Kegiatan Masyarakat
Publisher : Asosiasi Periset Bahasa Sastra Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/aspirasi.v1i6.45

Abstract

Mycobacterium tuberculosis is a bacterium that causes the infectious disease pulmonary tuberculosis (TB). Bayongong District is ranked second in terms of TB cases found in Garut Regency. The involvement of adolescents in controlling TB disease is needed as an agent of change to increase early detection of TB in their family members. With early detection of TB disease, treatment can be started quickly and transmission can be controlled. To increase teenagers' knowledge and awareness of TB disease, it is necessary to provide education to teenagers, namely high school students in Bayongbong District, about the importance of recognizing symptoms, transmission, prevention and treatment, as well as laboratory examinations for TB disease. Education is carried out by presenting material related to TB disease. Apart from that, students were also shown a video on how to carry out a TB examination using BTA (Acid Fast Bacteria) staining and TCM (Rapid Molecular Test). Before and after education, each student received a questionnaire designed to measure their level of knowledge. After community service, education about TB disease among high school students resulted in an increased understanding of TB symptoms, transmission, prevention, treatment and examination.
Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining Mamay, Mamay; Ernawati, Ernawati; Nurisani, Astari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.3961

Abstract

Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology.
Edukasi Kualitas Air Bersih Bebas Kontaminasi Bakteri Coliform pada Masyarakat Jungsereh Garut Sugiah Sugiah; Gina Nafsa Mutmaina; Mamay Mamay; Lia Ma’artiningsih; Astari Nurisani; Muhammad Hadi Sulhan; Meti Rizki Utari
Jurnal Pengabdian Bidang Kesehatan Vol. 2 No. 2 (2024): Jurnal Pengabdian Bidang Kesehatan
Publisher : PPNI UNIMMAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57214/jpbidkes.v2i2.82

Abstract

Indonesian people still use dug wells as a source of clean water. The dug well itself has bacteriological qualities that are easily contaminated. Coliform bacteria such as Escherichia coli can cause diseases, one of which is diarrhea. Jungsereh Garut Village is an area that uses a lot of water from dug wells for daily needs such as drinking water. The aim of this outreach is to determine the quality of clean water free of coliform bacteria contamination in the Jungsereh Garut community. The target of this counseling is the community of Jungsereh Village RT.02 Kec.Mekarsari Garut, totaling 30 people. This outreach activity is a form of increasing public awareness of the quality of clean water for drinking water that is free of coliform bacteria contamination. The method of implementing this extension activity is in the form of material presentation, discussion and question and answer and filling out a questionnaire regarding the level of understanding whether there are differences before and after the intervention.
Co-Authors Aceng Ali Awaludin Alya NSY Ana Hidayati Mukaromah Apriyadi, Yadi Arbila, Meisya Arsylia Intan Zori Ichsani Dendi Leona Dian Hardianti, Dian ENGKUS KUSNADI Ernawati Ernawati Esa Risi Suazini Gina Nafsa Mutmaina Gina Nafsa Mutmaina Gina Nafsa Mutmaina Gina Nafsa Mutmainna Herlin Rusyani Kurniawan Dewi Budiarti Lia Mar’atiningsih Lia Ma’artiningsih M. Hadi Sulhan Mamay Mamay mamay Mamay, Mamay Manzilah Ziyan Meti Rizki Utari Muhamad Hadi Sulhan Muhammad Hadi Sulhan Mutmaina, Gina Nafsa Muuhammad Hadi Sulhan N Ai Erlinawati N. Ai Erlinawati N.Ai Erlinawati Noer Halisa Wardah Pratama, Wisnu Arya Purwanto AP Rismawati Rismawati Rismawati Rismawati Sandra Oktapiani Sugiah Sugiah Sugiah Sugiah Sugiah Sugiah, Sugiah Wisnu Arya Pratama Zahara Farhan