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All Journal JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis)
Pintaka Bayu Putra, Widya
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Veterinary

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Determining the FecXE Allele in Batur Ewes (Ovis aries) of Indonesia Pintaka Bayu Putra, Widya; Margawati, Endang Tri; Samsudewa, Daud
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 10, No 1 (2023): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v10i1.28654

Abstract

Batur sheep (Ovis aries) is one of the Indonesian native sheep that originated from Banjarnegara Regency. This study aimed to detect the FecXE allele of Bone Morphogenetic Protein 15 (BMP15) in Batur ewes using the sequencing method. A total of six (6) Batur ewes were randomly selected for investigation. The results showed that three (3) ewes were detected as carrier animals (FecX+/ FecXE), and the other ewes were wild-type animals (FecX+/FecX+). Unfortunately, this study did not observe a mutant animal (FecXE/FecXE). It can be concluded that the presence of the FecXE allele in Batur ewes may affect the prolificacy trait since previous studies reported that heterzygous ewes have the largest litter size traits than in wild-type ewes. Keywords: Batur sheep, BMP15 gene, litter size, sequencing,
Revealing the Genetic Mutation in a Diprosopus Monauchenos Calf Pintaka Bayu Putra, Widya; Said, Syahruddin; Febyastri, Meda; Nanditya, Wisnu Krisna; Novita, Risqa
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.005 KB) | DOI: 10.33772/jitro.v9i1.19821

Abstract

ABSTRACTDiprosopus Monauchenos (DM) is one of congenital defect that can be caused by a genetical factor. This study was conducted to reveal the genetic mutation of DM cattle. Research showed that along 354 bp of partial BTA13 sequence (69,985,393th - 69,985,745th nucleotide) in DM and normal samples were obtained by sequencing analysis. Research showed that one insertion/deletion (indel) mutation at 28th and two transition mutations at 241th and 269th positions were detected in both samples. The obtained sequence in the present study was located at the intronic region of LPIN3 gene (69,972,281th - 69,991,534th nucleotide). In the future, a depth research to investigate the genetic mutation in DM animal with whole genome analysis is important to get the results accurately.Keywords: cattle, DM, mutations, sequencing, BTA13
Co-Authors Daud Samsudewa ENDANG TRI MARGAWATI Febyastri, Meda Nanditya, Wisnu Krisna Risqa Novita, Risqa Syahruddin Said