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All Journal Jurnal Sumberdaya Hayati Journal of Health Science and Medical Therapy Biosaintifika: Journal of Biology & Biology Education
DITA, SASMIATI FARACH
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Sponge-Associated Actinobacteria: Morphological Character and Antibacterial Activity against Pathogenic Bacteria DITA, SASMIATI FARACH; BUDIARTI, SRI; LESTARI, YULIN
Jurnal Sumberdaya Hayati Vol. 3 No. 1 (2017)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.3.1.21-26

Abstract

Sponge-associated actinobacteria may diverse and have potency to produce bioactive compounds. Diversity and antimicrobial activity of indigenous sponge-associated actinobacteria isolated from the marine ecosystem in Indonesia have not much been explored. This work aimed to assess morphological and antibacterial activity of sponge-associated actinobacteria. The morphological characteristics were examined based on their color of aerial and substrate mycelia, and pigmentation, while antibacterial activities were assayed using the antagonist technique. The selected actinobacterial isolate was identified using 16S rRNA gene. Various sponge-associated actinobacteria were successfully isolated from Hyrtios sp., Callyspongia sp., and Neofibularia sp. sponges. A total of 62 actinobacterial isolates were obtained, and each isolate showed a variety of morphological characters, which could be seen in aerial mass color, substrate mass color, and pigmentation. Actinobacterial isolates were tested against human pathogenic bacteria, i.e. Staphylococcus aureus and Methicillin-Resistant S. aureus, representing Gram-positive, and Escherichia coli EPEC K1-1 and Shigella dysenteriae, representing Gram-negative. Most of actinobacterial isolates had antimicrobial activities at least against one of pathogenic bacteria. High activity was shown by NOHa.2, isolated from Neofibularia, and HRHa.5 isolated from Hyrtios. The NOHa.2 showed the highest antimicrobial activity against S. dysenteriae, meanwhile, HRHa.5 showed antimicrobial activity against 3 of 4 tested bacterial pathogens. These data showed diversity of sponge-asccociated actinobacteria from marine ecosystem in Indonesia, and several of them have potency as source of antibacterial compounds
Effectiveness of Chinese Betel Leaf Extract (Peperomia pellucida L) on the Growth of Propionibacterium acnes Bacteria Mukhlisa, Ida; Juwita ZR, Juwita ZR; Dita, Sasmiati Farach
Journal of Health Science and Medical Therapy Том 3 № 01 (2025): Journal of Health Science and Medical Therapy
Publisher : PT. Riset Press International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59653/jhsmt.v3i01.1435

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Propionibacterium acnes bacteria cause acne by producing lipase enzymes that break down free fatty acids in skin lipids. This study aimed to determine whether or not there is antibacterial activity from the extract of the Chinese betel leaf plant (Peperomia pellucida L) against Propionibacterium acnes and to determine the concentration of the inhibition zone. The study was experimental with 25%, 50%, 75% and 100% extract concentrations. The method used to test antibacterial activity in this study was the disc diffusion method, namely using discs planted on Nutrient Agar (NA) media. The maceration of Chinese betel leaves (Peperomia pellucida L) is the Chinese betel leaf extraction method. The results showed that at a concentration of 25% Chinese betel leaf extract, the results were 22.5 mm; at a concentration of 50% extract, the inhibition zone was 24 mm; at a concentration of 75% Chinese betel leaf extract, the inhibition zone was 30 mm, at a concentration of 100% Chinese betel leaf extract, the inhibition zone was 20 mm. The inhibition zone of the positive control using the antibiotic chloramphenicol was obtained at 10 mm; in the negative control using sterile distilled water, there was no inhibition zone. The results of the significance test of the Chinese betel leaf extract (Peperomia pellucida) from the Greenwood classification results in the Post hoc test show that the difference between concentrations can be stated as significant at each concentration.
Toxicity And Antioxidant Activities of Endophytic Bacteria from Butterfly Pea (Clitoria ternatea Linn.) Zahrina, Yuni; Fitri, Lenni; Ismail, Yulia Sari; Dita, Sasmiati Farach
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.13360

Abstract

Endophytic bacteria are bacteria that live in healthy plant tissues without causing damage. Several studies have reported that endophytic bacteria can produce active compounds similar to those secreted by their host and which potentially have medicinal value. Butterfly pea (Clitoria ternatea L.) was noted to be able to produce antioxidants and have toxicity potential from its compounds. Therefore, endophytic bacteria from butterfly pea have great potential to have antioxidant activity along with evaluating the toxicity level of the selected bacteria. This study aimed to determine the number of isolates, to characterize, and test the toxicity and antioxidant activities of endophytic bacteria from butterfly pea. Toxicity level was tested using the Brine Shrimp Lethality Test (BSLT) method while levels of antioxidants were tested using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. A total of fifteen endophytic bacteria were obtained and successfully purified. Based on the morphological observations, Gram staining, and biochemical test results, isolate EBT13 was determined to belong to the genus Bacillus. Isolate EBT13 was categorized as highly toxic, with the highest toxicity value with other bacterial isolates with an LC50 of 84 ppm and antioxidant activity with an IC50 value of 44.32 ppm. Based on the phylogenetic tree of 16S rRNA gene analysis, EBT13 belongs to the genus Bacillus, it forms a sister group with Bacillus pumilus with a bootstrap value of 100%. This study advances our knowledge of plant-microbe interactions by identifying a highly toxic, antioxidant-producing strain of bacteria of butterfly pea. The results have significance for the development of cytotoxic chemicals and natural antioxidants, which could advance biotechnological research and boost therapeutic purpose.
Co-Authors Juwita ZR, Juwita ZR Lenni Fitri Mukhlisa, Ida Sri Budiarti Poerwanto Yulia Sari Ismail YULIN LESTARI Zahrina, Yuni