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All Journal JBIO: jurnal biosains (the journal of biosciences) BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Biosaintifika: Journal of Biology & Biology Education
Yulia Sari Ismail
Biology Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology

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The Potential Endophytic Bacteria Isolated from Rice (Oryza sativa) as Biofertilizer Putriani, Putriani; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.252 KB) | DOI: 10.15294/biosaintifika.v11i2.18401

Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. This research aimed to obtain endophytic bacteria isolated from the root tissue of rice plants (Oryza sativa L.) which have potential to produce IAA hormones,, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Based on the results of this research, there  were six endophytic bacteria isolates obtained. They have high morphological diversity and differen ability producing IAA hormones. The highest concentration of IAA (425 ppm) was obtained  isolates from  EAP3. Isolate EAP3 also produce inhibit the growth of Xanthomonas oryzae with a 5.2 mm inhibition zone. Based on the biochemical test, EAP3 had 60% similarity with Enterobacter asburiae. Analysis of the 16S rRNA gene showed that EAP3 had the highest similarity with Enterobacter asburiae strain U4 by 99%. This research data is considered as new information about the potential of endophytic bacteria from the roots of rice plants (Oryza sativa L.) which is capable of producing IAA hormones and is able to inhibit pathogenic bacteria. This research provides information that can be used as a basis for developing endophytic bacteria as biological fertilizers. 
APPLICATION OF RICE ROOT ENDOPHYTIC BACTERIA IN CIHERANG VARIETY RICE (ORYZA SATIVA) SEEDS Fitri, Lenni; Ismail, Yulia Sari; Putriani, Putriani; Warzatullisna, Warzatullisna
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21697

Abstract

Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testing HCN production using filter paper, biochemically identification of the isolate, and applicating endophytic bacteria into rice seeds. Endophytic bacteria have different abilities in producing IAA hormone. Based on the results of the study, the highest IAA concentration (57 ppm) was obtained from KSB1 (Potassium Solubilizing Bacteria) isolates and followed by KSB3 isolate with 56 ppm. All the endophytic bacteria isolates were unable to produce HCN. Results of the study showed that KSB1 and KSB3 isolate were able to influence the growth of root length by 5.62 cm and 6.70 respectively as well as the canopy height by 8.56 cm and 8.30 cm respectively. Based on observations of colony morphology, cell morphology and biochemical tests, KSB1 was presumably belong to the genus Staphylococcus. In this study, new information about endophytic bacteria that can increase the growth of Ciherang variety rice can be obtained. This study provides information that the application of environmentally friendly bacteria is important to preserve the environment and agriculture sustainability in Indonesia without reducing the productivity of rice.
Isolation of Potential Plant Growth Promoting Rhizobacteria (PGPR) from Cassava (Manihot esculenta) Rhizosphere Soil Safriani, Suri Raihan; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25905

Abstract

Cassava is the third most important calorie source in tropical area after rice and corn. The microorganism associated with cassava roots may be potent and useful for application to promote plant growth. Plant growth promoting rhizobacteria  is a group of benefical bacteria that live in rhizosphere. The aim of this study was to isolate and to identify the potential Plant Growth Promoting Rhizobacteria (PGPR) from cassava rhizosphere soil. The study consisted of isolation and identification of bacteria based on morphological and biochemical characters, hypersensitive reaction test, the ability to solubilize potassium and phosphate, and the ability to inhibit the growth of pathogen Sclerotium rolfsii. A total of nine bacteria isolates were succesfully isolated from Cassava rhizosphere soil. Those isolates suspected as Micrococcus sp.1, Micrococcus sp.2, Micrococcus sp.3, Micrococcus sp.4, Micrococcus sp.5, Micrococcus sp.6, Neisseria sp.1, Neisseria sp.2 and Bacillus sp. All nine isolates did not show hypersensitivity reactions. Only Neisseria sp.1 and Neisseria sp. 2 were able to solubilize potassium and phosphate. All isolates were able to inhibit the growth of S. rolfsii. The highest inhibition was done by Micrococcus sp.4 (51.46 %). The physical and chemical properties of cassava rhizosphere soil affected the type of bacteria found in this study. The result confirmed that the potential isolates obtained from cassava rhizosphere soil can be plant growth promoters. The present study suggested that PGPR isolates might have potential in future field applications as plant growth promoters or biocontrol agents.
Temperature Effects on Jembrana Superficial Unit (JSU) Recombinant Protein Expression in Escherichia coli BL21 Maelani, Imelda; Indriawati, Indriawati; Ismail, Yulia Sari; Mulyani, Riska; Syahfitri, Widya
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64590

Abstract

The efficiency of expression of recombinant proteins in host cells depends on achieving proper protein folding, solubility, and biological activity. Critical environmental factors, including temperature, pH, nutrient availability, and inducer concentration, significantly influence protein yield, stability, and functionality. Optimizing these conditions is essential to minimize protein misfolding and aggregation, which can adversely affect expression efficiency and reduce bioactivity. This study aims to measure the effect of temperature on the growth of Escherichia coli BL21 harboring the recombinant JSU protein, derived from the ENV-SU gene of the Jembrana virus. A controlled laboratory experiment was conducted in which bacterial cultures were incubated at 25°C and 37°C under identical conditions, except for temperature. The results indicate a statistically significant difference in bacterial growth between the two temperature conditions (p = 0.08), suggesting that E. coli BL21 exhibits enhanced growth at 37°C compared to 25°C. The mean bacterial growth rate at 37°C was 1.756, whereas at 25°C, the average growth was 1.425, demonstrating that higher temperatures facilitate bacterial proliferation. However, due to the relatively small sample size, further validation with a larger dataset is required to confirm these findings. These findings highlight the critical role of temperature optimization in enhancing bacterial growth and improving recombinant protein production efficiency, emphasizing the need for further investigation with a larger dataset to validate the results. Keywords: recombinant protein; temperature; IPTG; Escherichia coli BL21; JSU construct.
ECTOMYCORRHIZAL MUSHROOMS DIVERSITY IN SORAYA RESEARCH STATION LEUSER ECOSYSTEM Rahmadani, Ayu; Rasnovi, Saida; Ismail, Yulia Sari
Biotik Vol 11 No 2 (2023): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v11i2.19268

Abstract

Ectomycorrhizal macro fungi have a mutualistic symbiosis with several plants, including those from the Dipterocarpaceae, Myrtaceae, and Fagaceae families. The existence of ectomycorrhizal macro fungi in nature is very important to preserve, therefore this study was conducted to determine the diversity of ectomycorrhizal macro fungi at Soraya Research Station. This study aims to determine the diversity of ectomycorrhizal macro fungi at the Soraya Research Stasiun Subulussalam, Leuser Ecosystem Area. The study was conducted from August 2021 to January 2022. The research method used is an exploratory survey, sampling using the squared method, using a permanent phenological plot of 20 plots measuring 20 m x 20 m, which are randomly placed in the secondary forest of the Soraya Research Station. The results obtained consisted of 5 orders, 14 families, 31 species, and 1440 individual numbers of ectomycorrhizal macro fungi. The most numerous species are obtained from the Boletaceae family, which is 5 species, and the Russulaceae family is 4 species. The ectomycorrhizal macro fungi species diversity index is relatively moderate at 2,997.
Co-Authors Cut Yulvizar Indriawati, Indriawati Lenni Fitri Maelani, Imelda Mulyani, Riska Putriani, Putriani Putriani, Putriani Rahmadani, Ayu Ryan Moulana Safriani, Suri Raihan Saida Rasnovi Syahfitri, Widya Warzatullisna, Warzatullisna