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All Journal Indonesian Journal of Halal Research Indonesian Community Journal
Maulidiyah, Hikmah
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Tofu Whey-Based Media for Probiotic Lactiplantibacillus plantarum D4 as a Halal Starter Culture Maulidiyah, Hikmah; Mustafa, Irfan; Jatmiko, Yoga Dwi; Ibrahim, Salam A.
Indonesian Journal of Halal Research Vol 6, No 1 (2024): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v6i1.30588

Abstract

In recent years, the development of probiotic-based fermented products with halal status has been a concern. The use of growth medium has relied on de Man Rogosa and Sharpe (MRS) as a relatively expensive standard medium, and its halal status is still uncertain. Extensive research has been carried out to investigate the development of low-cost halal alternative media for the cultivation of probiotic lactic acid bacteria (LAB). This study aimed to develop a probiotic halal and low-cost culture medium using a tofu whey-based medium. This study used three tofu whey-based media – A (tofu whey 100%), B (tofu whey 94.5%, molasses 3%, skim milk 2.5%), C (tofu whey 92.5%, molasses 3%, cheese whey 2.5%, tomato extract 2%), and MRS broth as a standard medium. Bacterial populations, total sugars utilized, total lactic acids produced, low pH (2.0) tolerance, and high bile salt concentration (oxgall 1.5%) were assayed. The highest bacterial population after 48 h of incubation was shown by medium B compared to medium MRS (12.34 ± 0.87 and 11.48 ± 0.3 log CFU/mL). Total sugars utilized by 48.28 ± 2.89, 38.89 ± 6.94, 39.14 ± 4.24, and 76.00 ± 1.41 %; on the other hand, total lactic acids produced by 0.16 ± 0.12, 0.03 ± 0.04, 0.31 ± 0.03, and 2.25 ± 1.48 % in A, B, C, and MRS, respectively. Probiotic tolerance at low pH and the presence of bile salts of Lactiplantibacillus plantarum D4 consistently showed a high survival rate in medium B compared to MRS. Based on these results, the components and proportions used in medium B were suitable for the growth of L. plantarum D4 as a halal probiotic starter candidate.
Tofu Whey-Based Media for Probiotic Lactiplantibacillus plantarum D4 as a Halal Starter Culture Maulidiyah, Hikmah; Mustafa, Irfan; Jatmiko, Yoga Dwi; Ibrahim, Salam A.
Indonesian Journal of Halal Research Vol. 6 No. 1 (2024): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v6i1.30588

Abstract

In recent years, the development of probiotic-based fermented products with halal status has been a concern. The use of growth medium has relied on de Man Rogosa and Sharpe (MRS) as a relatively expensive standard medium, and its halal status is still uncertain. Extensive research has been carried out to investigate the development of low-cost halal alternative media for the cultivation of probiotic lactic acid bacteria (LAB). This study aimed to develop a probiotic halal and low-cost culture medium using a tofu whey-based medium. This study used three tofu whey-based media - A (tofu whey 100%), B (tofu whey 94.5%, molasses 3%, skim milk 2.5%), C (tofu whey 92.5%, molasses 3%, cheese whey 2.5%, tomato extract 2%), and MRS broth as a standard medium. Bacterial populations, total sugars utilized, total lactic acids produced, low pH (2.0) tolerance, and high bile salt concentration (oxgall 1.5%) were assayed. The highest bacterial population after 48 h of incubation was shown by medium B compared to medium MRS (12.34 ± 0.87 and 11.48 ± 0.3 log CFU/mL). Total sugars utilized by 48.28 ± 2.89, 38.89 ± 6.94, 39.14 ± 4.24, and 76.00 ± 1.41 %; on the other hand, total lactic acids produced by 0.16 ± 0.12, 0.03 ± 0.04, 0.31 ± 0.03, and 2.25 ± 1.48 % in A, B, C, and MRS, respectively. Probiotic tolerance at low pH and the presence of bile salts of Lactiplantibacillus plantarum D4 consistently showed a high survival rate in medium B compared to MRS. Based on these results, the components and proportions used in medium B were suitable for the growth of L. plantarum D4 as a halal probiotic starter candidate.
Sosialisasi Pengenalan Siklus Aedes aegypti untuk Meningkatkan Kesadaran Pencegahan Demam Berdarah Dengue pada Siswa SDN 1 Sungai Besar Banjarbaru Muhamat, Muhamat; Gafur, Abdul; Rakmawati, Rakmawati; Anitasari, Lucia Desti; Taufiq, Hameda Dhaka Kusuma; Maulidiyah, Hikmah
I-Com: Indonesian Community Journal Vol 5 No 3 (2025): I-Com: Indonesian Community Journal (September 2025)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/i-com.v5i3.7698

Abstract

The Aedes aegypti mosquito is a vector for transmitting the dengue virus, which causes dengue hemorrhagic fever (DHF). This community service program was carried out by providing outreach and mentoring regarding the bioecology of Ae. aegypti to students of SDN 1 Sungai Besar, Banjarbaru. The limited knowledge of the school community, especially the students of SDN 1 Sungai Besar, about vector mosquitoes and their bioecology makes them less concerned about the environment. Systematic knowledge about DHF must be implemented from an early age to help raise awareness of the importance of sanitation in preventing the home and school environment from becoming mosquito habitats. Pre-test and post-test questionnaires were used to measure students' understanding of the bioecology of Ae. aegypti mosquitoes and were given to 25 students. Based on data analysis, it can be concluded that the outreach and mentoring program is effective and can help increase students' knowledge of the importance of sanitation in preventing the home and school environment from becoming mosquito habitats. This activity needs to be carried out in schools in Banjarbaru City so that the school environment does not become a habitat for Ae. aegypti mosquitoes.
Co-Authors Abd. Gafur Anitasari, Lucia Desti Ibrahim, Salam A. Irfan Mustafa Muhamat Muhamat Rakmawati, Rakmawati Taufiq, Hameda Dhaka Kusuma YOGA DWI JATMIKO