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All Journal AGRIEKONOMIKA Indonesian Journal of Halal Research
Maulidiyah, Hikmah
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Medicine & Pharmacology Other

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Tofu Whey-Based Media for Probiotic Lactiplantibacillus plantarum D4 as a Halal Starter Culture Maulidiyah, Hikmah; Mustafa, Irfan; Jatmiko, Yoga Dwi; Ibrahim, Salam A.
Indonesian Journal of Halal Research Vol. 6 No. 1 (2024): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v6i1.30588

Abstract

In recent years, the development of probiotic-based fermented products with halal status has been a concern. The use of growth medium has relied on de Man Rogosa and Sharpe (MRS) as a relatively expensive standard medium, and its halal status is still uncertain. Extensive research has been carried out to investigate the development of low-cost halal alternative media for the cultivation of probiotic lactic acid bacteria (LAB). This study aimed to develop a probiotic halal and low-cost culture medium using a tofu whey-based medium. This study used three tofu whey-based media - A (tofu whey 100%), B (tofu whey 94.5%, molasses 3%, skim milk 2.5%), C (tofu whey 92.5%, molasses 3%, cheese whey 2.5%, tomato extract 2%), and MRS broth as a standard medium. Bacterial populations, total sugars utilized, total lactic acids produced, low pH (2.0) tolerance, and high bile salt concentration (oxgall 1.5%) were assayed. The highest bacterial population after 48 h of incubation was shown by medium B compared to medium MRS (12.34 ± 0.87 and 11.48 ± 0.3 log CFU/mL). Total sugars utilized by 48.28 ± 2.89, 38.89 ± 6.94, 39.14 ± 4.24, and 76.00 ± 1.41 %; on the other hand, total lactic acids produced by 0.16 ± 0.12, 0.03 ± 0.04, 0.31 ± 0.03, and 2.25 ± 1.48 % in A, B, C, and MRS, respectively. Probiotic tolerance at low pH and the presence of bile salts of Lactiplantibacillus plantarum D4 consistently showed a high survival rate in medium B compared to MRS. Based on these results, the components and proportions used in medium B were suitable for the growth of L. plantarum D4 as a halal probiotic starter candidate.
Restoring the Meratus: a permaculture-based nexus supporting adaptive practice in indigenous communities Muhamat, Muhamat; Utari, Nofi; Anitasari, Lucia Desti; Maulidiyah, Hikmah; Krisdianto, Krisdianto
Agriekonomika Vol 15, No 1: APRIL 2026 (In Press)
Publisher : Department of Agribusiness, Faculty of Agriculture, Universitas Trunojoyo Madura, Indonesi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agriekonomika.v15i1.33810

Abstract

This study investigates the role of permaculture-based agroforestry in strengthening ecosystem restoration, food security, and community resilience within the indigenous Dayak Meratus population in South Kalimantan, Indonesia. Following a major flash flood in 2021, an integrated landscape management initiative was implemented in Hinas Kiri village. From January to June 2025, data from 100 respondents collected through modified snowball sampling revealed measurable improvements in ecological and socio-economic indicators. The implementation of agroforestry systems based on permaculture principles resulted in increased plant diversity, improved soil conditions, and positive trends in tree growth Biophysical assessments showed increased tree canopy cover and trunk diameter (5.7–7.1%) across Shorea sp., Peronema canescens, and Hevea brasiliensis, while crop yields rose for banana (+8.0%), taro (+8.9%), and rattan (+12.5%). Grounded theory analysis indicated a perceptual shift among farmers, who increasingly viewed permaculture as a climate adaptation strategy and pathway to economic diversification. A digital monitoring prototype and preliminary policy brief were developed to support adaptive management and evidence-based decision-making. The findings provide empirically grounded insights and demonstrate the value of an integrative nexus-based approach for climate-resilient landscape governance in rural area.
Co-Authors Anitasari, Lucia Desti Ibrahim, Salam A. Irfan Mustafa Krisdianto Sugiyanto Muhamat Muhamat Utari, Nofi YOGA DWI JATMIKO