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All Journal Budapest International Research in Exact Sciences (BirEx Journal)
Sri Nevi Gantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Physics Transportation

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Response Surface Methodology in determining the Temperature and Drying Time of Lactobacilus Casei Probiotic Granules Sri Nevi Gantini; Ari Widayanti
Budapest International Research in Exact Sciences (BirEx) Journal Vol 4, No 3 (2022): Budapest International Research in Exact Sciences, July
Publisher : Budapest International Research and Critics University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/birex.v4i3.5738

Abstract

Lactobacillus casei is a probiotic bacteria that has an important role in maintaining body health, but its use in liquid preparations is still constrained by shelf life. This study aims to determine the effect of temperature and drying time of wet granulation method on the physical and viability of probiotic granules. This research begins with increasing bacterial biomass and then harvesting the bacteria using centrifuge. Bacteria are encapsulated using 2% alginate and mannitol as fillers. It was dried at a temperature and time variation of 13 test formulas that had been designed by central composite design. The optimal temperature and drying time of probiotic granules obtained from Response Surface Methodology is 45 ° C for 2 hours.
Co-Authors Ari Widayanti