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Improvement of experimental animal handling skills for higher education laboratory technicians in Semarang Region Indonesia Marianti, Aditya; Christijanti, Wulan; Isnaeni, Wiwi; Sholihah, Badingatus; Widyaningrum, Kartika; Anggoro, Naufal Sebastian; Priyanto, Yudi
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol 5, No 1 (2021)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v5i1.3670

Abstract

Laboratory technician skills in handling experimental animals are needed by the study program, which uses experimental animals in the lecture and research process. The Biology Laboratory of FMIPA UNNES has the resources to practice these skills. As the realization of the 3rd Dharma of the Tri Dharma of Higher Education, community service is carried out to improve the skills of higher education laboratory technicians both private and public universities in the city of Semarang in handling experimental animals. The method used is training in the form of hands-on practice. The animals used were mice and white rats. The trained practices were bioethics, maintenance techniques, handling, gavage, drawing blood, and vaginal smears of experimental animals. The participants consisted of 29 laboratory technicians from general medicine, dentistry, biology, biology education, pharmacy, and animal husbandry study programs. The training instructors consist of lecturers, technicians, and student assistants. The training was held for two days by implementing health protocols. As a result, the participants improved their skills. From the beginning, only 29,26% of participants had the knowledge and skills to handle experimental animals at the end of the training, increasing to 86.84%. It concludes that the participants have increased their knowledge and skills in handling experimental animals.
Antihyperlipidemic Activity of Tamarind (Tamarindus indica) Extract on the Lipid Profile of Albino Rats Christijanti, Wulan; Susanti, R.; Rakainsa, Senda Kartika; Widyaningrum, Kartika; Mohamed, Mohd Salim Bin
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.7579

Abstract

Tamarind are widely used as antioxidants, anti-diabetes, anti-hyperlipidemia, and anti-cholesterolemia. The aim of this research is to analyze the anti-hyperlipidemic activity of extracts of tamarind fruit pulp, seeds and leaves on the lipid profile of rats. Experimental research was designed with 20 male rats divided   Control (C), Tamarind leaves (TL), Tamarind fruit pulp (TF), and Tamarind seeds (TS) extracts, each 500 mg/kg of body weight. All of the groups were given standard feed and induced with lard at 3 ml/rat/day for 14 days. On day 15, the rats were continued to be induced with lard and were given extracts according to each group for 2 weeks. The data obtained such as rats’ body weight, level of cholesterol, HDL (high-density lipoprotein), LDL (low-density lipoprotein), triglycerides (TG) and Malondialdehyde (MDA)  were analyzed using anova (P<0.05) and the Duncan Multiple Range Test (DMRT). The results showed that each extract had a significant effect on cholesterol, HDL and MDA levels but not on body weight, LDL and triglycerides. Levels of MDA in the group with the extract were significantly lower than the control. Tamarind pulp, seeds and leaf extracts show antihyperlipidemic activity by reducing cholesterol and HDL levels. This can be the basis for using parts of the tamarind plant as a supplement for the community to maintain fat levels.