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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat International Journal of Cell and Biomedical Science
Anggoro, Naufal Sebastian
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Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Improvement of experimental animal handling skills for higher education laboratory technicians in Semarang Region Indonesia Marianti, Aditya; Christijanti, Wulan; Isnaeni, Wiwi; Sholihah, Badingatus; Widyaningrum, Kartika; Anggoro, Naufal Sebastian; Priyanto, Yudi
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol 5, No 1 (2021)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v5i1.3670

Abstract

Laboratory technician skills in handling experimental animals are needed by the study program, which uses experimental animals in the lecture and research process. The Biology Laboratory of FMIPA UNNES has the resources to practice these skills. As the realization of the 3rd Dharma of the Tri Dharma of Higher Education, community service is carried out to improve the skills of higher education laboratory technicians both private and public universities in the city of Semarang in handling experimental animals. The method used is training in the form of hands-on practice. The animals used were mice and white rats. The trained practices were bioethics, maintenance techniques, handling, gavage, drawing blood, and vaginal smears of experimental animals. The participants consisted of 29 laboratory technicians from general medicine, dentistry, biology, biology education, pharmacy, and animal husbandry study programs. The training instructors consist of lecturers, technicians, and student assistants. The training was held for two days by implementing health protocols. As a result, the participants improved their skills. From the beginning, only 29,26% of participants had the knowledge and skills to handle experimental animals at the end of the training, increasing to 86.84%. It concludes that the participants have increased their knowledge and skills in handling experimental animals.
Profiling of Seawater Bacterial Diversity in Tanjung Mas Port Using 16S rRNA eDNA Metabarcoding and Next-Generation Sequencing (NGS) Anggoro, Naufal Sebastian; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto; Wijanarka, Wijanarka; Zainuri, Muhammad; Hadibarata, Tony
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 1 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.1.61-70

Abstract

Tanjung Emas Port is the entry and exit point for trade commodities, both regional and international filled with many ships. This condition makes biofouling a very massive process in that place by various types of marine bacteria. The initial formation of a biofilm is relevant to bacterial diversity, colonization and adhesion. The objective of the study was assessing bacterial diversity in relation to with biofouling within Tanjung Mas Port Semarang, by using 16S rRNA eDNA metabarcoding Next-Generation Sequencing (NGS). Seawater samples from aquatic sites of Tanjung Mas harbor was used for DNA extraction and amplification of the 16S rRNA V-3-V4 hypervariable region, followed by sequencing and library construction of eDNA Metabarcoding. Sequence processing and analysis was performed in QIIME 2 and RStudio using DADA2 for advanced sequencing processing and Phyloseq. The results of this research showed that bacteria is the predominant taxon constituting 100% of the community. The taxon consists of Proteobacteria (49.38%), Bacteroidota (8.67%), and Firmicutes (8.88%). Alphaproteobacteria (20.92%) and Gammaproteobacteria (12.39%) dominate at the Class level, emphasizing their versatility and ecological influence. At the Order and Family levels reveals the prevalence of Rhodobacterales (10.04%), Chitinophagales (2.53%), Rhizobiales (3.61%), Rhodobacteraceae (5.67%), Saprospiraceae (3.63%), and Rhizobiaceae (2.15%). It was found that the unculturable taxa dominance in Tanjung Mas Port was 44.66%. These taxonomic entities contribute significantly to the taxonomic and functional diversity of the microbial community, influencing nutrient cycling, organic matter degradation, ecosystem stability and biofilm formation.
hUC-MSCs Therapy with EVs Booster Improves Recovery in Stage 2 Chronic Kidney Disease with Hypercholesterolemia : a case report Jutadi; Ayu, Dian Respati; Cahyani, Dini; Arda, Adzani Gaisani; Anggoro, Naufal Sebastian
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.63

Abstract

Hypercholesterolemia is a common metabolic comorbidity that accelerates the progression of chronic kidney disease (CKD). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and their secretome, which consist of extracellular vecicles (EVs) and soluble bioactive molecules, have shown potential in modulating inflammation and metabolism. This case report describes significant improvement in serum lipid profile following hUC-MSC and secretome therapy in a patient with stage 2 CKD and hypercholesterolemia. A male patient with CKD stage 2 received two intravenous cycles of hUC-MSC and secretome therapy administered seven months apart. Serial evaluations demonstrated a progressive decline in total cholesterol from 294 mg/dL at baseline to 286 mg/dL after the first treatment and 225 mg/dL after the second. LDL cholesterol decreased from 188 mg/dL to 140 mg/dL, with a mild rebound to 175 mg/dL. HDL cholesterol, initially elevated at 214 mg/dL, showed a modest increase to 220 mg/dL after the first treatment, followed by normalization to 175 mg/dL. Triglyceride levels remained within the normal range (44–51 mg/dL) throughout the observation period. The marked improvement in lipid parameters suggests that hUC-MSC and secretome therapy may exert systemic metabolic regulation via anti-inflammatory, antioxidative, and hepatoprotective mechanisms. hUC-MSC and secretome administration demonstrated potential benefits in lipid homeostasis in a patient with CKD and hypercholesterolemia. These findings support the role of MSC-derived secretome as a promising adjunctive therapeutic approach. Larger controlled trials are warranted to confirm these outcomes and elucidate underlying mechanisms.
Time-Dependent Simulation Identifies Critical Hour Phase of Intestinal Acute Injury in Sepsis Mouse Model Istanti, Yusrina; Anggoro, Naufal Sebastian; Husain, Sofian Azalia; Azzahara, Salma Yasmine
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.64

Abstract

Background: Understanding the dynamic process of intestinal injury and repair during sepsis is essential for identifying optimal therapeutic windows. This study aimed to determine the critical time phase of intestinal acute injury by analyzing histological changes over a 24-hour period in a sepsis mouse model. Methods: Mice were divided into four groups—Control, 9 h, 12 h, and 24 h—and intestinal tissue samples were assessed using the Chiu histological scoring system. A time-dependent simulation was conducted to evaluate average changes in tissue damage and to identify key transition points between injury and recovery phases. Statistical analysis was performed using one-way ANOVA followed by post hoc comparisons to determine significant differences among time points. Results: The simulation demonstrated a marked increase in intestinal damage between 9 h and 12 h, followed by partial recovery at 24 h. Statistical analysis revealed a significant difference (p < 0.05) corresponding to this shift. These findings suggest that peak tissue injury occurs around 12 hours post-sepsis induction, preceding the onset of repair mechanisms. Conclusion: The study provides quantitative insight into the temporal progression of intestinal injury in sepsis, identifying the 12–24 hour period as a critical therapeutic window for potential interventions.
Human-Umbilical Cord-Mesenchymal Stem Cells (hUC-MCSs) Therapy with Extravesicles (EVs) Booster Improves Recovery in Type 2 Diabetes Mellitus with Cardiovascular Disease Nugraha, Dendi Krisna; Jutadi; Anggoro, Naufal Sebastian; Sari, Fikriya Novita; Ardani, Yanuar
International Journal of Cell and Biomedical Science Vol 4 No 10 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i10.66

Abstract

Background: Type 2 diabetes (T2DM) is a chronic metabolic disorder characterized by insulin resistance and β-cell dysfunction, leading to persistent hyperglycemia and complications. Studies have explored mesenchymal stem cell (MSC)-based therapies and their extracellular vesicles (EVs) as novel approaches for metabolic regulation and tissue repair. Case: A 43-year-old male patient exhibited symptoms including excessive thirst and hunger, frequent urination, fatigue, and intermittent blurry vision. He had type 2 diabetes and recently worsened symptoms. The obese patient had elevated blood glucose, HbA1c, triglycerides, and uric acid. He received umbilical cord-derived mesenchymal stem cells (161,6 × 106 cells), followed by seven intramuscular EV injections (1.5 cc each), along with diet and antioxidant supplements. Results: Three months after the conclusion of treatment, laboratory test showed significant improvement, with fasting glucose levels measuring at 91 mg/dL, HbA1c levels at 5,1%, triglyceride levels at 151 mg/dL, uric acid levels at 4,9 mg/dL, and an erythrocyte sedimentation rate of 12 mm/hr. The clinical symptoms such as nocturia, fatigue, and neuropathic pain, demonstrated a substantial improvement, as well as led to the resolution of skin xerosis and heel fissures. Conclusion: This case suggests that combined UC-MSC and EV therapy, complemented by lifestyle modification, may contribute to metabolic stabilization and symptomatic relief in T2DM patients.
Co-Authors Aditya Marianti Anto Budiharjo Arda, Adzani Gaisani Ardani, Yanuar Ayu, Dian Respati Azzahara, Salma Yasmine Cahyani, Dini Hermin Pancasakti Kusumaningrum Husain, Sofian Azalia Jutadi Muhammad Zainuri Nugraha, Dendi Krisna Priyanto, Yudi Sari, Fikriya Novita Sholihah, Badingatus Tony Hadibarata, Tony Widyaningrum, Kartika Wijanarka Wijanarka Wiwi Isnaeni Wulan Christijanti Yusrina Istanti