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Arjuna, Mesha
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Energy Engineering Environmental Science Materials Science & Nanotechnology Mathematics Mechanical Engineering

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Isolation of Cellulolytic Microbes from Bio-Slurry Muammar, Arief; Manullang, Maria; Arjuna, Mesha; Retnaningrum, Endah
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 1 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.564 KB) | DOI: 10.24036/eksakta/vol22-iss1/257

Abstract

Cellulolytic microbes produce cellulase enzymes that can degrade cellulose. Cellulolytic microbes are found in many habitats, including in cattle waste (bio-slurry), which is result of hydrolysis of cattle feed ingredients sourced from cellulose. Cellulase enzymes play a role in the process of hydrolyzing cellulose into glucose. This study aims to isolate cellulolytic microbes from bio-slurry and measure activity of cellulase enzymes in cellulolytic microbes isolated from bio-slurry. We are taking bio-slurry samples with 5 sample points and isolating the microbes in Carboxyl Methyl Cellulose (CMC) medium, cellulase enzyme activity qualitative test with Congo Red Assay, and perform quantitative test with the DNS Assay. Microbial isolates which shows positive results for cellulolytic activity in the qualitative test called SIIC1, SIIIC5, SIIC3. In the quantitative test can be seen based on Optical Density (OD) for cell lysate, SIIC1 0.298; SIIC3 0.186; SIIIC5 0.247; and a 0.332 for blank solution. Whereas supernatant obtain SIIC1 value of 0.237; SIIC3 0.212; SIIIC5 0.198; and 0.195 for blank solution. Based on the results, it can be concluded that the cellulolytic activity of microbes in bio-slurry based on OD values shows good results in supernatant, but it’s not significant enough in the cell lysate.
Cellulolytic Bacteria Isolation from Sugarcane Garden Soil Muammar, Arief; Arjuna, Mesha; Manullang, Maria; Retnaningrum, Endah
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 27 No. 01 (2026): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464) In Progress
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol27-iss01/632

Abstract

The soil surrounding the sugar cane garden is a promising habitat for cellulolytic bacteria because it contains a source of cellulose, hemicellulose, and other carbon sources derived from the sugar canes remaining organic matter.  This study attempts to determine the presence of cellulolytic bacteria isolated from sugar cane garden soil, as well as the ability of cellulolytic bacteria to degrade cellulose based on optical density and sugar reduction values isolated from sugarcane garden soil.  We collected soil samples from five points (T1, T2, T3, T4, T5), isolating the bacteria in CMC (Carboxyl Methyl Cellulose) medium, qualitatively testing the cellulase enzyme activity with Congo Red and Iodine Assays, and quantitatively using the DNS Assay. The results of the qualitative test with the Congo Red assay revealed that there were three positive isolates with a clearly visible clear zone, namely TII C4, TII C1, and TIV C4, and after quantitative supernatant tests, the OD values of all isolates were higher than the negative control in supernatant samples using 540nm wave length using spectrophotometer, by using synthetic cellulose called Carboxy Methyl cellulase (CMC) as a substrate in the cellulase enzyme test.
Co-Authors Manullang, Maria Muammar, Arief Retnaningrum, Endah