Article Per Year (5 Year)
p-Index From 2020 - 2025
2.195
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Sains Dasar Jurnal Teknosains Journal of Degraded and Mining Lands Management Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengolahan Pangan Eksakta : Berkala Ilmiah Bidang MIPA INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Narra J
Retnaningrum, Endah
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
KEMAMPUAN FERMENTASI BAL DENGAN SUBSTRAT SUSU KACANG MERAH Hastuti, Luthfiana Indah; Retnaningrum, Endah
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8856

Abstract

The oligosaccharide content in red beans can be broken down with groups of microorganisms, namely lactic acid bacteria. This decomposition is done by the fermentation process. Lactic acid bacteria used in this study were Lactococcus lactis subsp. lactis NBRC 12007. This study aims to determine BAL Lactococcus lactis subsp. Lactis NBRC 12007 in the fermentation process of red bean juice and regulates the difference in quality by fermentation of UHT cow's milk with the same starter bacteria. Beginner Lactococcus lactis subsp. lactis NBRC 12007 was added 3% to the substrate of red bean extract and UHT cow's milk. Fermentation is carried out for 24 hours at 37oC. The results showed that the total cell, total acid, total protein, pH, antibacterial and physical values were much better and improved the quality of the fermented red bean extract substrate product compared to the fermentation with UHT cow's milk substrate.
Isolasi dan Karakterisasi Bakteri Asam Laktat Penghasil Eksopolisakarida dari Buah Kersen (Muntingia calabura L.) Chika Giyatno, Divya; Retnaningrum, Endah
Jurnal Sains Dasar Vol 9, No 2 (2020): October 2020
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v9i2.34523

Abstract

Bakteri asam laktat (BAL) dapat memberikan manfaat dan aman untuk dikonsumsi manusia. Bakteri ini menghasilkan metabolit sekunder yang berpotensi menghasilkan eksopolisakarida (EPS). Bakteri asam laktat ditemukan pada buah-buahan salah satunya buah kersen (Muntingia calabura L.). Kandungan karbohidrat yang tinggi dalam buah ini cocok untuk pertumbuhan BAL. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri asam laktat beserta karakter fenotipik dan mengetahui potensinya dalam menghasilkan eksopolisakarida. Isolat bakteri asam laktat diperoleh dengan cara inokulasi suspensi buah kersen ke dalam medium MRS (de Man Rogosa Sharpe). Isolat bakteri asam laktat yang diperoleh kemudian dikarakterisasi fenotipik berdasarkan morfologi koloni, sel, sifat biokimiawi, dan fisiologis. Sebanyak empat isolat BAL diperoleh dengan bentuk sel basil, gram positif, katalase negatif, non-motil, non spore forming, mesofilik, aciduric, non halofilik serta dapat memfermentasi karbohidrat. Berdasarkan Bergey’s Manual of Systematic Bacteriology keempat isolat teridentifikasi sebagai spesies Lactobacillus plantarum. Keempat isoalat juga menunjukkan kemampuan menghasilkan eksopolisakarida dengan kisaran 870-1.910 mg/L.  
Examining the prevalence and antimicrobial resistance profiles of multidrug-resistant bacterial isolates in wound infections from Indonesian patients Prastiyanto, Muhammad E.; Darmawati, Sri; Daryono, Budi S.; Retnaningrum, Endah
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.980

Abstract

The emergence of multidrug-resistant (MDR) infections in wounds is a significant public health issue. The aim of this study was to investigate the prevalence and antimicrobial resistance profiles of MDR bacterial isolates in wound infections. Through a cross-sectional study, 1035 bacterial isolates were collected from wound infection patients at Tugurejo Hospital in Semarang, Indonesia, over a three-year period (January 2020 to December 2022). Initial identification involved Gram staining and colony morphology assessment, followed by biochemical assays and antimicrobial susceptibility testing using the VITEK®2 Compact system. Gram-negative bacteria constituted the majority of isolates (60.77%, n=629). The predominant strains included were Staphylococcus spp. (30.92%, n=320), Escherichia coli (18.45%, n=191), and Klebsiella pneumoniae (13.04%, n=135). Notably, Gram-negative bacteria exhibited a significantly higher likelihood of MDR development compared to their Gram-positive counterparts (p<0.001), with Gram-negative bacteria having a 2.05 times higher probability of acquiring MDR. These findings underscore the urgent need for comprehensive surveillance of antimicrobial resistance patterns and the implementation of tailored antimicrobial stewardship programs to address the pressing public health challenge of MDR wound infections. Further research is warranted to elucidate the complex interplay of factors contributing to MDR development in wound infections, thereby informing targeted intervention strategies and improving patient outcomes.
Degradation of diesel fuel by Pseudomonas aeruginosa B031 with expression of the alkB gene in a column bioreactor Daris, Pieter Jhon Joshua; Wilopo, Wahyu; Warmada, Wayan; Retnaningrum, Endah
Journal of Degraded and Mining Lands Management Vol. 12 No. 1 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.121.6875

Abstract

Diesel fuel pollutants contain toxic hydrocarbons dominated by aliphatic and aromatic hydrocarbons. Hydrocarbon pollutants can be degraded in an environmentally friendly manner through bioremediation using hydrocarbonoclastic bacteria, i.e., Pseudomonas aeruginosa B031, which harbors the alkB gene that encodes an alkane hydroxylase that degrades alkane chains in hydrocarbons. This study compared the ability and efficiency of P. aeruginosa B031 to degrade diesel pollutants in a batch system and a continuous system using a column bioreactor, as well as the expression of alkB. P. aeruginosa B031 could more efficiently degrade diesel fuel in a continuous system in a column bioreactor than in the batch system. The concentrations of phenol, total organic carbon, chemical oxygen demand, and biological oxygen demand in the column bioreactor underwent a greater decrease than those in the batch system, namely 1.5-fold, 1.7-fold, 1.4-fold, and 1.3-fold, respectively. The decrease in these concentrations was followed by changes in functional groups, as shown via Fourier transform infrared spectroscopy. The number of bacteria and the concentration of exopolysaccharide increased in the column bioreactor by 4-fold and 2.3-fold more than the increase in the batch system. The ability of P. aeruginosa B031 to degrade diesel fuel in the column bioreactor was also demonstrated by the higher expression of alkB than that in the control.
EFEKTIVITAS DAN KUALITAS PRODUK FERMENTASI KOMBUCHA DENGAN KOMBINASI SUBSTRAT TEH DAUN SUKUN (Artocarpus altilis (Parkinson) Fosberg) DAN LEMON (Citrus limon (L.) Burm. f.) Ardhelia Christy Anggraini; Retnaningrum, Endah
Jurnal Pengolahan Pangan Vol 8 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Alkhairaat Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/pangan.v8i2.118

Abstract

Daun sukun dapat dimanfaatkan sebagai minuman fungsional, salah satunya teh herbal yang kemudian dapat dibuat menjadi kombucha dan dikombinasikan dengan lemon. Penelitian ini bertujuan untuk mempelajari senyawa fenolik, organoleptik, nilai pH, persentase asam asetat, total gula, total mikrobia dan berat bioselulosa, serta efektivitas antibakteri kombucha teh daun sukun dan lemon. Cara kerja pada penelitian ini terdiri dari pembuatan kombucha, skrining fitokimia, pengujian organoleptik, perhitungan nilai pH, persentase asam total, gula total, dan pengujian aktivitas antibakteri. Hasil skrining fitokimia menunjukkan kombucha mengandung senyawa fenolik berupa flavonoid dan tanin. Hasil organoleptik menunjukkan produk kombucha yang difermentasi selama 15 hari dapat diterima dalam kategori suka dan agak suka. Nilai pH menurun sebesar 2,31-2,96, Persentase asam asetat meningkat sebesar 0,348-0,038%, gula total secara umum mengalami penurunan sebesar 1,42-10,90%, total bakteri meningkat dari hari ke-0 hingga 7 dan menurun pada hari ke-15, total khamir meningkat selama 15 hari, Bioselulosa hari ke-15 memiliki berat basah (127,73g) dan kering (35,21g). Kombucha memiliki efektivitas antibakteri terhadap bakteri Gram positif dan Gram negatif.
Black-Pigmented Marine Pseudomonas aeruginosa Exhibiting Anti-Bacterial Activity against Multidrug-Resistant (MDR) Wound Infection Bacteria Prastiyanto, Muhammad Evy; Darmawati, Sri; Daryono, Budi Setiadi; Retnaningrum, Endah
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.880-890

Abstract

The urgency of multidrug-resistant (MDR) bacterial infections in wounds is a significant concern due to the high prevalence of MDR in healthcare settings. Black pigmented marine bacteria, strain PS1C, were isolated from marine sediment on Awur Beach Jepara, Central Java, Indonesia, and cultured in the laboratory. The aim of this research includes molecular identification of strain PS1C, extracting black pigment from strain PS1C, isolation of MDR bacteria from wounds, and evaluating the antibacterial activity of black pigment from strain PS1C against MDR bacteria isolates of wound infections. We examined the 16S rRNA gene sequences strain PS1C to identify the species. Then, the black pigment from PS1C was extracted using methanol: acetone (7:3) solvent. Antibacterial activity was evaluated against MDR bacteria from wounds with the microdilution method. A black-pigmented bacterium was isolated and identified as Pseudomonas aeruginosa PS1C. We found that the black pigment from P. aeruginosa PS1C can be developed as an antibacterial agent against the MDR bacterial isolate of wounds with MIC and MBC values of 6.25-12.5 mg/ml and 6.25-25 mg/ml, respectively. In conclusion, the study's findings highlight the potential of the extract of black pigment from P. aeruginosa PS1C as an antibacterial agent against wound-causing MDR bacteria and reinforce previous research into P. aeruginosa can be isolated from marine sources. Additional in vivo investigations and the identification of the antibacterial activity's mode of action are required.
Identification of Marbofloxacin-Susceptible Bacterial Uropathogens in Feline Urinary Tract Infection of Different Age Groups Udasmara, Sekarani Arifah; Retnaningrum, Endah
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.575-584

Abstract

Urinary tract infections (UTI), an adhesion and multiplication of bacterial uropathogens in the urogenital system, are one of the most important indications for antimicrobial use in veterinary medicine and contribute to the development of antimicrobial resistance. Previously, it was revealed that older cats with an average of 9.1 years old are most likely to develop UTI, however a contradicting investigation stated kittens and young adults are more likely to contract UTI. Along with the uprising of amoxicillin- and ampicillin-resistant uropathogens, the study aims to turn towards marbofloxacin reserve and to analyze their potency. Furthermore, the current study aims to enumerate and compare the number of uropathogens between age groups. Enumeration and identification of uropathgoens from urine samples of feline UTI patients were was conducted through the urine culture on EMB, MSA, and BHI medium followed by an IMViC test and inoculation to the Mueller-Hinton agar to observe the zone of inhibition. The investigation revealed that a higher number of younger cats and kittens were reported to have UTI and catheter-induced UTI with a higher number of uropathogens in the population. The uropathogens were further identified as Staphylococcus spp., Enterococcus spp., Enterobacter spp., Escherichia spp., and Klebsiella spp. Additionally, marbofloxacin was found to be effective in eliminating and inhibiting the growth of these uropathogens. In conclusion, younger cats were observed to be more prone to UTI of a diverse range of uropathogens, however, marbofloxacin may aid in the treatment of the cats as it has been proven to be most effective.
Detection of Antibacterial Susceptible Salmonella spp. in Cured Beef with Different Shelf-Life Expectancy Khairunisa, Mutia Asri; Retnaningrum, Endah
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.467-473

Abstract

Meat is currently one of the food products with the highest demand ever since 1980, where demand has reached 24.8 kg per year per person as of 2020. With high demand comes intensive farming which causes overuse of antimicrobials for both therapeutic and non-therapeutic reasons, allowing more antimicrobial resistant (AMR) strains of bacteria to occur. In the case of meat products, salmonella is considered one of the more commonly occurring bacteria found in raw meat products. However, with meat’s short shelf-life expectancy, the likeliness of consumers suffering from salmonellosis increases. Thus, preservation methods have been implemented to reduce this likeliness, primarily through curing beef. Although curing may reduce the likeliness of excessive microbial growth, AMR salmonella has been detected in cured beef samples. The scope of this research determines whether there is Salmonella spp. within the cured beef samples, conduct AMR (azithromycin, ciprofloxacin, and ceftriaxone) analysis of the Salmonella spp. isolated from the cured beef samples and enumeration was conducted. The longer the shelf-life expectancy of cured beef samples, the lower the overall CFU/mL per sample was (control: 17,800,000 CFU/mL, >1 year: 0 CFU/mL). It was also discovered that Salmonella spp. has potential resistance towards ciprofloxacin (33.33% intermediate) and susceptibility towards azithromycin and ceftriaxone (100.00% sensitive). This research implies the agricultural industry and safety for consumers of cured beef products with different shelf-life expectancies.
Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Soleha, Siti; Syarifah, Syarifah; Nurseha, Tito; Fatiqin, Awalul; Retnaningrum, Endah; Serang, Yitro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Fermented Feed for Laying Hens: Effects on Egg Quality and the Number of Enteric Pathogenic Bacteria Present Amantsura, Daimeera Anja Lulu; Retnaningrum, Endah
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.37-41

Abstract

Eggs are one of the most common food sources for human consumption because they are common, easy to obtain, and nutritious. Eggs contain abundant vitamins and minerals that the human body needs, are a good source of protein and fat and are rich in choline. Despite its high nutritional value, eggs are also one of the leading causes of food poisoning and foodborne diseases in various countries. This is due to contamination in eggs by pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Campylobacter bacteria. Fermented chicken feed contains natural probiotics added to the feed, which improves gut health, better absorption of nutrients, inhibits the growth of pathogenic bacteria, enhances the immune response in chickens. This study is conducted to distinguish the diffrence in quality of eggs that can be physically observed in chicken eggs fed with fermented feed compared to chicken eggs fed regular feed, as well as to compare the number of total bacteria and enteric pathogenic bacteria contained in the contents of chicken eggs that includes Escherichia coli and Salmonella spp bacteria. The methods used in this research include observing egg quality comparisons, calculating and comparing the total number of bacteria, and testing for the presence of enteric pathogenic bacteria Escherichia coli and Salmonella, spp. using the plate count method. The resulting data was processed using the one-way ANOVA method with a confidence level of 95%. The results of this research show higher egg quality in fermented fed eggs which is characterized by the color of the yolk which tends to be darker yellow to orange and thicker egg shells. The total bacteria count showed that the bacteria was significantly lower in eggs from chickens fed fermented feed. Tests for the number of enteric bacteria showed positive results for E. coli in 1 out of 10 egg content samples from chicken fed with fermented feed, and positive results for E. coli in 2 out of 10 egg samples from chicken fed with regular feed, while test results for Salmonella spp. showed negative results in all samples.
Co-Authors Amantsura, Daimeera Anja Lulu Ardhelia Christy Anggraini Arjuna, Mesha Atmandaru, Susmaya BUDI SETIADI DARYONO Burhanuddin Ihsan, Burhanuddin Chika Giyatno, Divya Daris, Pieter Jhon Joshua Daryono, Budi S. Fatiqin, Awalul Hastuti, Luthfiana Indah Khairunisa, Mutia Asri Manullang, Maria Muammar, Arief Nurseha, Tito Prastiyanto, Muhammad E. Prastiyanto, Muhammad Evy Serang, Yitro Soleha, Siti Solichah, Amalia Sri Darmawati Syarifah Syarifah, Syarifah Udasmara, Sekarani Arifah Wahyu Wilopo Warmada, Wayan Yulma, Yulma