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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. The Indonesian Biomedical Journal
Handirosiyanto, Ikhwan
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Effectiveness of Petai (Parkia speciosa) Ethanol Extract in Controlling Foam Cell Numbers in Atherosclerosis-Induced Male Wistar Rats Handirosiyanto, Ikhwan; Indra setiawan; Nisa, Seftiana Khaerun; Lestari, Dian Yuliartha
Saintika Medika Vol. 20 No. 1 (2024): June 2024
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/sm.Vol20.SMUMM1.38652

Abstract

Atherosclerosis is a chronic inflammatory disease that causes the narrowing of the arterial lumen, and it is considered the primary cause of cardiovascular diseases such as myocardial infarction (MI), heart failure, and stroke. Cardiovascular disease is the leading of death globally. Atherosclerosis has a complex pathophysiology, such as endothelial dysfunction, thickening of the tunica intima, and the formation of atheromatous plaques. After that process, foam cells will form, which is the key to the development of atherosclerosis. Petai ethanol extract contains antioxidants, which are flavonoids, saponins, and tannins, that can reduce the number of Low-Density lipoproteins (LDL). The purpose of this study was to evaluate the effect of petai ethanol extract on the number of atherosclerotic foam cells in male white rats (Rattus norvegicus strain wistar) induced by lard. The method of this study was a genuinely experimental and post-test-only control group design with 25 rats divided into five control groups, which are one negative control group, one positive control group, and three treatment groups with extract doses of 100mg/kgBW, 200mg/kgBW and 400mg/kgBW. Observation of foam cells was carried out using a microscope with 400x magnification. Data were analyzed using Oneway ANOVA and Post Hoc Tamhane's test. The results of the study using the Oneway ANOVA test showed that there were significant differences between groups (p: 0.000). Post Hoc Tamhane's test between the positive control group and treatment groups 1, 2, and 3 can be seen that there is a significant difference between the formation of foam cells due to the administration of petai ethanol extract compared to positive control (p <0.05). This study concludes that Petai ethanol extract was effective in reducing the number of atherosclerotic foam cells formed.
Expression of Plasma miRNA-133a is Significantly Lower in Acute Coronary Syndrome (ACS) than in Healthy/Non-ACS Subjects Rachmawati, Ermin; Sargowo, Djanggan; Saputra, Indra Wahyu; Riskiyah, Riskiyah; Handirosiyanto, Ikhwan; Hakim, Arief Rachman; Ismail, Mahrus; Tarsadi, Tarsadi; Maulana, Syafiq; Ahdi, Iwal Reza; Puspitasari, Alvina; Wardhani, Syanindita Puspa
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3243

Abstract

BACKGROUND: The current biomarker diagnostic modality for acute coronary syndrome (ACS), cardiac troponin, has several limitations. Emerging studies showed that micro-RNA (miR)-133a was released from infarcted heart to circulation, yet the diagnostic value of miR-133a in ACS demonstrated a conflicting result. Therefore, this study was conducted to investigate the potency of plasma miR-133a as a biomarker candidate of ACS.METHODS: This was a case-controlled study, involving ACS and control subjects. The sociodemographic and clinical characteristics were assessed through medical records. A final of 39 ACS and 31 control subjects (consist of healthy and non-ACS subjects) passed the selection procedure by demonstrating a high purity of RNA. miR-133a from ACS and control subjects were detected by quantitative polymerase chain reaction (qPCR). Expression of miR-133a was evaluated for sensitivity and specificity as an ACS biomarker diagnostic using the receiver operating characteristic (ROC) curve.RESULTS: Plasma miR-133a expression was stably found in ACS subjects. The plasma miR-133a level was lower in ACS than in control subjects. miR-133a effectively distinguished ACS subjects from healthy subjects (AUC=0.911) and exhibited high diagnostic performance, with a sensitivity of 87.1% and specificity of 100% at a cut-off value of 44.035. In an extended model including both control subjects (healthy and non-ACS with comorbid conditions), miR-133a maintained diagnostic significance (AUC=0.874), showing sensitivity of 76.9% and specificity of 100% at a cut-off value of 11.69.CONCLUSION: Plasma miR-133a is significantly lower and effectively distinguishes ACS patients from both healthy individuals and non-ACS individuals with comorbid, with a cut-off value of 11.69. Therefore, plasma miR-133a is suggested to be a good candidate for diagnostic biomarkers of ACS.KEYWORDS: circulating miRNA, miRNA-133a, acute coronary syndrome, diagnostic biomarker
Co-Authors Ahdi, Iwal Reza Arief Rachman Hakim Dian Yuliartha Lestari Djanggan Sargowo Ermin Rachmawati Indra Setiawan Ismail, Mahrus Maulana, Syafiq Nisa, Seftiana Khaerun Puspitasari, Alvina riskiyah, Riskiyah - Saputra, Indra Wahyu Tarsadi, Tarsadi Wardhani, Syanindita Puspa